Classification of Phosphate Removal Bacteria and Improvement of Phosphate Removal in the Anaerobic-Aerobic Activated Sludge Process

厌氧-好氧活性污泥法除磷菌的分类及除磷效果的改进

• 批准号: 01550423
• 负责人: MATSUO Tomonori
• 金额: $ 1.34万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01550423/
• 关键词: Biological phosphate removal; Anaerobic; aerobic activated sludge; Phosphate accumulating bacteria; Acetate metabolism; Propionate metabolism; PHB; PHV; Acinetobactor; リン蓄積菌の検策; グルタミン酸代謝; API20Eシステム

The enhanced biological phosphate removal in the anaerobic-aerobic activated sludge process is a very resources and energy saving method to remove phsphate from wastewater like sewage. From the previous researches, the anaerobic uptake of the organic substances like acetic acid, propionic acid, and other organics is thought to be one of the key mechanisms of the phosphate accumulating bacteria. In the present research, experimental investigations are carried out to classify the dominant bacterial flora in the anaerobic-aerobic activated sludge, and to identify the end products of the anaerobic uptake mechanisms of the organic substances. The important results are summarized as follows:(1)In the enhanced phosphate removal process, 18 to 50 per cent of total isolates were identified as Acinetobacter calcoaceticus var.lwoffi by the API 20E system.(2)In the anaerobic acetate uptake, the acetate was converted into the PHB stored in the biomass in accordance with the degradation of carbohydrate in the biomass.(3)In case of the propionic acid uptake, both PHB and PHV were formed to be accumulated in the biomass.(4)A conceptual metabolic pathway of the propionic acid conversion into PHV was postulated well quantitatively.(5)PHB and PHV are biodegradable plastics.

厌氧-好氧活性污泥法强化生物除磷是一种非常节能的去除污水等废水中磷的方法。以往的研究认为，对乙酸、丙酸等有机物的厌氧吸收是聚磷菌的关键机制之一。本研究对厌氧-好氧活性污泥中的优势菌群进行了分类，并对厌氧吸收有机物的最终产物进行了鉴定。主要研究结果如下：(1)在强化除磷过程中，API20E系统鉴定出18%~50%的菌株为醋酸钙不动杆菌lwoffi。(2)在厌氧醋酸盐吸收过程中，醋酸盐根据生物体中碳水化合物的降解转化为储存在生物体中的PHB。(3)在丙酸吸收过程中，PHB和PHV都形成了积累在生物体中的PHB。(4)从定量上推测了丙酸转化为PHV的概念性代谢途径。(5)PHB和PHV是可生物降解的塑料。

项目成果

Tsuzuki, Y., Mino, T. and Matsuo, T.: "Phosphorus Removal and Bacterial Flora of Anaerobic-Aerobic Activated Sludge Process" Proc. of Environ. & Sani. Eng. Research. Vol. 24. 197-208 (1989)

Tsuzuki, Y.、Mino, T. 和 Matsuo, T.：“厌氧-好氧活性污泥工艺的除磷和细菌菌群”Proc。

佐藤 弘泰,味埜 俊,松尾 友矩: "Biochemical Mechanism of Anearobic Glutamate Uptake in the Enhanced Biological Phosphrus Remoral Process" 第15回国際水質汚濁研究会議プレ・プリント. 531-534 (1990)

Hiroyasu Sato、Shun Mino、Tominori Matsuo：“增强生物磷去除过程中厌氧谷氨酸吸收的生化机制”第 15 届国际水污染研究会议预印本 531-534。

Sato, H., Mino, T. and Matsuo, T: "Biochemical Mechanism of Anaerobic Glutamate Uptake in the Enhanced Biological Phosphorus Removal Process" Poster Paper Pre-print, 16th IAWPRC Kyoto Conference, July 29-August 3, 1990, Kyoto, Japan. 531-534 (1990)

Sato, H.、Mino, T. 和 Matsuo, T：“增强型生物除磷过程中厌氧谷氨酸摄取的生化机制”海报论文预印本，第 16 届 IAWPRC 京都会议，1990 年 7 月 29 日至 8 月 3 日，京都​​，

佐藤弘泰,味埜俊,松尾友矩: "嫌気好気汚泥のグルタミン酸代謝に関する研究" 土木学会第44回年次学術講演会(平成元年10月). II. 1000-1001 (1989)

Hiroyasu Sato、Shun Mino、Tominori Matsuo：“厌氧和好氧污泥中谷氨酸代谢的研究”第 44 届日本土木工程师学会学术会议（1989 年 10 月 II）。

都筑 良明,味埜 俊,松尾 友矩: "嫌気好気式活性汚泥法のリン除去と活性汚泥細菌相" 土木学会 衛生工学研究論文集. 25. 197-208 (1989)

Yoshiaki Tsuzuki、Shun Mino、Tominori Matsuo：“厌氧和好氧活性污泥法除磷和活性污泥细菌菌群”日本土木工程学会卫生工程研究杂志 25. 197-208 (1989)。