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Comparative study on lymphoid malignancy between Jap。 & China -A cytogenetic and molecular biological study -

日本与日本淋巴恶性肿瘤的比较研究

基本信息

批准号：
05042004
负责人：
KAMADA Nanao
金额：
$ 2.05万
依托单位：
Research Institute for Nuclear Medicine & Biology, Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1993
资助国家：
日本
起止时间：
1993 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05042004/
关键词：
Non-Hodgkin lymphoma Chronic lymphocytic leukemia Chromosome analysis Molecular genetic analysis PCR India FISH

项目摘要

The incidence of non-Hodgkin's lymphoma (NHL) in Japan is one-half of that in the USA. The present study was under taken to understand the incidence of follicular lymphoma and chronic lymphocyte leukemia in Japan and India in comparison to the data from the western countries, and also to investigate the differences among these countries in cytogenetic and molecular genetic findings.1)Cytogenetic analysis of 73 Japanese NHL cases revealed a lower incidence of 14 ; 18 translocation among follicular lymphoma (40%), compared with the incidence in American cases (75-88%). Japanese CLL cases had almost the same incidence of karyotype abnormalities (45.8%, 51.5%, in USA) and types of abnormalities as American cases (trisomy 12, 14q and 13q abnormalities).However, a higher incidence of complex karyotype was found in Japanese cases than that of western countries (p<0.05).2)The incidence of BCL-2 rearrangement in Japanese/Indian follicular lymphoma was less than that of American cases (45.2% vs 90% USA cases in reports) by Southern blotting, 10.9% vs 53.4% USA cases by PCR. The incidence of BCL-2 rearrangement was not different between that of Japanese and American CLL cases (5.7% vs 6.8-9.3%).3)Detection of trisomy 12 and RB gene loss by means of FISH method in CLL cases revealed that American and Indian cases had a higher incidence of trisomy 12 than that of Japanese cases (30-42.9% vs 19%), whereas RB gene loss of Indian cases (39%) was not significantly different from Japanese cases (28.6%). In general, Indian cases showed almost the same biological characteristics of Japanese B-cell malignancies, although there exists some differences with the American and European cases.

日本非霍奇金淋巴瘤（NHL）的发病率是美国的一半。本研究旨在了解日本和印度的滤泡性淋巴瘤和慢性淋巴细胞白血病的发病率，并与西方国家的数据进行比较，同时研究这些国家在细胞遗传学和分子遗传学方面的差异。1）73例日本NHL病例的细胞遗传学分析显示，14例日本NHL发病率较低;滤泡性淋巴瘤中的18易位（40%），而美国病例的发病率（75-88%）。日本CLL病例的核型异常发生率几乎相同（45.8%，51.5%，在美国）和异常类型作为美国病例（12三体、14 q和13 q异常）。然而，日本病例的复杂核型发生率高于西方国家日本/印度滤泡性淋巴瘤中BCL-2重排的发生率低于美国滤泡性淋巴瘤（P<0.05 Southern杂交检测阳性率为45.2%，美国为90%; PCR检测阳性率为10.9%，美国为53.4%。BCL-2基因重排的发生率在日本和美国的CLL病例中无差异（5.7%vs6.8 -9.3%）。3）用FISH方法检测CLL病例的12三体和RB基因丢失，发现美国和印度病例的12三体发生率高于日本病例（30-42.9% vs 19%），而印度病例的RB基因缺失（39%）与日本病例（28.6%）无显著差异。总体而言，印度病例显示出与日本B细胞恶性肿瘤几乎相同的生物学特征，尽管与美国和欧洲病例存在一些差异。