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The Influence of Cell-Proliferating Stimuli on Carcinogen-Induced Chromosome Aberrations and Sister chromatid Exchanges.

细胞增殖刺激对致癌物诱导的染色体畸变和姐妹染色单体交换的影响。

基本信息

批准号：
01570196
负责人：
UEDA Norifumi
金额：
$ 1.02万
依托单位：
Ehime University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

Mitogenic stimulation by cell growth factor can increase the susceptibility to chemical carcinogenesis as seen in various organs and tissues. Therefore, we examined the influence of cell-proliferating stimuli on the incidence of carcinogen-induced CA and SCE. DMBA and NMU were given to L-E and S-D male rats at a dose of 50mg/kg body weight into a caudal vein. As hemopoietic stimuli, anemia and polycythemia were induced by removal of whole blood by cardiac puncture and transfusion with washed red blood cells. Chromosome specimens prepared from the femur bone marrow by standard method. BrdU was implanted subcutaneously in the abdomen 24 hours before the rats were killed. The control levels of CA and SCE were regarded as 0.09<plus-minus>0.03% and 5.80<plus-minus>s.37 per cell. In normal, anemic, polycythemic (PC) and PC+erythropoietin (EP 6 unit) rats at 6 hours, the incidence of CA induced by DMBA was 27.6<plus-minus>3.7, 45.3<plus-minus>5.7, 17.3<plus-minus>3.5 per cell respectively; of SCE, 9.11<plus-minus>2.47, 11.24<plus-minus>2.79, 6.87<plus-minus>2.47 and 12.03<plus-minus>3.70. NMU-induced CA incidence was 32.8<plus-minus>3.5, 55.6<plus-minus>4.3, 24.4<plus-minus>5.0 and 46.7<plus-minus>2.8; of SCE, 8.90<plus-minus>1.81, 18.63<plus-minus>3.12, 7.59<plus-minus>1.70 and 18.10<plus-minus>3.27. The intrachromosomal distribution of CA and SCE in various hemopoietic conditions were similar, accumulating along the 40% region of chromosome 1 and the 30% and 50% regions of chromosome 2. However, the peaks of CA and SCE were enhanced in anemia and suppressed in polycythemia. Thus DMBA-and NMU-induced CA and SCE depend on certain cell-proliferating stimuli and seem to be related phenomena. Liver cel

细胞生长因子对有丝分裂的刺激可以增加化学致癌的敏感性，在不同的器官和组织中都可以看到。因此，我们研究了细胞增殖刺激对致癌物诱导的CA和姐妹染色单体交换发生率的影响。L-E和S-D雄性大鼠按50 mg/kg体重经尾静脉注射二甲基丁酸和N-甲基-D-天冬氨酸。作为造血刺激因素的贫血和红细胞增多症是通过心脏穿刺术去除全血并用洗涤过的红细胞输注而引起的。采用标准方法从股骨骨髓制备染色体标本。大鼠处死前24小时于腹部皮下埋植BrdU。CA和SCE的控制水平分别为每细胞0.09+-0.03%和5.80+-37%。在正常、贫血、红细胞增多症(PC)和PC+EP6单位(EP6单位)大鼠6小时内，DMBA诱发CA的发生率分别为27.6、3.7、45.3、5.7、17.3、3.5个/细胞，SCE、9.11、11.24、2.47、11.24、2.79、6.87、6.87个/细胞；2.47和12.03；正负3.70。NMU诱发的CA发病率分别为32.8%、8.90%、18.63%、1.81%、18.63%、3.12%、7.59%和18.10%；正负；3.27。在不同的造血状态下，CA和姐妹染色单体在染色体内的分布相似，主要集中在1号染色体的40%区域和2号染色体的30%和50%区域，但在贫血时CA和姐妹染色单体的峰值被增强，而在红细胞增多症中被抑制。因此，DMBA和NMU诱导的CA和SCE依赖于某些细胞增殖刺激，似乎是相关的现象。肝细胞