A Study of Anaerobic Bacteria from the Patients with Nasopharyngeal Cancer or Lung Cancer in Hohg Kong

众所周知，香港的鼻咽癌和女性肺癌的发病率很高，尽管香港女性吸烟者的比例低于日本，或者EB病毒感染率很高，香港和日本几乎相同。我们观察了5例鼻咽癌患者鼻咽部细菌蛋白激酶C（PKC）活性，并与对照组比较。将细菌在有氧和厌氧条件下于37 ° C培养48小时。在需氧和厌氧条件下培养的患者细菌的平均蛋白激酶C活性分别为20.0和13.2mU/mug。对照组在需氧和厌氧条件下培养的细菌的平均活性分别为5.18和10.4mU/mug。我们还从三名香港妇女中收集了慢性咳嗽和咳痰的痰，并在37 ℃下在需氧和厌氧条件下培养了48小时。在有氧条件下培养的细菌没有蛋白激酶活性，这是冈田酸等启动子所显示的。然而，在厌氧条件下培养的两名妇女的细菌显示出强烈的蛋白激酶活性。这些发现表明，可以在厌氧条件下生长的细菌可能在癌症的病因学中起着促进作用。经鉴定为血链球菌。

It is well known that the incidence rates of nasopharyngeal cancer and female lung cancer in Hong Kong, although proportion of female smokers in Hong Kong is less than that in Japan or infection rate of EB virus is high and almost the same in Hong Kong and Japan. There may be other modifing factors like promoters specific to those cancer in Hong Kong.We observed protein kinase C activity of bacteria from nasopharynx of the 5 patients with nasopharyngeal cancer and their controls. The bacteria were cultivated in 37^0C for 48 hours in aerobic and anaerobic conditions. The mean protein kinase C activity for bacteria cultivated in aerobic and anaerobic conditions from the patients was 20.0 and 13.2mU/mug. The mean activity for bacteria cultivated in aerobic and anaerobic condition from the controols was 5.18 and 10.4mU/mug.We also obtained sputum from three Hong Kong women who complain chronic cough and sputum, and cultivated the materials in 37^0C for 48 hours in aerobic and anaerobic conditions. The bacteria cultivated in aerobic condition had no protein kinase activity, which the promoter like Okadaic acid shows. However, the bacteria cultivated in anaerobic condition from the two women showed strong protein kinase activity. These findings suggest that bacteria which can grow in anaerobic condition may have some role in the etiology of cancer as a promoter. The bacteria was identified as Streptococcus sanguis.