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Molecular and cytogenetic studies on hematopoietic cells in heavily exposed A-bomb survivors

重度原子弹爆炸幸存者造血细胞的分子和细胞遗传学研究

基本信息

批准号：
01480534
负责人：
KAMADA Nanao
金额：
$ 4.16万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

I. Studies on heavily exposed A-bomb survivors who have no clinical trouble at present ;a)Cytogenetic studies revealed 5- 45.6% of chromosome abnormalities in the peripheral lymphocytes, which were. estimated to be 9- 650 rad of exposure at the time of bombing.b)Serological studies for anti-BAST antibody shown that percent of position cases was significantly higher in the proximally exposed group.c)Molecular-biological studies revealed the transforming -N-ras and K-ras genes in two and one survivors, respectively.II. Studies on leukemia patients with a history of exposed to Atomic Bomb Radiation.a)Cytogenetic study on acute leukemia :Seventy five radiation--'related leukemia(acute non-lymphocyte)in Hiroshima including 16 patients exposed to more than one Gray were cytogenetically examined. Statistical analysis of the data on the frequencies of chromosomal aberrations in survivors according to the bone marrow doses of DS86 estimation revealed that heavily exposed patients tended to have significantly higher aberration rates as compared, -with non-exposed patients. Furthermore, the chromosomal aberrations in the survivors were-observed to be of a more complex nature and had characteristic findings of secondary leukemia.b)Molecular biological studies on transforming genes in acute and chronic leukemia and the bcr gene in chronic myelocytic leukemia have been performed in exposed and non-exposed groups. So far, no distinctive differences have been observed in the frequency and the sites of point mutations in N-. and K-ras genes or in the rearrangement of the bcr gene, for a final conclusion of the specificity of radiation induced leukemia.

I.对目前无临床症状的严重暴露原子弹幸存者的研究：a）细胞遗传学研究显示，外周淋巴细胞染色体异常率为5- 45.6%，B）抗BAST抗体的血清学研究表明，在近距离暴露组中，位置病例的百分比显著较高。急性白血病的细胞遗传学研究：对广岛75例放射相关白血病（急性非淋巴细胞白血病），包括16例受一种以上Gray照射的患者进行了细胞遗传学检查。根据DS 86骨髓剂量估计值，对幸存者染色体畸变频率数据进行统计分析，结果显示，与未暴露患者相比，重度暴露患者的畸变率往往显著更高。此外，观察到幸存者的染色体畸变具有更复杂的性质，并具有继发性白血病的特征性发现。B）在暴露组和非暴露组中对急、慢性白血病的转化基因和慢性粒细胞白血病的bcr基因进行了分子生物学研究。到目前为止，没有观察到明显的差异，在N-的点突变的频率和位点。和K-ras基因或bcr基因重排的关系，以最终得出放射性白血病的特异性结论。