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植物の温度検知機構の分子生物学的解析

植物温度传感机制的分子生物学分析

基本信息

批准号：
02404004
负责人：
MURATA Norio
金额：
$ 3.14万
依托单位：
National Institute for Basic Biology
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02404004/
关键词：
Temperature sensing Desaturase Cyanobacterium Temperature-induced regulation of gene expression

项目摘要

1. Purpose: When plants are exposed to low-temperature, they introduce double bonds into fatty acids of membrane lipids, which compensates the decrease in molecular motion of membranes caused by low temperature. This phenomenon suggests that the plants can detect the change in temperature and transduce this signal to the expression of desaturase genes. The purpose of this research is to study the mode of temperature-induced regulation of desaturase expression in cyanobacteria.2. Results:(1) Genes (desA) for plant-type desaturases at the DELTA12 position were cloned from Synechocystis PCC6714 and Synechococcus PCC7002 with a probe derived from desA of Synechocystis PCC6803. When Synechococcus PCC7914, which is incapable of the DELTA12 desaturation, was transformed with these desA genes, this strain acquired the ability to introduce the double bond at the DELTA12 position. These observations confirm that the cloned genes were all desA.(2) The northern blot analysis of desA gene expression in Synechocystis PCC6803 demonstrated that the transcription of desA gene was markedly enhanced upon decrease in temperature.(3) A Promoter-detecting cassette constructed from the plasmid pBSDELTAAv was introduced into the genome of Synechocystis PCC6803. In a number of mutants produced some responded to temperature changes We are now identifying the temperature-responsible promoters.

1.目的：当植物暴露于低温时，它们将双键引入膜脂的脂肪酸中，从而补偿低温引起的膜分子运动的降低。这种现象表明植物可以检测温度的变化并将该信号传递给去饱和酶基因的表达。本研究的目的是研究温度诱导蓝藻脱氢酶表达的调控模式.结果：（1）以来自集胞藻6803的德萨为探针，从集胞藻6714和集胞藻7002中克隆到植物型去饱和酶DELTA 12位点的基因（德萨）。当用这些德萨基因转化不能进行DELTA 12去饱和的聚球藻PCC 7914时，该菌株获得了在DELTA 12位置引入双键的能力。这些观察结果证实克隆的基因都是德萨。(2)对集胞藻PCC6803中desA基因表达的北方杂交分析表明，德萨基因的转录随温度的降低而显著增强。(3)将由质粒pBSDELTAAv构建的启动子检测盒导入集胞藻PCC 6803的基因组中。在一些突变体中产生了一些对温度变化有反应的突变体，我们现在正在鉴定对温度有反应的启动子。