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Cytogenetic and molecular genetic studies on the atomic-bomb survivors

原子弹爆炸幸存者的细胞遗传学和分子遗传学研究

基本信息

批准号：
09680524
负责人：
KAMADA Nanao
金额：
$ 1.66万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680524/
关键词：
Heavily exposed A-bomb survivors Neoplasms FISH WT-1 Meningioma

项目摘要

Cytogenetic and molecular genetic studies were carried out on the atomic-bomb survivors who had been exposed within 500 meters from the hypocenter.The main results obtained in the past two years1. Follow up of the heavily exposed A-bomb survivors Annual follow up of the exposed people revealed two death of colon cancers. Three survivors were newly found to have cancers, one bladder cancer and two colon cancers.2. A high incidence of meningioma was found among heavily exposed groupThirty eight meningioma patients in exposed group as well as 607 patients in non-exposed group were collected from the database of Hiroshima Tumor Registration. The group exposed more than one gray had significantly higher incidence of meningioma. The incidence were correlated with radiation dose exposed.3. WT1 gene product in the peripheral blood of healthy survivorsTen blood samples were available from the survivors exposed within 500 meters from the hypocenter. Of the ten, four showed a moderate to high level of messenger RNA of the gene 2 within normal level and the remainings un-measurable. No dose-dependency was observed.4. Detection of subclones by FISH techniqueEight leukemia patients who had been exposed more than 2 Gy were examined by FISH technique comparing with the karyotype analyzed by G-banding method. Of the eight patients six were shown to have abnormal subclones either with monosomy 7, 1 7p- and 20q-. Amplification of MYC or p53 genes were also found in four patients among the eight leukemia patients.

对距震源500米以内的原子弹爆炸幸存者进行了细胞遗传学和分子遗传学研究。近两年取得的主要成果1。对严重遭受原子弹爆炸幸存者的跟踪对原子弹爆炸幸存者进行的年度跟踪显示，有两人死于结肠癌。新发现三名幸存者患有癌症，其中一名膀胱癌和两名结肠癌。 2．重度暴露组脑膜瘤发病率较高。从广岛肿瘤登记数据库中收集了暴露组38例脑膜瘤患者和非暴露组607例脑膜瘤患者。暴露超过一灰的人群脑膜瘤发生率明显较高。其发生率与受辐射剂量有关。3．健康幸存者外周血中的 WT1 基因产物从距震源 500 米范围内暴露的幸存者中获得了 10 份血液样本。在这十个中，有四个显示出基因2的信使RNA在正常水平内的中度至高水平，其余的则无法测量。未观察到剂量依赖性。4． FISH技术检测亚克隆对8例照射剂量超过2Gy的白血病患者进行FISH技术检查，并与G显带法分析核型进行比较。在这 8 名患者中，有 6 名被证明具有异常亚克隆，其中有 7 号单体、1 号 7p- 和 20q- 单体。 8名白血病患者中的4名患者也发现了MYC或p53基因的扩增。