Nuclear magnetic resonance (NMR) studies of complex cellular responses: isotopomer sub-spaces, 'lost' ATP and 'tunable' anisotropy

复杂细胞反应的核磁共振 (NMR) 研究：同位素子空间、“丢失”ATP 和“可调”各向异性

• 批准号: DP0877789
• 负责人: Em/Prof Philip Kuchel
• 金额: $ 34.73万
• 依托单位: The University of Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2008
• 资助国家: 澳大利亚
• 起止时间: 2008-04-01 至 2011-04-01
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0877789
• 关键词: Nuclear; magnetic; resonance; NMR; studies

Red blood cells (RBCs) transport oxygen around the body but they have other roles that are mediated by complex interconnecting metabolic pathways that generate myriad metabolites including ATP. A longstanding conundrum is the inability to account for ~60% of ATP turnover in human RBCs. Processes that may consume this 'lost' ATP, include autonomous motion of the cell membrane called 'flickering', and maintenance of the biconcave-disc shape. NMR spectroscopy of quadrupolar nuclei in chiral aligned media, and isotopomer analysis will be used to define the kinetics of metabolism and membrane processes and thus help define the molecular basis of major blood disorders.

红细胞（rbc）在全身运输氧气，但它们还有其他作用，这些作用由复杂的相互连接的代谢途径介导，产生包括ATP在内的无数代谢物。一个长期存在的难题是无法解释人类红细胞中约60%的ATP周转量。可能消耗这些“丢失的”ATP的过程包括被称为“闪烁”的细胞膜的自主运动，以及双凹盘形状的维持。手性排列介质中四极核的核磁共振波谱和同位素分析将用于确定代谢和膜过程的动力学，从而有助于确定主要血液疾病的分子基础。