A Study on the Factors Controlling the Functions of Ovarian and Endometrial Cells

卵巢和子宫内膜细胞功能控制因素的研究

基本信息

批准号：
01570921
负责人：
ASO Takeshi
金额：
$ 1.47万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

It is well recognized that the functions in a system of organs are controlled by feedback mechanisms in order to maintain the homeostasis and exhibit physiological effects. In addition to these general control, the importance of the local mutual communication between organ to organ, as well as cell to cell(paracrine/autocrine system)should be emphasized. In the present study, the mechanism of action of various factors which are supposed to play stimulatory or inhibitory roles on reproductive organs including pituitary, ovary and endometrium, are investigated.As the results of the study on local factors in pituitary gland, the paracrine role of angiotensin in GN-RH induced prolactin release from anterior cells was indicated.The direct effects of bromocriptine, insulin-like growth factors(IGFS)and endothelin(ET)-1 were observed in cultured porcine granule-sa cells. Bromocriptine and IGF-II significantly activated proliferation, differentiation and steroidogenic capability of the cells, through the extracellular CAMP accumulation. The presence of non-selective ET receptor was identified in porcine granulosa cells coupled with phosphoinositide hydrolysis and Ca metabolism. ET-1 was suggested to have some role on progesterone production in the cell.The physiological dose of danazol, which has been clinically used for the treatment of endometriosis, increased the number of lysosomes of the cytoplasm in the cultured human endometrial stromal cells, and destroyed cell organelles and cytoskeleton were finally induced.The possible action and mechanism of IGFs and endothelin on the cultured human decidual cell function to produce prolactin, and ultimate intrauterine fetal growth were investigated in vivo and in vitro. The prolactin release from cultured human decidual cells was enhanced by IGF-I, and significantly elevated maternal ET-1 levels were observed in the last trimester pregnancy.

众所周知，器官系统中的功能由反馈机制控制，以维持体内平衡并表现出生理效应。除这些一般控制外，还应强调器官之间的局部相互通信以及细胞与细胞之间的局部相互通信的重要性（旁分泌/自分泌系统）。在本研究中，研究了各种因素的作用机制，这些因素应在包括垂体，卵巢和子宫内膜在内的生殖器官中起刺激性或抑制性作用。在培养的猪颗粒-SA细胞中观察到因子（IGF）和内皮素（ET）-1。溴o不般的IGF-II通过细胞外cAMP积累显着激活了细胞的增殖，分化和类固醇生成能力。在猪颗粒细胞中鉴定出非选择性ET受体的存在与磷酸肌醇水解和Ca代谢。 ET-1被认为在细胞中的孕激素产生中起作用。那唑醇的生理剂量已用于治疗子宫内膜异位症，增加了培养的人子宫内膜间质细胞中细胞质的溶酶体数量，并破坏了细胞细胞器和人类的作用。在体内和体外研究了产生催乳素和最终子宫内胎儿生长的细胞功能。通过IGF-I增强了从培养的人codiDual细胞中的催乳素释放，在上个三个月妊娠中观察到了母体ET-1水平的显着升高。