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Sensors of Environmental Stress

环境压力传感器

基本信息

批准号：
14086207
负责人：
MURATA Norio
金额：
$ 41.66万
依托单位：
National Institute for Basic Biology (2004)Okazaki National Research Institutes (2002-2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Histidine kinase (Hik) and response regulator (Rre) constitute the twp-component system that is a signal perception/transduction pathway in prokaryotes and some eukaryotes. The cyanobacterium Synechocystis sp. PCC 6803, which performs oxygenic photosynthesis like plants, contains 47 genes for Hiks and 45 genes for Rres. We mutated these genes successfully, except three genes for Hiks and three genes for Rres, and constructed mutant libraries of Hiks and Rres. By screening the libraries by genome-wide expression of genes under various stress conditions with cDNA microarrays, we obtained the following results.Hyperosmotic stress, due to 0.5 M sorbitol, induced the expression of 246 genes. Screening of the above-mentioned mutant libraries with respect to the hyperosmotic stress-inducible expression of genes by the cDNA microarray method revealed that 60% of the hyperosmotic stress-inducible genes are under control of five two-component systems, namely, Hik33/Rre31, Hik16/Hik41/Rre17, Hik34/Rre1, Hik2/Rre1, and Hik10/Rre3. Remaining 40% of the hyperosmotic stress-inducible genes are under control of as-yet unidentified signal-transducing pathways.Salt stress, due to 0.5 M NaCl, induced the expression of 294 genes. Screening of the above-mentioned mutant libraries with respect to the salt stress-inducible expression of genes by the cDNA microarray method revealed that 70% of the salt stress-inducible genes are under control five two-component systems as in the case of hyperosmotic stress-inducible genes. However, genes under the individual Hik/Rre two-component systems are different between the salt stress and hyperosmotic stress. Remaining 30% of the salt stress-inducible genes are under control of as-yet unidentified signal-transducing pathways.

组氨酸激酶（Hik）和反应调节因子（Rre）组成了原核生物和某些真核生物的双组分信号转导系统。蓝细菌集胞藻PCC 6803，其像植物一样进行产氧光合作用，含有47个Hiks基因和45个Rres基因。除Hiks基因和Rres基因各3个外，其余基因均成功突变，并构建了Hiks和Rres突变体文库。通过用cDNA微阵列筛选在各种胁迫条件下全基因组表达基因的文库，我们获得了以下结果：高渗胁迫，由于0.5 M山梨醇，诱导246个基因的表达。通过cDNA微阵列方法筛选上述突变体文库中高渗胁迫诱导型基因表达，发现60%的高渗胁迫诱导型基因受5个双组分系统控制，即Hik 33/Rre 31、Hik 16/Hik 41/Rre 17、Hik 34/Rre 1、Hik 2/Rre 1和Hik 10/Rre 3。其余40%的高渗胁迫诱导基因的控制下，尚未确定的信号转导途径。盐胁迫，由于0.5 M NaCl，诱导294个基因的表达。通过cDNA微阵列方法筛选上述突变体文库中的盐胁迫诱导型基因表达，发现70%的盐胁迫诱导型基因与高渗胁迫诱导型基因的情况一样处于5个双组分系统的控制下。然而，在盐胁迫和高渗胁迫下，单个Hik/Rre双组分系统下的基因是不同的。其余30%的盐胁迫诱导基因的控制下，尚未确定的信号转导途径。