Analysis of Immunodominant Antigens of Periodontopathic Bacteria

牙周病细菌的免疫显性抗原分析

• 批准号: 01571040
• 负责人: WATANABE Hisashi
• 金额: $ 1.41万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01571040/
• 关键词: Periodontopathic Bcteria; Immunodominant Antigen; Immunoblotting; Bacteroides gingivalis; Veillonella parvula; Actinobacillus actinomycetemcomitans; A・actinomyctemcomitans; 若年性歯周炎; SDSーPAGE; イムノブロット; 歯周病原菌; Bacterordes gingivalis; ヒト歯周炎; 外膜蛋白

We have obtained the following results through this research.1. Sonicated whole cell extracts and Outer Membrane Proteins (OMP) from Bacteroides gingivalis and Veillonella parvula were analysed by the immunoblot technique using sera from 103 patients with various forms of periodontal disease and from 31 control subjects. B.gingivalis sonicatecontained 12 major hands (75-14kDa) of which the 46, 27 and 14 kDa antigens reacted more frequently with sera from adult and young adult patients with severe periodontitis compared with sera from controls and mild periodontitis patients. The OMP of B.gingivalis contained 6 main antigens of 75, 57, 51, 46, 35 and 19 kDa m. w.. The 46 kDa antigen reacted predominantly with sera from both groups of patients with severe periodontitis. V.parvula sonicate contained 11 antigens (76-13 kDa) of which the 76 kDa antigen reacted more frequently with sera from controls and patients with mild periodontitis than with sera from patients with severe periodontitis. … More Conversely, antibodies to the 39 kDa antigen (absent from OMP) were specifically associated with severe periodontitis.2. Actinobacillus actinomycetemcomitans was analysed. Sonicated whole cell extracts and outer membrane proteins from this bacterium were examined using sera from 31 young patients with localized juvenile periodontitis, 55 young adults with generalized severe periodontitis and from 31 healthy control subjects. THe sonicate contained 13 major hands (14-78 kDa) ; a greater proportion of sera from patients with generalized periodontitis reacted with 40 and 70 kDa antigens when compared with sera from localized juvenile periodontitis and controls. In contrast, a lower proportion of sera from localized juvenile periodontitis reacted with the 29 kDa antigen when compared with severe periodontitis and controls. The outer membrane proteins contained four main antigens of 19, 24, 35 and 67 kDa, which sera from all three groups. Although, so far, the finding s do not allow discrimination between the two diseases, antibody response to the 29, 40 and 70 kDa antigens of A. actinomycetemcomitans may help in the assessment of severity of the disease in patients with periodonitis. Less

通过本研究，我们取得了以下成果：1.超声处理的全细胞提取物和外膜蛋白（OMP）从牙龈拟杆菌和小韦荣氏球菌进行了分析，免疫印迹技术使用的血清从103例各种形式的牙周病和31个对照组。牙龈B菌声波培养物含有12个主要抗原带（75- 14 kDa），其中46、27和14 kDa抗原与成人和青年重度牙周炎患者血清的反应频率高于对照组和轻度牙周炎患者血清。牙龈B.gingivalis的外膜蛋白含有6种主要抗原，分子量分别为75、57、51、46、35和19 kDa。我... 46 kDa抗原主要与两组严重牙周炎患者的血清反应。小弧菌超声处理物含有11种抗原（76-13 kDa），其中76 kDa抗原与来自对照和轻度牙周炎患者的血清的反应比与来自重度牙周炎患者的血清的反应更频繁。 ...更多信息 相反，39 kDa抗原抗体（OMP中不存在）与严重牙周炎特异性相关。对伴放线放线杆菌进行了分析。超声处理的全细胞提取物和外膜蛋白从这种细菌进行了检查，使用血清从31名年轻患者局部青少年牙周炎，55名年轻人与全身性严重牙周炎和31名健康对照组。超声波处理物含有13个主要的手（14-78 kDa）;与局部青少年牙周炎和对照组的血清相比，全身性牙周炎患者的血清与40和70 kDa抗原反应的比例更大。与此相反，与严重牙周炎和对照组相比，局部青少年牙周炎的血清与29 kDa抗原反应的比例较低。外膜蛋白含有19、24、35和67 kDa的四种主要抗原，它们来自所有三个组的血清。尽管到目前为止，这一发现还不能区分这两种疾病，但对A.伴放线菌素可帮助评估牙周炎患者疾病的严重程度。少

项目成果

新田 浩: "歯周疾患関連細菌に対する血清抗体価と加齢について" 日本歯周病学会会誌. 32. 667-672 (1990)

Hiroshi Nitta：“关于针对牙周病相关细菌和衰老的血清抗体滴度”日本牙周病学会杂志 32. 667-672 (1990)。

Watanabe, H., Marsh, P. D. and lvanyi, L.: "Antigens of Actinobacillus actinomycetemcomitans identified by immunoblotting with sera from patients with localized human juvenile periodontitis and generalized sera periodontitis." Archs oral Biol. 34. 649-656

Watanabe, H.、Marsh, P. D. 和 lvanyi, L.：“通过用患有局限性人类幼年牙周炎和全身性血清牙周炎患者的血清进行免疫印迹来鉴定伴放线放线杆菌的抗原。”

H.WATANABE: "Antigens of Actinobacillus actinomyce temconitans Identified by Immanoblotting with Sera from Pafients with Locallzed Human Juvenile Poriodontitis and Generalizul Sivere Periodontitis." Archives of oral Biology. 34. 649-656 (1989)

H.WATANABE：“通过免疫印迹法从患有局限性人类青少年脊椎牙周炎和全身性严重牙周炎的患者血清中鉴定出放线菌放线菌抗原。”

I.ISHIKAWA: "Diversidad en la Respuesta del Huasped en Pacientes con Periodontitis Avanzada"

I.ISHIKAWA：“Diversidad en la Respuesta del Huasped en Pacientes con periodontitis Avanzada”

ISHIKAWA,I: "Diversidad en la nepueuta del hmeopeden pacicnts con periodontitis aranzada" Rev Cubana Estomctol. 27. 119-127 (1990)

ISHIKAWA,I：“Diversidad en la nepueuta del hmeopeden pacicnts con periodontitis aranzada”Rev Cubana Estomctol。