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Localization and turnover of calcium in biominerals (otoconia, bone and teeth).

生物矿物质（耳石、骨骼和牙齿）中钙的定位和周转。

基本信息

批准号：
02807002
负责人：
KAWAMATA Seiichi
金额：
$ 1.02万
依托单位：
Toyama Medical and Pharmaceutical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

The localization of pyroantimonate-precipitable calcium was examined in calcifying systems. Specimens were fixed by the pyroantimonate method and selectively decalcified by treatment with ammonium chloride. After this treatment, otolithic organs and young bones were easy to cut into sections and following results were obtained:1.The fixation with pyroantimonate and glutaraldehyde (two step method) resulted in better preservation of tissue and more precise localization of precipitaes than did the direct immersion of specimens in pyroantimonate and osmium tetroxide (one step method).2. In the endolymphatic sac of the tree frog, the precipitate was distributed around calcium carbonate crystals. When crystalline growth was accelerated, the precipitate increased in amount. These crystals were considered to grow by accretion.3. In the otolithic organs in the inner ear of the rat and guinea pig, the precipitate was found around otoconia where a silver methenamine-positive material existed.4. In bone of very young rats, the precipitate was frequently observed within the endoplasmic reticulum of obsteoblasts, and on vacuoles and the ruffled border of osteoclasts. The distribution of calcium pyroantimonate in decalcified bone was the same as that in undecalcified counterparts.5. The precipitates of calcium pyroantimonate were well retained and the fine structure of the tissue was well preserved after the treatment with ammonium chloride.6. The presence of calcium in the precipitates was confirmed by X-ray microanalysis and extraction with EGTA.

在钙化系统中检查了焦锑酸盐可沉淀钙的定位。标本通过焦锑酸盐法固定，并通过氯化铵处理选择性脱钙。经此处理后，耳石器官和幼骨易于切片，得到以下结果：1.焦锑酸盐和戊二醛固定（两步法）比直接将标本浸泡在焦锑酸盐和四氧化锇（一步法）中可以更好地保存组织，沉淀物定位更精确。2．在树蛙的内淋巴囊中，沉淀物分布在碳酸钙晶体周围。当晶体生长加速时，沉淀物的量增加。这些晶体被认为是通过吸积生长的。3．大鼠和豚鼠内耳耳石器官中，耳石周围有沉淀物，存在银乌洛托品阳性物质。 4．在非常年幼的大鼠的骨骼中，经常在成骨细胞的内质网内、破骨细胞的液泡和皱边边界上观察到沉淀物。焦锑酸钙在脱钙骨中的分布与未脱钙骨中的分布相同。5．氯化铵处理后，焦锑酸钙沉淀保留良好，组织的精细结构得到很好的保存。 6．通过 X 射线微量分析和 EGTA 萃取证实了沉淀物中钙的存在。