Experimental Study on End-to-Side Neurorrhaphy

端侧神经缝合术的实验研究

• 批准号: 10671376
• 负责人: TAMAI Susumu
• 金额: $ 1.28万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671376/
• 关键词: REGENERATION; NERVE; END-TO-SIDE NEURORRHAPHY; CHOLINE ACETYLTRANSFERASE ACTIVITY; 神経再生; 神経栄養因子

Object : The mechanism of collateral spouting axons from an uninjured donor nerve after end-to-side nerve repair was investigated in motor nerves of rats, with special reference to neurotrophins related to nerve regeneration. In addition, the growth cone formation which is formed at the tip of the regenerating nerve was examined.Methods : The transected medial gastrocnemius nerve (MGN) was transferred to the side of intact lateral gastrocnemius nerve (LGN) using a Y-shaped silicone tube. No epineurial window, suture damage or perineurial slit was made in the LGN at the site of coaptation. Other nerves in the leg were transected and ligated. At 3, 7 or 14 days later, the MGN with LGN was transected around the coaptation site, and was stained immunohistologically. In the results, the NT-3 and TrK C (receptor of NT-3) expressions were most significantly observed at 3 days postoperatively around the site of coaptation. BDNF was weakly detected at the coaptation site 3 days post-operatively. TrK B (receptor of BDNF) was not significantly detected in the specimens. Growth-associated protein 43 (GAP-43) which is a marker of growth cone formation, was observed at the site of coaptation in the LGN at 7 days postoperatively, and also in the MGN at 14 days at the site of coaptation.Conclusions : We concluded that motor nerve regeneration due to collateral spouting of axons after end-to-side nerve repair is possible. In this situation, we showed the involvement of at least one neurotrophin, NT-3, in the process of collateral sprouting of motor nerves.

目的：研究大鼠运动神经端侧修复后未损伤供体神经侧支喷射轴突的机制，特别是神经营养因子与神经再生的关系。此外，还检查了再生神经顶端形成的生长锥形成。方法：采用y型硅胶管将横断的腓肠肌内侧神经（MGN）移植到完整的腓肠肌外侧神经（LGN）一侧。在闭合部位，LGN未见神经外窗、缝合线损伤或神经外缝。将腿部的其他神经横切并结扎。3、7、14天后，在附着部位周围横切带有LGN的MGN，并进行免疫组织学染色。结果显示，NT-3和TrK C （NT-3的受体）的表达在术后3天最显著。术后3天在包合部位检测到较弱的BDNF。BDNF受体TrK B在标本中未检测到。生长相关蛋白43 （growth -associated protein 43, GAP-43）是生长锥形成的标志，在术后7天在LGN的适应部位被观察到，在术后14天在MGN的适应部位也被观察到。结论：端侧神经修复后，通过轴突侧支喷涌实现运动神经再生是可行的。在这种情况下，我们发现至少有一种神经营养因子NT-3参与了运动神经侧支发芽的过程。