Physiological characterization of the human vasopressin transgenic rats.

为了研究慢性高血管加压素血症遗传模型的生理后果，将小鼠异位硫蛋白-人精氨酸加压素(AVP)基因导入大鼠生殖系，获得转基因大鼠。对一个稳定的转基因家系进行了多代遗传分析。Southern blotting证实了人AVP基因的表达，并导致大脑中AVP的数量增加。与正常大鼠相比，中枢神经系统分泌的AYP导致血浆中激素浓度的增加和尿液中AYP排泄量的增加，增加了五到十倍。在肾脏中发现异位激素的产生。转基因大鼠在基础状态下血浆钠水平无明显下降，但24小时尿量减少。这些数据可能表明转基因大鼠肾脏的抗利尿活性增加。

To study the physiologic consequences of a genetic model of chronic hypervasopressinemia, transgenic rat was produced by induction of a mouse nietallothionein-human arginine vasopressin (AVP) gene into the germ line of rat. One stable transgenic pedigree was analyzed through several generations. Expression of the human AVP gene was demonstrated by Southern blotting and resulted in increased amounts of AVP in brains. Secretion of AYP from the central nervous system results in an increased concentration of the hormone in the plasma and in an increased excretion in the urine in amounts five to ten times compared to those of normal rats. Ectopic hormone production was found in the kidney. Transgenicrat showed no significant decrease in plasma Na level but decreased 24-hour urine volume under basal conditions. These data might suggest increased antidiuretic activity on kidney in transgenic rat.