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Genetic engineering of temperature stress tolerance

温度胁迫耐受性基因工程

基本信息

批准号：
06044233
负责人：
MURATA Norio
金额：
$ 3.39万
依托单位：
Okazaki National Research Institute
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Dr.Gombos stayd in the National Institute for Basic Biology from September 6th to December 1st, 1995.He, together with Dr.Murata and Dr.Hayashi, studied the photoinhibition of photosystem II at low temperature in Synechocystis sp.PCC 6803.The Synechocystis cells were exposed to various periods of strong light to induce the photoinhibition, which was followed by the recovery of the photosynthetic machinery from the photoinhibited state under dim light at room temperature.It was demonstrated that the photoinhibition was separated into two components ; one was reversible, and the other was irreversible.The reversible component can be explained by the turnover of the D1 protein in the photosystem II complex.The irreversible component had not been well studied, and its molecular mechanism is unknown.In the view point of physiology, the irreversible photoinhibition is deteriorious to the photosynthetic organisms, whereas the reversible photoinhibition provides them with temporal depression of photosynthesis.2.Dr.Okuyama stayd in Biological Research Center from July 14th to July 29th, 1995.He, together with Dr.Gombos and Dr.Szalontai, studied the cryophilicity and the physical phase of membrane lipids of antarctic Haptophyte, Prymnesiophyte strain B.The photosynthesis of the cells of this strain grown 5ﾟC revealed the optimum temperature at 5ﾟC,and the phase transition of the membrane lipids3.Dr.Murata stayd in Biological Research Center from March 10th to March 29th, 1996.He, together with Dr.Gombos and Dr.Hideg, studied the heat tolerance of Synechococcus sp.PCC 7002.The disruption the groEL-beta gene by insertional mutation decreased the viability of this strain after the heat shock.However, the disruption did not affect the heat stability of the photosynthetic activity.These results are likely to suggest that the heat shock protein GroEl-beta is not related to the heat acclimation.appeared below 5ﾟC.

Gombos博士于1995年9月6日至12月1日在国立基础生物学研究所，与Murata博士和Hayashi博士一起研究了集胞藻6803在低温下光系统II的光抑制。结果表明，光抑制分为两部分：一部分是光抑制，另一部分是光抑制。一种是可逆的，另一种是不可逆的，可逆的部分可以用光系统II复合体中D1蛋白的周转来解释，不可逆的部分还没有得到很好的研究，其分子机制还不清楚，从生理学的角度来看，不可逆的光抑制对光合生物是有害的，而可逆的光抑制使它们的光合作用暂时受到抑制。2. Okuyama博士从1995年7月14日至7月29日在生物研究中心工作。他与Gombos博士和Szalontai博士一起，研究了一株无水附着植物Prymnesiophyte B菌株的嗜冷性和膜脂的物理相。该菌株在5 ℃下生长的细胞光合作用的最适温度为5 ℃，膜脂的相转变。3. Murata博士于1996年3月10日至3月29日在生物研究中心逗留。他与Gombos博士和Hideg博士一起，研究了聚球藻7002的耐热性。通过插入突变的方法将GroEL-β基因插入突变体中，使该菌株在热激后的存活率下降，但对光合活性的热稳定性没有影响。这些结果可能表明，热激蛋白GroEL-β与5 ℃以下出现的热驯化无关。