Studies on low-temperature sensors and the mechanisms of acclimation to low-temperature

低温传感器及其低温适应机制研究

• 批准号: 13854002
• 负责人: MURATA Norio
• 金额: $ 78.96万
• 依托单位: National Institute to Basic Biology (2004)Okazaki National Research Institutes (2001-2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (S)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13854002/
• 关键词: Cold sensor; Histidine kinase; DNA super-coiling; Response regulater; Cyanobacterium; Ser; Thr protein kinases; 遺伝子発現; DNAミクロアレイ; セリン・スレオニン・キナーゼ; DNAマイクロアレイ; 酸化ストレスセンサー; ヒスチジン・キナーゼ; マルチ・ストレス・センサー; 塩センサー; マルチ・チャンネル・センサー; 浸透圧センサー; プロテオーム

(1) The cyanobacterium Synechocystis sp. PCC 6803 contains 44 genes for histidine kinases (Hiks) and 42 genes for response regulators (Rres). We individually inactivated all of these signal transducers and generated knockout libraries. By screening these libraries by cold-inducible expression of genes with genome-wide DNA microarrays we identified Hik33 as only one Hik for cold sensing and Rre 26 and Rre 31 as response regulators located downstreem of Hik33 in the cold signal transduction pathway.(2) We identified Hik33, which we previously identified as a cold sensor, as a component involved in the signal pathways of hyperosmotic, cold, strong-light and oxidative stress. These findings suggest that Hik33 itself is not specific to the individual stress and that the signal transduction pathways which include Hiks and Rres are more complicated than the simple two-component systems which have been established previously. It is very likely that Hik33 cooperates with other factors that give the specificity to individual stress. Identifications of such factors will be an important subject of further study.(3) We demonstrated possibilities that some Ser/Thr protein kinases are involved in the cold-signal transduction pathway and that DNA supercoiling may directly sense the cold signals.

(1)聚胞藻（synnechocystis sp. PCC 6803）含有44个组氨酸激酶（Hiks）基因和42个反应调节因子（Rres）基因。我们单独灭活了所有这些信号换能器并生成了敲除文库。通过使用全基因组DNA微阵列对这些文库进行冷诱导表达筛选，我们发现Hik33是冷感应的唯一Hik， Rre 26和Rre 31是位于Hik33下游的冷信号转导通路的响应调节因子。(2)我们发现Hik33是参与高渗、冷、强光和氧化应激信号通路的一个成分，而Hik33先前被认为是一个冷传感器。这些发现表明，Hik33本身并不是特定于个体应激的，包括Hiks和Rres在内的信号转导途径比之前建立的简单的双组分系统更为复杂。Hik33很可能与其他因素合作，赋予个体压力特异性。确定这些因素将是进一步研究的一个重要课题。(3)我们证明了一些丝氨酸/苏氨酸蛋白激酶参与冷信号转导途径的可能性，并且DNA超卷曲可能直接感知冷信号。

项目成果

Gene expression profiling reflects physiologycal processes in salt acclimation in Synechocystis sp. Strain PCC 6803

基因表达谱反映了集胞藻盐适应的生理过程。

• 发表时间: 2004
• 期刊: Plant Physiology 136
• 作者: K.Marin;Y.Kanesaki;D.A.Los;N.Murata;I.Suzuki;M.Hagemann
• 通讯作者: M.Hagemann

S.Suzuki: "The SphS-SphR two component system is the exclusive sensor for the induction of gene expression in response to phosphate limitation in Synechocystis."Journal of Biological Chemistry. In press. (2004)

S.Suzuki：“SphS-SphR 双组分系统是针对集胞藻中磷酸盐限制诱导基因表达的唯一传感器。”《生物化学杂志》。

K.Mikami: "The histidine kinase Hik33 perceives osmotic stress and cold stress in Synechocystis sp. PCC 6803"Molecular Micro biology. 46. 905-915 (2002)

K.Mikami：“组氨酸激酶 Hik33 感知集胞藻 PCC 6803 中的渗透应激和冷应激”分子微生物学。

Gene expression profiling reflects physiological processes in salt acclimation in Synechocystis sp.Strain PCC 6803

基因表达谱反映了集胞藻菌株 PCC 6803 盐驯化的生理过程

• 发表时间: 2004
• 期刊: Plant Physiology 136
• 作者: K.Marin;Y.Kanesaki;D.A.Los;Murata;I.Suzuki;M.Hagemann
• 通讯作者: M.Hagemann

M.Inaba: "Gene-engineered rigidification of membrane lipids enhances the cold inducibility of gene expression in Synechocystis."Journal of Biological Chemistry. 278. 12191-12198 (2003)

M.Inaba：“基因工程膜脂刚性化增强了集胞藻基因表达的冷诱导能力。”生物化学杂志。