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Experimental study of vascularized bone grafts in rabbit

兔血管化骨移植的实验研究

基本信息

批准号：
01570845
负责人：
TAMAI Susumu
金额：
$ 1.28万
依托单位：
Nara Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

The purpose of this study was to quantify the bone blood flow in long bone transplanted to another long bone, and investigate bone dynamics under different mechanical and biological environments using rabbits. Three experimental groups were performed. Vascularized group : The right 2nd metatarsal bone was elevated with the saphenous vessels as a vascularized bone graft. The graft was transplanted to a 1.5cm defect prepared in the femoral diaphysis and fixed with an external fixator. Conventional group : nonvascularized metatarsal bone graft of which the surrounding soft tissues were stripped away was transplanted with the same manner. Non weight-bearing group : Vascularized metatarsal bone graft was transplanted into the femoral subcutaneous tissue.The radioactive microsphere method was used for the measurement of the bone blood flow. In the vascularized group the blood flow of the graft had immediately increased. At one week postoperatively, the mean blood flow was 11.8ml/min/100g of bone, at 3 weeks, 17.4. at 6 weeks, 13.3. At 9 weeks, the mean blood flow significantly decreased to 3.9 and at 12 weeks, 3.6. The diameter of the graft increased until 6 weeks, as larger as 1.6 times larger than the contralateral metatarsal bone, but subsequently no progression of the hypertrophy was noted.In the conventional group, the mean blood flow gradually increased to 6.8 ml/min/100g at nine weeks postoperatively and it was 6.4 ml/min/100g at 12 weeks.In the non weight-bearing group, the mean flow in the bone grafts immediately increased to 10.4ml/min/100g at 1 week postoperatively. It was 7.0ml/min/100g at 3 weeks and 6.5ml/min/100g at 6 weeks.Our experiments demonstrated that hypertrophy occurs in response to biomechanical and biological demands in the presence of a rich bone blood flow.

本研究的目的是定量兔长骨移植到另一长骨的骨血流量，并研究在不同的力学和生物学环境下的骨动力学。进行了三个实验组。带血管组：右侧第2跖骨带隐血管抬高作为带血管骨移植。将移植物移植到股骨干制备的1.5cm缺损处，并用外固定器固定。常规组：剥离周围软组织的非血管化跖骨移植物以相同的方式移植。非负重组：将带血管蒂的跖骨移植到股骨皮下，用放射性微球法测定骨血流量。在血管化组中，移植物的血流量立即增加。术后1周，平均血流量为11.8ml/min/100 g骨，3周为17.4。第6周的时候是13.3在第9周，平均血流量显著下降至3.9，在第12周，3.6。术后6周，移植骨直径逐渐增大，达到对侧跖骨直径的1.6倍，但随后没有观察到肥大的进展，常规组术后9周平均血流量逐渐增加至6.8ml/min/100 g，12周为6.4ml/min/100 g，非负重组术后12周平均血流量逐渐增加至6.4ml/min/100 g。术后1周移植骨内平均血流量立即增加至10.4ml/min/100 g。3周时为7.0ml/min/100 g，6周时为6.5ml/min/100 g。