Quantum Theory and Spectroscopy of Proteins as Biomachines

作为生物机器的蛋白质的量子理论和光谱学

• 批准号: 02305013
• 负责人: KITAGAWA Teizo
• 金额: $ 7.04万
• 依托单位: Okazaki National Research Institutes, Institute for Molecular Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02305013/
• 关键词: Biomachine; Proteins; Spectroscopy; MO calculation; Protein dynamics; 量子生物学; 生体分子分光学; 時間分解分光学

This cooperative research aims cooperation of theoreticians in basic chemistry and biophysics with experimentalists in biochemistry and spectroscopy. The two kinds of scientists do not see each other in ordinary scientific meetings. In order to create a new concept about proteins which work as biomachines, it is desirable to prepare opportunities for the two kinds of scientists to discuss. Accordingly, we had three meetings during two years. The main subjects are electron and energy transfer in proteins, ab inito MO calculations for large molecules, time-resolved spectroscopy of biological molecules, and dynamics of proteins. Materials treated by the members include various heme proteins, photosynthetic reaction centers, retinoid proteins, various membrane proteins and enzymes. Practical activities and results are published in a separate booklet.

这项合作研究旨在基础化学和生物物理学的理论家与生物化学和光谱学的实验家的合作。这两种科学家在普通的科学会议上不会见面。为了创建关于作为生物机器的蛋白质的新概念，需要为两种科学家的讨论准备机会。因此，我们在两年内召开了三次会议。主要科目是蛋白质中的电子和能量转移、大分子的从头开始的MO计算、生物分子的时间分辨光谱以及蛋白质动力学。成员处理的物质包括各种血红素蛋白、光合反应中心、类维生素A蛋白、各种膜蛋白和酶。实践活动和结果发表在单独的小册子中。

项目成果

Y.Mizutani,S.Tokitomi,K.Aoyagi,K.Horitsu,T.Kitagawa: "Resonance Raman study on intact pea phytochrome and its model compounds;Evidence for proton migration during the phototransformation" Biochemistry. 30. 10693-10700 (1991)

Y.Mizutani，S.Tokitomi，K.Aoyagi，K.Horitsu，T.Kitakawa：“完整豌豆光敏色素及其模型化合物的共振拉曼研究；光转化过程中质子迁移的证据”生物化学。

V. Fidler, T. Ogura, S. Sato, K. Aoyagi, T. Kitagawa: "Resonance Raman study on photoreduction of iron-porphyrins : a novel insigh into the ligandaided process" Bull. Chem. Soc. Jpn.64. 2315-2322 (1991)

V. Fidler、T. Ogura、S. Sato、K. Aoyagi、T. Kitakawa：“铁卟啉光还原的共振拉曼研究：对配体辅助过程的新颖洞察”公牛。

Y. Mizutani, S. Hashimoto, Y. Tatsuno, T. Kitagawa: "Resonance Raman pursuit of the change from Fe^<II>-O_2 to Fe^<III>-OH via Fe^<IV>=O in autoxidation of ferrous iron-porphyrin" J. Am. Chem. Soc.112. 6809-6814 (1990)

Y. Mizutani、S. Hashimoto、Y. Tatsuno、T. Kitakawa：“共振拉曼追踪在自氧化过程中通过 Fe^<IV>=O 从 Fe^<II>-O_2 到 Fe^<III>-OH 的变化

N. Kitajima, T. Koda, S. Hashimoto, T. Kitagawa, Y. Moro-oka: "Synthesis and characterization of the dinuclear copper (II) complexes [Cu (HB (3, 5-Me_2pz)_3) ]_2 X (X=O^<2->, (OH)_2^<2->, CO_3^<2->, O_2^<2->)" J. Am. Chem. Soc.113. 5664-5671 (1991)

N. Kitajima、T. Koda、S. Hashimoto、T. Kitakawa、Y. Moro-oka：“双核铜 (II) 配合物 [Cu (HB (3, 5-Me_2pz)_3) ]_2 X 的合成和表征

T. Egawa, Y. Imai, T. Ogura, T. Kitagawa: "Resonance Raman study on mutant cytochrome P-450 obtained by site directed mutagenesis" Biochim. Biophys. Acta. 1040. 211-216 (1990)

T. Ekawa、Y. Imai、T. Ogura、T. Kitakawa：“通过定点诱变获得的突变细胞色素 P-450 的共振拉曼研究”Biochim。