Comparative study of cytogenetic and molecular findings in lymphoid malignacies among Japan, USA, China and India

日本、美国、中国和印度淋巴恶性肿瘤细胞遗传学和分子学结果的比较研究

• 批准号: 02042005
• 负责人: KAMADA Nanao
• 金额: $ 8.32万
• 依托单位: Research Institute for Nuclear Medicine and Biology, Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02042005/
• 关键词: Non-Hodgkin's lymphoma; Chronic lymphocytic leukemia; Chromosome analysis; Molecular genetic analysis; PCR; India; 悪性リンパ腫; DNA抽出法

The incidence of non-Hodgkin's lymphoma(NHL) in Japan is one-half of that in the USA. The present study was under taken to understand the incidence of follicular lymphoma and chronic lymphocyte leukemia in Japan and India in comparison to the data from the western countries, and also to investigate among these countries in cytogenetic and molecular genetic findings.Comparative study of follicular lymphoma1. The percentage of follicular lymphoma among the malignant lymphoma seemed to be about 10 in India and Japan, which is very low, comparing to that of USA.2. Six out of 115(40%) Japanese cases with follicular lymphoma showed 14 ; 18 translocation, none of four Indian cases. The percentage of follicular lymphoma cases with the translocation was also very low, comparing to that of USA (85%).3. BCL-2 rearrangement was observed in 5 of 11 (45%) Japanese cases with follicular lymphoma by Southern blot tiny method and 11.1% in Japanese cases and 9.7% in Indian cases by PCR method from the D … More NA of paraffin block. Again, these incidence in both countries were very low, comparing to that of USA.Comparative study of chronic lymphocytic leukemia (CLL)1. Cytogenetic abnormalities were found in 23 of 48 (47.9%) Japanese CLL cases. Trisomy 12 was found in only 17.4 of the cases analyze. These low incidences were completely different from that of USA. Ten Indian and 5 Chinese cases were under examination for chromosome analysis.2. The rearrangement of BCL-1, BCL-2 and BCL-3 were investigated in 55 Japanese CLL cases, of which one case each with BCL-1 and BCL-3 rearrangement and 2 with BCL-2 rearrangements were observed. The two cases with BCL-2 rearrangement had t(2 ; 18)(p12 ; q21) and t(18 ; 22)(q21 ;11), respectively. The former showed rearrangement between IgLK chain and BCL-2 gene in manner of gead to tail joining, the latter showed IgLlambda chain and BCL-2 gene in a manner of head to head joining. Sixteen cases from India and 5 from China are under investigation for molecular genetic analysis. Less

非霍奇金淋巴瘤（NHL）在日本的发病率是美国的一半。本研究旨在了解日本和印度滤泡性淋巴瘤和慢性淋巴细胞白血病的发病率，并与西方国家的数据进行比较，同时调查这些国家在细胞遗传学和分子遗传学方面的发现。滤泡性淋巴瘤的比较研究。在印度和日本，滤泡性淋巴瘤占恶性淋巴瘤的比例约为10%，与美国相比是非常低的。日本115例滤泡性淋巴瘤患者中有6例（40%）显示14；18例易位，4例印度病例中没有一例。与美国（85%）相比，滤泡性淋巴瘤易位的比例也很低。用Southern blot方法检测11例日本滤泡性淋巴瘤中有5例（45%）出现BCL-2重排，用PCR方法检测日本和印度分别有11.1%和9.7%的患者出现BCL-2重排。与美国相比，这两个国家的发病率都很低。慢性淋巴细胞白血病（CLL）的比较研究48例日本CLL病例中有23例（47.9%）发现细胞遗传学异常。在分析的病例中，只有17.4例发现了12型三体。这些低发病率与美国完全不同。对10例印度病例和5例中国病例进行染色体分析。本文对55例日本CLL患者进行了BCL-1、BCL-2和BCL-3重排的研究，其中BCL-1和BCL-3重排各1例，BCL-2重排2例。2例BCL-2重排分别为t(2; 18)（p12; q21）和t(18; 22)（q21;11）。前者显示IgLK链与BCL-2基因以头尾连接方式重排，后者显示IgLlambda链与BCL-2基因以头尾连接方式重排。来自印度的16例病例和来自中国的5例病例正在接受分子遗传分析调查。少

项目成果

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