Function of cell adhesion molecules and cytokine in the periodontal disease.

细胞粘附分子和细胞因子在牙周病中的作用。

• 批准号: 07457453
• 负责人: WATANABE Hisashi
• 金额: $ 5.06万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457453/
• 关键词: clonal anergy; Th2 cytokine; COX-2; human periodontal ligament cell; ALP gnen; T cellクローン; CTLL2細胞; IL2; IFN-γ; 歯周疾患; IL-4; IL-13; 歯周組織; PGE2; 歯周病; 細胞接着分子; サイトカイン; 歯周病原性細菌

T cell clones across an IFN-gamma endothelial cell (EC) barrier in vitro. Transmigration was greatly enhanced after stimulation by Aa and antigen presenting cells (APC). Other stimuli (Con A,IL-2) did not enhance transmigration. Blocking of a second signal provided by APC (with CTLA4-Ig) significantly reduced transmigration. Also, blocking of the adhesion melecule LFA-1 on Th1 or Th2 clones with monoclonal antibody resulted in 75-97% obstruction of transmigration. IFN-gamma treatment of EC resulted in dramatic upregulation of class II MHC expression suggesting that EC could present antigen. IFN-gamma significantly increased Th1 clone transmigration. IL-4 decreased Th1 trasmigration, but enhanced Th2 transmigration. Anergy of transmigrated antigen-specific T lymphocytes can be a protective mechanism that limits extensive Th1 cell proliferation to bacterial antigens and the resultant production of potentially destructive IFN-gamma and IL-2. Transmigration anergy may also play a role in peripheral Tcell tolerance by limiting proliferation of autoreactive gingival T cells. The purpose of the second study was to investigate the involvement of cyclooxygease-1 (COX-1) and cyclooxygenase-2 (COX-2) in prostaglandin (PG) production by human periodontal ligament (PDL) fibroblasts stimulated with a proinflammatory cytokine, interleukin-1beta (IL-1beta), and to examine the effect of interleukin-4 (IL-4), a Th2 cytokine, and interferon-g (IFN-gamma)、a Th1 cytokine, on PG production by the cells. This study suggests that COX-2 are responsible for PEG_2 production by IL-1beta-stimulated human PDL cells and that IL-4 anmd IFN-gamma down-regulate PEG_2 production through inhibition of COX-2 expression and with no effect on COX-2 expression. We also have obtained several results as to ALP gene.

体外T细胞克隆穿过IFN-γ内皮细胞（EC）屏障。Aa和抗原提呈细胞（APC）刺激后，细胞的迁移能力明显增强。其他刺激（刀豆蛋白A，IL-2）并没有增强transmigration。阻断由APC（用CTLA 4-IG）提供的第二信号显著减少了迁移。此外，用单克隆抗体阻断Th 1或Th 2克隆上的粘附分子LFA-1导致75-97%的迁移阻塞。IFN-γ处理EC导致II类MHC表达显着上调，表明EC可以提呈抗原。IFN-γ显著增加Th 1克隆的迁移。IL-4降低Th 1细胞的迁移率，但增强Th 2细胞的迁移率。迁移的抗原特异性T淋巴细胞的无反应性可能是一种保护性机制，其限制了Th 1细胞对细菌抗原的广泛增殖以及潜在破坏性IFN-γ和IL-2的产生。通过限制自身反应性牙龈T细胞的增殖，牙龈迁移无反应性也可能在外周T细胞耐受中发挥作用。第二项研究的目的是研究环氧化酶-1（考克斯-1）和环氧化酶-2（考克斯-2）在促炎细胞因子白细胞介素-1 β刺激的人牙周膜（PDL）成纤维细胞产生前列腺素（PG）中的作用（IL-1 β），并检测Th 2细胞因子白细胞介素-4（IL-4）和Th 1细胞因子干扰素-g（IFN-γ）对细胞产生PG的影响。IL-4和IFN-γ通过抑制考克斯-2的表达而下调IL-1 β刺激的人PDL细胞产生PEG_2的能力，但对考克斯-2的表达无影响。关于ALP基因的研究也取得了一些结果。

项目成果

Watanabe H et al.: "Clinical assessments of the erbium : YAG laser for soft tissue surgery and scaling" J Clin Laser Med Surg. 14. 67-75 (1996)

Watanabe H 等人：“铒的临床评估：YAG 激光用于软组织手术和洁治”J Clin Laser Med Surg。

Ando Y et al.: "Bactericidal effect of erbium : YAG laser on periodontopathic bacteria" Lasers Surg Med. 19. 190-200 (1996)

Ando Y 等人：“铒的杀菌作用：YAG 激光对牙周病细菌的作用”Lasers Surg Med。

Watanabe H et al.: "Wound healing after irradiation of bone tissue by Eri YAG laser." SPIE. 2973. 39-42 (1997)

Watanabe H 等人：“Eri YAG 激光照射骨组织后伤口愈合。”

Goseku-Sone M. Watanabe H et al.: "Hypophosphatasia:Identificaton of five novel miscense mutation in the tissue-nonspecific alkaline phosphe tase gene amang Japanese" Human Mutation. Suppl.1. S263-S267 (1998)

Goseku-Sone M. Watanabe H 等人：“低磷酸酯酶症：在日本人突变中组织非特异性碱性磷酸酶基因中五种新的杂合突变的鉴定”。

Horibe M: "Effect of Perodontal Treatments on Seoum IgG Antibody Titers agoinst Periodontopathic Bacteria" J Clin Periodontol. 22. 510-515 (1995)

Horibe M：“牙周治疗对牙周病细菌 Seoum IgG 抗体滴度的影响”J Clin periodontol。