Physiological characterization of the human vasopressin transgenic rats.

人加压素转基因大鼠的生理特征。

• 批准号: 08457263
• 负责人: OISO Yutaka
• 金额: $ 4.8万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457263/
• 关键词: arginine vasopressin; transgenic rat; hypervasopressinemia; antidiuretic activity; ectopic hormone production; 遺伝子発現; 疾患モデル; 下垂体後葉ホルモン

To study the physiologic consequences of a genetic model of chronic hypervasopressinemia, transgenic rat was produced by induction of a mouse nietallothionein-human arginine vasopressin (AVP) gene into the germ line of rat. One stable transgenic pedigree was analyzed through several generations. Expression of the human AVP gene was demonstrated by Southern blotting and resulted in increased amounts of AVP in brains. Secretion of AYP from the central nervous system results in an increased concentration of the hormone in the plasma and in an increased excretion in the urine in amounts five to ten times compared to those of normal rats. Ectopic hormone production was found in the kidney. Transgenicrat showed no significant decrease in plasma Na level but decreased 24-hour urine volume under basal conditions. These data might suggest increased antidiuretic activity on kidney in transgenic rat.

为了研究慢性高加压素血症遗传模型的生理学后果，将小鼠镍硫蛋白-人精氨酸加压素（AVP）基因导入大鼠生殖细胞系，制备了转基因大鼠。通过几代分析一个稳定的转基因家系。通过Southern印迹证明了人AVP基因的表达，并导致脑中AVP的量增加。从中枢神经系统分泌AYP导致血浆中激素浓度增加，并且与正常大鼠相比，尿中排泄量增加5至10倍。在肾脏中发现异位激素产生。转基因大鼠在基础条件下血浆钠水平没有显著降低，但24小时尿量减少。这些数据可能表明转基因大鼠肾脏的抗利尿活性增加。

项目成果

Hisashi Yokoi et al.: "Antiserum against neuropeptide FF augments vasopressin release in conscious rats" Peptides. 19. 393-395 (1998)

Hisashi Yokoi 等人：“针对神经肽 FF 的抗血清增强了清醒大鼠中加压素的释放”肽。

Hiroshi Arima et al.: "Regulation of vasopressin synthesis and release by area postrema in rats" Endocrinology. (in press).

Hiroshi Arima 等人：“大鼠后区加压素合成和释放的调节”内分泌学。

大磯ユタカ: "水代謝異常とバゾプレシン" 日本内科学会雑誌. 86. 1645-1649 (1997)

Yutaka Oiso：“水代谢异常和加压素”日本内科学会杂志 86. 1645-1649 (1997)。

Yasumasa Iwasaki et al.: "Positive and nagative regulation of the vasopressin gene promoter" Endocrinology. 138. 5266-5274 (1997)

Yasumasa Iwasaki 等人：“加压素基因启动子的正向和负向调节”内分泌学。

Y.Oiso and Y.Iwasaki: "Vasopressin and related disorders.(in Japanese)" J.Jap.Soc.Intern.Med.86. 1645-1649 (1997)

Y.Oiso 和 Y.Iwasaki：“加压素和相关疾病。（日语）”J.Jap.Soc.Intern.Med.86。