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The analysis of blue light-induction of transport carrier in Chlorella plasmalemma

小球藻质膜转运载体蓝光诱导分析

基本信息

批准号：
02640529
负责人：
KAMIYA Akio
金额：
$ 0.26万
依托单位：
Faculty of Pharmaceutical Sciences, Teikyo Uuiv.
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

In a cell of Chlorella the uptake of exogenous glucose has been found to be reduced under the influence of blue light.The Chlorella cell possesses an inductive uptake system for transmembrane transport of exogenous glucose. Reasonably, this carrier protein is synthesized in the presence of exogenous glucose. It appeared important to examine the influence of light on the behavior of substrate uptake such as glucose, amino acids and several ions. In the following, data on the light effect on several uptake systems in a Chlorella mutant are presented.1. Blue light enhanced amino acid uptake in resting cells.2. The addition of Ca^<2+> enhanced glucose update. Glucose uptake is accompanied with H^+ symport and K^+ efflux, both of which were also enhanced in the presence of exogenous Ca^<2+>.3. The inhibition of H^+ symport and ^+ efflux accompanied with glucose uptake was also observed in mutant cells irradiated with blue light. In the presence of exogenous Ca^<2+>, however, this inhibitory effect on glucose uptake was significantly protected.4. Red and far-red(735 nm) light enhanced ^+ efflux and the latter lingt showed more effective, while blue light slightly enhanced ^+ influx. The ^+ efflux under far-red light is likely to be accompanied by hyperpolarization of the plasmalemma.5. To detect the flow of calcium ion under light we succeeded to load calcium fluorescent probe indo-l to a mutant cell at p_H 4.0.6. The isolation of plasmamembrane in mutant cells is not succeeded in this research project.

小球藻细胞对外源葡萄糖的吸收在蓝光的影响下降低，小球藻细胞具有诱导吸收外源葡萄糖的跨膜转运系统。合理地，该载体蛋白在外源性葡萄糖存在下合成。研究光对葡萄糖、氨基酸和几种离子等底物摄取行为的影响显得很重要。在下文中，呈现了关于小球藻突变体中的几种吸收系统的光效应的数据。蓝光增强静息细胞对氨基酸的摄取. Ca^<2+>的加入促进了葡萄糖的更新。葡萄糖的摄取伴随着H^+的同向转运和K^+的外排，外源Ca^<2+的存在也促进了这两种转运。在蓝光照射的突变体细胞中，还观察到H^+同向转运和^+外排的抑制以及葡萄糖摄取。然而，在外源Ca^<2+>的存在下，这种对葡萄糖吸收的抑制作用得到显着保护。4.红光和远红光（735 nm）可增强^+外排，且后者更有效，而蓝光可略微增强^+内流。在远红光下^+流出可能伴随着质膜的超极化。为了检测光下钙离子的流动，我们成功地将钙荧光探针indo-1负载到pH4.0.6的突变细胞中。本研究项目未能成功分离突变体细胞的质膜。