A study on the factors controlling bone metabolism

骨代谢控制因素的研究

基本信息

批准号：
04670992
负责人：
ASO Takeshi
金额：
$ 1.34万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670992/
关键词：
bone metabolism estrogen vitamin D3 Kampo medicine osteoblast osteoclast GnRH agonist anti-cancer agent

项目摘要

The bone metabolism is cotrolled by various factors. In this study, three experiments were conducted to add new informations concerning the influences of hyposestrogenism, anti-cancer chemotherapy and progesterone on the metabolism.In the first experiment, estrogen production of the ovary was suppressed in female mature rats by gonadotropin releasing hormone agonist(GnRH-A) for 10 weeks. The mineral bone density, the findings of contact microradiogram, and osteogenic parameters were analyzed at the end of treatment period. A group of rats was administered HACHIMI-JIO-GAN (HJG), a Kampo medicine simultaneously. The increased bone absorption due to the decreased of the width of trabecular was detected in the GnRH-A treated rats. Such changes were reversed by the HJG administration by which the cell number and cell surface of osteoclast were reduced.The second experiment concerned the negative effects of cisplatin, an anti-cancer agent for gynecological malignacies on vitamin D3 mtabolism. In the patients who were given this agent exhibited marked decrease of 1,25-dihydrovitamin D3. It was indicative that the toxic action of cisplatin on the distal tubules of kidney induced the dysfuncion of vitamin D3 metabolism, and the bone loss of such patients was accelerated.The influence of progesterone on the osteoblast function was investigated in an in vitro experiment. The production of TGF-beta from the cultured human osteosarcoma cells was enhanced by progesterone. The increased mRNA expression of TGF-beta in human osteoblast was detected by northern bolt analysis in the conditions stimulated by progesterone. It is suggested that progesterone plays a role in the coupling of osteoblast and osteoclast cell functions.

骨代谢是由各种因素共同卷。在这项研究中，进行了三项实验，以增加有关降虫质主义，抗癌化学疗法和孕酮对代谢的影响的新信息。在第一个实验中，雌性成熟的大鼠在雌性成熟的大鼠中抑制了卵巢的雌激素产生的雌激素释放性激素释放激素激素（Gnrh-a）10周。在治疗期结束时，分析了矿物质骨密度，接触微放射术的发现和成骨参数。一组大鼠同时服用了坎波药Hachimi-i-io-gan（HJG）。在GNRH-A治疗的大鼠中检测到小梁宽度降低引起的骨吸收增加。 HJG给药逆转了这种变化，通过该变化减少了破骨细胞的细胞数和细胞表面。第二实验涉及顺铂的负面影响，Cisplatin是妇科恶性肿瘤对维生素D3 Mtabolismism的抗癌剂的负面影响。在给予该药物的患者中，该药物明显降低了1,25-二氢胺D3。这表明顺铂对肾脏远端小管的毒性作用诱导了维生素D3代谢的功能障碍，并加速了这种患者的骨损失。孕酮对成骨细胞功能的影响在体外实验中进行了研究。孕酮从培养的人骨肉瘤细胞中产生TGF-β细胞。在孕酮刺激的条件下，通过北螺栓分析检测到人类成骨细胞中TGF-β的mRNA表达增加。建议孕酮在成骨细胞和破骨细胞功能的偶联中起作用。