精子の成熟と受精能獲得に関する研究

精子成熟和生育力获得的研究

• 批准号: 02640549
• 负责人: 毛利 秀雄
• 金额: $ 0.7万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02640549/
• 关键词: 精子の成熟; 受精能獲得; 先体反応; ハイパ-アクチベ-ション; 脂肪酸; ステロ-ル

哺乳類精子は精巣内で形態的にはほぼ完成するが、運動能や受精能はまだ備わっていない。精子は雄性生殖管内で成熟して運動能を獲得し,また射出後雌性生殖管内で受精能獲得を行い,ハイパ-アクチベ-ション(超活性化)や先体反応をおこして卵と受精する。これらの過程での膜系の変化を明らかにするために、ハムスタ-精子を実験材料として脂質の変化を追及した。その結果。脂肪酸組成については、精巣上体尾部精子は同頭部精子に比べて、パルミチン酸の割合いが低くステアリン酸の割合いが高かった。しかし全脂肪酸量に有意な差は認められなかった。次にmーTALPー6溶液中で超活性化をおこした精巣上体尾部精子について脂肪酸を分称したところ,脂肪酸組成に全く変化は認められなかった。しかしCaイオノフォアを用いて先体反応を清起した精子では,哺乳類精子に特徴的な脂肪酸であるドコサヘキサエン酸とドコサペンタエン酸の割合いの減少とパルミチン酸の割合いの増加が認められた。これらのことから,先体反応の過程で脂肪酸組成に大きな変化がおこることがわかった。ところで,精子の成熟過程ではステロ-ルの組成がいちじるしく変化し,精巣上体頭部ではコレステロ-ルが主要ステロ-ルであるが,尾部精子ではコレステロ-ル生合成中間体のデスモステロ-ルやコレスタ-ク,24ージエンー3βーオ-ルが高い割合いを占める。これは△^7ー5ーデサチュラ-ゼが精巣上体尾部では不活性化されているか,あるいは存在しない可能性を示唆する。この点を明らかにするために,この酵素活性の検出法を検討中である。なお除膜精子を用いた運動パタ-ンの解析では,cAMPの濃度上昇および低濃度のCa^<2+>が超活性化されたものと似た運動を示し,中片部が大きく屈曲するようになることがわかった。

The morphology of mammalian spermatozoa completes the sperm cycle, the motility and fertilization ability of the mammalian spermatozoa. The motility of mature spermatozoa in the male reproductive tube was successful. After the sperm was ejected, the fertilization in the female reproductive tube was successful, and the egg was fertilized in the precursors. In the course of the process, the membrane system was used to understand the characteristics of the membrane system, such as sperm, sperm, fat, fat, and so on. Compare the results. The composition of fatty acids, the sperm of the upper body and tail of the sperm, the sperm of the upper body and tail of the sperm, the sperm of the upper body and tail of the sperm, the sperm of the upper body and tail of the sperm, the sperm of the upper body and tail of the sperm, the sperm of the upper body and tail of the sperm, the sperm of the upper body and tail of the sperm, the sperm of the upper body and tail of the sperm, the sperm of the upper body and tail of the sperm, the sperm of the upper body and tail of the sperm, the sperm of the upper body and tail of the sperm, the sperm of the upper body and tail of the sperm, the sperm of the upper body and tail of the semen, the sperm of the upper body and tail of the sperm, the sperm of the upper body and tail of the semen, the sperm of the upper body and tail of the semen There is an intentional difference in the amount of total fatty acid. In the solution of sub-TALP 6, the fatty acids of spermatozoa from the upper body and tail of the spermatozoa were called the fatty acids, and the fatty acid composition of the whole spermatozoa was determined. To clean up the sperm of mammalian spermatozoa, the special fatty acids of mammalian spermatozoa have been used to clean up the spermatozoa of mammalian spermatozoa in the first place. The fatty acids of the mammalian spermatozoa have been detected by the Ca method. In the first place, the fatty acid composition of the process fatty acid is very important. In the process of sperm maturation, the spermatozoa matured, the spermatozoa were formed, the upper body of the spermatozoa, the upper body, the main body, the tail, the tail, the spermatozoa, the spermatozoa The tail of the upper body is inactive, and there is a possibility that there is a possibility of inactivation. Please tell me that the enzyme activity is very high, and the enzyme activity is very high. The spermatozoa were removed from the membranous sperm by the method of motion analysis, and the results of the cAMP test showed that the activity of the sperm was lower than that of the lt;2+>, and that of the sperm in the middle of the film was similar to that in the middle part of the film.

项目成果

Awano,M.,Kawaguchi,A.,Mohri,H.: "Changes in lipids during maturation,capacitation and acrosome reaction of hamster spermatozoa."

Awano,M.、Kawaguchi,A.、Mohri,H.：“仓鼠精子成熟、获能和顶体反应过程中脂质的变化。”

Ishijima,S.,Mohri,H.: "Beating patterns of mammalian spermatozoa.In“Controls of Sperm Motility:Biological and Clinical Aspects"" CRC Press, 29-42 (1990)

Ishijima, S., Mohri, H.：“哺乳动物精子的跳动模式。在“精子活力的控制：生物学和临床方面””CRC Press，29-42 (1990)