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Experimental study of Vasculalized Nerve Allograft

同种异体血管神经移植的实验研究

基本信息

批准号：
04670903
负责人：
TAMAI Susumu
金额：
$ 1.47万
依托单位：
Grant in Aid for scientific Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

Maintenance of favorable regeneration of transplanted peripheral nerves after discontinuation of immunosuppressant therapy was examined by means of allotransplantation of the sciatic nerve in rats. Brown-Norway rats (B-N : RT-1^n) were used as donors and Fisher rats (F344 : RT-1^1) were used as recipients. These two strains were MHC incompatible each other. Vascularized nerve grafts (VNG) and free nerve grafts (FNG) were compared. For immunosuppression, 0.5mg/kg of FK506 was intramuscularly injected once daily from the day of transplantion. On the basis of treatment with FK506,0 (no treatment), 4,6,8 and 12 week treatment groups were prepared. Each group consisted of 6 planted rats, and the animals were examined in the 16th and 20 weeks. Results were evaluated by choline acetyltransferase (CAT) activity, histological examination and electromyography. Axons continued to regenerate favorably after termination of treatment with the immunosuppressant. The myelin and schwann' s cells showed temporary rejection of the graft after the end of treatment with the immunosuppressant, but this change seemed to heal later. Administration of the immunosuppressant was necessary to continue until all the regenerating axons completed expension through the nerve graft. No significant differences were observed between the VNG and FNG.However, when the period of treatment with the immunosuppressant was short. CAT activity of VNG was higher than that of FNG.Hesurement of CAT activity was shown to be a simple method for the quantitative evaluation of axonal function.

通过大鼠坐骨神经同种异体移植，研究了免疫抑制剂治疗停止后移植周围神经良好再生的维持情况。Brown-Norway大鼠（B-N：RT-1 ^n）用作供体，Fisher大鼠（F344：RT-1 ^1）用作受体。这两株病毒是MHC不相容的。对带血管神经移植物（VNG）和游离神经移植物（FNG）进行比较。FK506 0.5mg/kg，从移植当天开始肌肉注射，每日1次，用于免疫抑制。在FK506治疗的基础上，设0（未治疗）、4、6、8、12周治疗组。每组6只，分别于第16周和第20周进行检查。结果通过胆碱乙酰转移酶（CAT）活性，组织学检查和肌电图进行评估。在免疫抑制剂治疗终止后，轴突继续顺利再生。髓磷脂和雪旺氏细胞在免疫抑制剂治疗结束后表现出对移植物的暂时排斥，但这种变化似乎在后来愈合。免疫抑制剂的管理是必要的，直到所有的再生轴突通过神经移植物完成扩张。VNG与FNG之间无显著性差异，但当免疫抑制剂治疗时间较短时，VNG与FNG之间无显著性差异。VNG的CAT活性高于FNG，说明CAT活性的测定是一种简便的定量评价轴突功能的方法。