Effects of Opioid Peptides on the Regulation of Vasopressin Secretion.

阿片肽对加压素分泌调节的影响。

• 批准号: 01570633
• 负责人: OISO Yutaka
• 金额: $ 0.9万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570633/
• 关键词: Vasopressin; Opioid peptides; Gamma-aminobutyric acid; パゾプレシン; 分泌制御; 容量受容体; 延髓孤束核; オピオイド受容体サブクラス

1. The role of opioid kappa-, mu- and delta-receptor in the secretion of arginine vasopressin (AVP) was first studied. Opioid kappa- and mu- receptor agonists significantly suppressed plasma AVP increased by hyperosmolar or hypovolemic stimulation. Opioid delta-receptor agonist did not suppressed plasma AVP induced by hypovolemia. These results suggest that suppressive activity of opioid receptor agonist for AVP secretion depends on its biological subclass.2. Secondly, we examined the effect of gamma-aminobutyric acid (GABA) and naloxone, a potent opioid antagonist, on AVP secretion in conscious rats in order to study the relationship of GABA and endogenous opioid peptides in the regulation of AVP secretion. Intracerebroventricular administration of GABA caused a time and dose dependent decrease in the plasma concentration of AVP that was elevated by hypertonic saline injection, whereas it did not affect the basal AVP. Pretreatment with naloxone significantly attenuated the inhibitory effect of GABA on AVP release. These results suggest that GABA produces an inhibition of AVP release stimulated by hypertonic saline, and that this inhibitory effect may be mediated at least in part by the endogenous opioid systems.

1.首次研究了阿片κ、μ和δ受体在精氨酸加压素（AVP）分泌中的作用。阿片类κ和μ受体激动剂显著抑制高渗或低血容量刺激引起的血浆AVP升高。阿片δ受体激动剂不能抑制低血容量引起的血浆AVP。这些结果提示阿片受体激动剂对AVP分泌的抑制作用依赖于其生物学亚型.其次，我们观察了γ-氨基丁酸（GABA）和阿片拮抗剂纳洛酮对清醒大鼠AVP分泌的影响，以探讨GABA和内源性阿片肽在AVP分泌调节中的关系。脑室注射GABA引起AVP血浆浓度呈时间和剂量依赖性降低，而高渗盐水注射升高AVP血浆浓度，但不影响基础AVP。纳洛酮预处理可明显减弱GABA对AVP释放的抑制作用。这些结果表明，GABA产生抑制AVP释放刺激高渗盐水，这种抑制作用可能至少部分介导的内源性阿片系统。

项目成果

Kazuo Otake, Kunikazu Kondo and Yutaka Oiso.: "Possible involvement of endogenous opioid peptides in the inhibition of arginine vasopressin release by gamma-aminobutyric acid in conscious rats." Neuroendocrinology.

Kazuo Otake、Kunikazu Kondo 和 Yutaka Oiso.：“内源性阿片肽可能参与清醒大鼠中γ-氨基丁酸对精氨酸加压素释放的抑制。”

Yutaka Oiso: "Comparison of suppressive effects of opioid kappaー,muー and deltaーreceptor agonists on arginine vasopressin secretion." Neuroendocrinology Letters. 12. 443-448 (1990)

Yutaka Oiso：“阿片类药物 kappa、mu 和 delta 受体激动剂对精氨酸加压素分泌的抑制作用的比较。神经内分泌学快报。”12. 443-448 (1990)

Kazuo Otake: "Possible involvement of endogenous opioid peptides in the inhibition of arginine vasopressin release by γーaminobutyric acid in conscious rats." Neuroendocrinology. (1991)

Kazuo Otake：“内源性阿片肽可能参与γ-氨基丁酸在清醒大鼠中抑制精氨酸加压素的释放（1991）。”

Yutaka Oiso, Kunikazu kondo, Masahumi Ito, Yasumasa Iwasaki and Kazuo Otake.: "Comparison of suppressive effects of opioid kappa-, mu- and delta-receptor agonists on arginine vasopressin secretion." Neuroendocrinology Letters. 12. 443-448 (1990)

Yutaka Oiso、Kunikazu kondo、Masahumi Ito、Yasumasa Iwasaki 和 Kazuo Otake.：“阿片类 kappa、mu 和 delta 受体激动剂对精氨酸加压素分泌的抑制作用比较。”