Antioxidative function of phospholipids in polyunsaturated oils and biomembranes

多不饱和油和生物膜中磷脂的抗氧化功能

• 批准号: 02806024
• 负责人: MIYAZAWA Teruo
• 金额: $ 1.02万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02806024/
• 关键词: antioxidant; phospholipid; perilla oil; hexagonal phase; lamellar phase; lipid peroxidation; 抗酸化物質; 高度不飽和油脂; リポソ-ム; 老化; 肝臓癌

Antioxidant effect of phospholipids on the oxidation of refined polyunsaturated oil, such as perilla oil, was investigated by measuring weight-gains and by the oven test. The oxidative stability of perilla oil was especially increased by additions of phosphatidylethanolamine (PE) and phosphatidylserine (PS), but phosphatidylcholine (PC) scarcely showed an antioxidant effect. In these experimental systems, the antioxidant effects of phospholipids, especially of amino-phospholipids such as PE and PS, were due to synergistic function with tocopherols that present in the polyunsaturated oils.The effect of phase behavior on the peroxidation of biomembranes has been investigated for hexagonal II phase and lamellar phase as to explain the antioxidative characteristics of phospholipid molecules, in dilinoleoyl PC and dilinoleoyl PE aqueous dispersions. The results indicated that hexagonal II phase is more sensitive to hydroperoxidation than bilayer phase in phospholipid aqueous dispersions.

通过增重测定和烘箱试验，考察了磷脂对紫苏油等精制多不饱和油脂氧化的影响。添加磷脂酰乙醇胺(PE)和磷脂酰丝氨酸(PS)可显著提高紫苏油的氧化稳定性，而磷脂酰胆碱(PC)几乎没有抗氧化作用。在这些实验体系中，磷脂，特别是氨基磷脂，如PE和PS，其抗氧化作用是由于与多不饱和油脂中存在的生育酚的协同作用。研究了六方相和片状相相行为对生物膜过氧化的影响，以解释磷脂分子在双甘油酰PC和双甘油酰基PE水分散体中的抗氧化特性。结果表明，在磷脂水分散体中，六方II相比双层相对氢过氧化更敏感。

项目成果

JinYe Wang: "Inverted hexagonal phase is more sensitive to hydroperoxidation than multilamellar phase in phosphatidylcholine and phosphatidylethanolamine aqueous dispersions" FEBS Letters.

JinYe Wang：“在磷脂酰胆碱和磷脂酰乙醇胺水分散体中，倒置六角相比多层相对氢过氧化更敏感”FEBS Letters。

JIN YE WANG: "ReverseーHexagonal Phase is more Sensitive to Hydroperoxidation than Multilamellar Phase in Phosphatidylchcline and Phosphatdylethanolamine Aqneous Dispersions" Bioscience Biotechnology and Biochemistry.

金业王：“磷脂酰胆碱和磷脂酰乙醇胺水分散体中的反六角相对氢过氧化反应比多层相更敏感”《生物科学生物技术与生物化学》。

MINORU KASHIMA: "The Antioxidant Eftects of phospholipids on Perilla Oil" Journal of American Oil Chemists'Society. 68. 119-122 (1991)

Minoru KASHIMA：“磷脂对紫苏油的抗氧化作用”美国石油化学家协会杂志。

Lucia Satiko Yoshida: "Liver phosphatidylcholine hydroperoxidation provoked by ethionineーcontaining cholineーdeficient diet in mice" Lipids. 25. 565-569 (1990)

Lucia Satiko Yoshida：“小鼠中含有乙硫氨酸的胆碱缺乏饮食引起的肝脏磷脂酰胆碱氢过氧化”Lipids 25. 565-569 (1990)。

Minoru Kashima: "The antioxidant effects of phospholipids on perilla oil." Journal of American Oil Chemists' Society. 68 (2). 119-122 (1991)

Minoru Kashima：“磷脂对紫苏油的抗氧化作用。”