DEVELOPMENT OF ARTIFICIAL TENDON AND LIGAMENT BY FUNCTIONAL DESIGN OF POLYMER SURFACE

通过聚合物表面功能设计开发人工肌腱和韧带

• 批准号: 06557085
• 负责人: TAMAI Susumu
• 金额: $ 10.37万
• 依托单位: NARA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06557085/
• 关键词: polymer; bone bonding; hydroxyapatite; 骨結合; 表面グラフト重合; 生体材料; 表面改質

・ We have found calcium phosphate was rapidly deposited in the form of hydroxyapatite (HA) crysralline thin layrs onto the polymer substrate, having phosphate graft chains from the supersaturated solution. Interfacial bonding between the deposited HA layr and the polymer substrate was achieved by the grafted chains. Solubility of the deposited HA layr in aqueous medium was reduced by the increase in the crystallinity of HA deposits.・ As an in vivo study, a phosphate-containing polymer was covalently immobilized onto a polyethylene (PE) rod and a poly (ethylene terephthalate) (PET) thread. Significant enhancement was observed for direct contact of the implant surface with a newly formed bone both for the grafted only and the further HA-deposited PE rods in comparison with the untreated PE at 4 to 6 weeks after implantation. The rats implanted with the PET thread in the femur were sacrificed 3 and 6 weeks after implantation. Statistically significant differences were observed for the unt … More reated vs. the grafted+HA-deposited at 6 weeks after implantation. These results suggest that the phosphate polymer chains grafted on the PE and PET surfaces effectively induced nucleation and growth of HA crystals.・ We have studied the bone formation of the marrow cell/polymer composites and also investigated the gene expression analysis relating the bone formation on and around the composites. Recently, we confirmed the in vitro bone formation by marrow cell culture, and the bone formation (osteoblastic activity) was comparable to that of in vivo bone studied by physiochemical, biochemical and gene expression analysis. The in vitro bone tissue can be formed on various biomaterials and interestingly, the in vitro bone has extensive bone forming capability after in vivo transplantation. As described we accomplished basic analysis of marrow cell composites.・ Concerning the clinical application of the polymer in orthopedic field, we used PLLA ligament for the cases of knee ligament injury and short term follow up results were satisfactory. Less

·我们已经发现磷酸钙以羟基磷灰石（HA）结晶薄层的形式快速沉积在聚合物基底上，具有来自过饱和溶液的磷酸盐接枝链。通过接枝链实现了沉积的HA层与聚合物基体之间的界面结合。沉积的HA层在水介质中的溶解度通过HA沉积物的结晶度的增加而降低。作为体内研究，将含磷酸盐的聚合物共价固定在聚乙烯（PE）棒和聚（对苯二甲酸乙二醇酯）（PET）线上。在植入后4 - 6周，与未处理的PE相比，对于仅移植的和进一步HA沉积的PE棒，观察到植入物表面与新形成的骨的直接接触显著增强。在股骨中植入PET线的大鼠在植入后3周和6周被处死。观察到unt的统计学显著差异 ...更多信息 植入后6周，移植组与移植+HA沉积组相比。这些结果表明，接枝到PE和PET表面的磷酸酯聚合物链有效地诱导了HA晶体的成核和生长。我们研究了骨髓细胞/聚合物复合材料的骨形成，并研究了与复合材料上和周围骨形成相关的基因表达分析。最近，我们通过骨髓细胞培养证实了体外骨形成，并且骨形成（成骨细胞活性）与通过理化、生化和基因表达分析研究的体内骨相当。体外骨组织可以在各种生物材料上形成，有趣的是，体外骨在体内移植后具有广泛的骨形成能力。如上所述，我们完成了骨髓细胞复合物的基本分析。·关于聚乳酸在骨科领域的临床应用，我们将聚乳酸韧带用于膝关节韧带损伤的病例，短期随访结果令人满意。少

项目成果

H.Ohgushi,Y.Dohi,S.Tamai and S.Tabata: "Osteogenic differentiation of marrow stromal stem cells in porous hydroxyapatite ceramics" Journal of Biomedical Materials Research. 27. 1401-1407 (1993)

H.Ohgushi、Y.Dohi、S.Tamai 和 S.Tabata：“多孔羟基磷灰石陶瓷中骨髓基质干细胞的成骨分化”生物医学材料研究杂志。

K.Kato, Y.Eika and Y.Ikada: " "Deposition of a hydroxyapatite thin layr onto a polymer surface carrying grafted phosphate polymer chains"" J.Biomed. Mat. Res. 32. 687-691 (1996)

K.Kato、Y.Eika 和 Y.Ikada：“将羟基磷灰石薄层沉积到带有接枝磷酸盐聚合物链的聚合物表面”，J.Biomed。

K. Kato, Y. Eika, Y. Ikada: "Depositior of a hydroxyapatite thin layer on to a polymer surface carrying grafted phosphate polymer chains" Journal of Biomedical Materials Research. 32・4. 687-691 (1996)

K. Kato、Y. Eika、Y. Ikada：“在携带接枝磷酸盐聚合物链的聚合物表面上沉积羟基磷灰石薄层”《生物医学材料研究杂志》32・4（1996）。

K.Kato,Y.Eika,Y.Ikada: "Deposition of a hydroxyapatite thin layer onto a polymer surface carrying grafted phosphate polmer chains" J.Biomed.Mat.Res.32. 687-691 (1996)

K.Kato，Y.Eika，Y.Ikada：“在携带接枝磷酸盐聚合物链的聚合物表面上沉积羟基磷灰石薄层”J.Biomed.Mat.Res.32。

H. Ohguchi, Y. Dohi, T. katuda, S. Tamai, S. Tabata, Y. Suwa: "Invitro bone formation by rat marrow cell culture" Journal of Biomedical Materials Research. 32・3. 333-340 (1996)

H. Ohguchi、Y. Dohi、T. katuda、S. Tamai、S. Tabata、Y. Suwa：“大鼠骨髓细胞培养的体外骨形成”《生物医学材料研究杂志》32・3（1996）。