Cell Adhesion Molecule Expression in Human Periodatitis

人牙周炎细胞粘附分子的表达

• 批准号: 05671591
• 负责人: WATANABE Hisashi
• 金额: $ 1.41万
• 依托单位: Tokyo Medical and Dental University (TMDU)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671591/
• 关键词: Cesll Adhesion Molecule; ICAM-1; VCAM-1; LFA-1; Periodontal Disease; Serum Antibody Titer; サイトカイン; 歯周病原性細菌; 成人性歯周炎; 歯周炎; 接着分子; ELAM-1; RT-PCR法; IL-1β; mRNA

Chronic inflammatory diseases caused by bacterial infection, including adult periodontitis (AP), are characterized by a local accumulation of activated inflammatory cells. We examined whether intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin (endothelial leukocyte adhesion molecule-1 ; ELAM-1) are involved in AP lesions. Gingival tissues from AP patients showed increased levels of these molecules mRNA,compared with those form normal healthy donors. Immunohistological analysis revealed that ICAM-1 was also expressed by gingival fibroblasts. Therefore, we investigated the expression and regulation of ICAM-1 on cultured human gingival fibroblasts (HGF). Cell surface ICAM-1 was upregulated on HGF under transcriptional control by exposure not only to IL-1b, TNF-a, or IFN-g but also to sonic extracts (SE) from periodontopathic bacteria (Porphromonas gingivalis and Prevotella intermedia) and LPS from Escherichia coli. However, these stimuli induced only minimal expression of VCAM-1 and E-selectinon HGF.Finally, binding assays using HGF and Molt4 cells showed induced these molecules to be functional, and the increased binding was blocked by a combination of mAb against ICAM-1 and leukocyte function-associated molecule-1 (LFA-1). Thus, the overexpression of ICAM-1 on gingival fibroblasts induced by cytokines and periodontopathic bacteria is speculated to be deeply involved in the retention and activation of LFA-1 bearing leukocytes in inflamed gingiva.

由细菌感染引起的慢性炎性疾病，包括成人牙周炎（AP），其特征在于活化的炎性细胞的局部积聚。我们研究了细胞间粘附分子-1（ICAM-1）、血管细胞粘附分子-1（VCAM-1）和E-选择素（内皮白细胞粘附分子-1（ELAM-1）是否参与AP病变。牙龈组织从AP患者表现出这些分子的mRNA水平增加，与那些形式正常的健康捐助者。免疫组化分析显示牙龈成纤维细胞也表达ICAM-1。因此，我们研究了ICAM-1在培养的人牙龈成纤维细胞（HGF）上的表达和调节。细胞表面ICAM-1在转录控制下在HGF上上调，不仅暴露于IL-1b、TNF-α或IFN-g，而且暴露于牙周病细菌（牙龈卟啉单胞菌和中间普雷沃氏菌）的声波提取物（SE）和大肠杆菌的LPS。最后，用HGF和Molt 4细胞进行的结合试验显示，这些分子被诱导为功能性的，并且增加的结合被ICAM-1和白细胞功能相关分子-1（LFA-1）的组合阻断。因此，推测细胞因子和牙周病细菌诱导的牙龈成纤维细胞上ICAM-1的过表达深深地参与了炎症牙龈中携带LFA-1的白细胞的保留和激活。

项目成果

Horibe, M., Watanabe, H.and Ishikawa, I.: "Effect of periodontal treatments on serum lgG antibody titers against periodontopathic bacteria." J Clin Periodontol. (in press).

Horibe, M.、Watanabe, H. 和 Ishikawa, I.：“牙周治疗对针对牙周病细菌的血清 IgG 抗体滴度的影响”。

Kubota K,Holli st NO,Olvsile AO,Yonemitu M,Minakuchi S,Watanabe H,et al: "Joint Epidemiclogical Longitudinal Dental Surrey in Nigeria,Especially in Comparison with that of Japanese" Bulletin of Tokyo Medical and Dental University. 40. 59-78 (1993)

Kubota K，Holli st NO，Olvsile AO，Yonemitu M，Minakuchi S，Watanabe H，等：“尼日利亚联合流行病学纵向牙科萨里，特别是与日本的比较”东京医科齿科大学通报。

Watanabe H,Umeda M,Ishikawa I: "Clinical and Laboratory Studies of Severe Periodontal Disease in an Adolescent Associated with Hypophosphatasia" Journal of Periodontology. 64. 174-180 (1993)

Watanabe H、Umeda M、Ishikawa I：“与低磷酸酯酶症相关的青少年严重牙周病的临床和实验室研究”牙周病学杂志。

Motoo Horibe: "Effect of periodontal treatments on serum IgG antibody against periodontopathic bacteria" Journal of Climical Periodontology. (in press). (1995)

Motoo Horibe：“牙周治疗对针对牙周病细菌的血清 IgG 抗体的影响”《临床牙周病学杂志》。

Akira Aoki: "In vifro Studies on Laser Scaling of Subgingival Calcurus with an Erbium:YAG raser" Journal of Periodontology. 65. 1097-1106 (1994)

Akira Aoki：“用铒：YAG 激光器激光去除龈下结石的体外研究”牙周病学杂志。