The regenerative therapy for central diabetes insipidus

中枢性尿崩症的再生疗法

• 批准号: 22390188
• 负责人: OISO Yutaka
• 金额: $ 8.99万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390188/
• 关键词: 再生医学; 中枢性尿崩症; 生理活性; バゾプレシン; 再生医療; 細胞移植; マウスES細胞; 無血清浮遊培養法; 視床下部前駆細胞

It is known mouse embryonic stem cell can be developed into hypothalamic lineage (ES-hypo) invitro. In this study, we have improved the method to have vasopressin neurons more effectively. Central diabetes insipidus manifests polyuria and polydipsia which results from the deficit in the hypothalamic area that produce vasopressin. We have developed a regenerativetherapy using ES-hypo in the model animal, Brattleboro rat. The heterograft of ES-hypo in the brain survived more than six weeks, and recipients reduced urine volume by 40% compared to the negative control group.

已知小鼠胚胎干细胞在体外可分化为下丘脑系（ES-hypo）。在这项研究中，我们改进了方法，使加压素神经元更有效。中枢性尿崩症表现为多尿和烦渴，这是由于产生加压素的下丘脑区域的缺陷造成的。我们已经开发了一种使用ES-hypo的再生治疗模型动物，Brattleboro大鼠。在脑中的ES-次氯酸钠的异种移植物存活超过六周，与阴性对照组相比，受体的尿量减少了40%。

项目成果

Posttranscriptional regulation of vasopressin mRNA in a mouse model for familial neurohypophysial diabetes insipidus.

家族性神经垂体尿崩症小鼠模型中加压素 mRNA 的转录后调节。

• 发表时间: 2010
• 作者: 大磯ユタカ;他
• 通讯作者: 他

BiP mRNA expression is upregulated by dehydration in vasopressin neurons in the hypothalamus in mice

• DOI: 10.1016/j.peptides.2011.12.011
• 发表时间: 2012-02-01
• 期刊: PEPTIDES
• 影响因子: 3
• 作者: Hagiwara, Daisuke;Arima, Hiroshi;Oiso, Yutaka
• 通讯作者: Oiso, Yutaka

Protective Effect of Hedgehog Signaling on Cytokine-Induced Cytotoxicity in Pancreatic Beta-Cells.

Hedgehog 信号传导对胰腺 β 细胞细胞因子诱导的细胞毒性的保护作用。

• 发表时间: 2010
• 期刊: Experimental and Clinical Endocrinology & Diabetes
• 影响因子: 1.8
• 作者: 大磯ユタカ;他
• 通讯作者: 他

Lesion of area postrema attenuated hyperphagic responses to glucoprivation, but not transcriptional activation of the neuropeptide Y gene in rats

• DOI: 10.1097/wnr.0b013e3283556676
• 发表时间: 2012-08-01
• 期刊: NEUROREPORT
• 影响因子: 1.7
• 作者: Ozawa, Yoshiharu;Arima, Hiroshi;Oiso, Yutaka
• 通讯作者: Oiso, Yutaka

TNFα increases hypothalamic PTP1B activity via the NFκB pathway in rat hypothalamic organotypic cultures

• DOI: 10.1016/j.regpep.2011.11.010
• 发表时间: 2012-02-10
• 期刊: REGULATORY PEPTIDES
• 作者: Ito, Yoshihiro;Banno, Ryoichi;Oiso, Yutaka
• 通讯作者: Oiso, Yutaka