The Improvement of Efficiency on the Microdissection in the SEM using Compound Materials : Its Development and Practice.

“利用复合材料提高扫描电镜显微解剖效率：开发与实践”的研究经费在1991年拨款发放后立即开始。首先，用我们改进的碳化钨针制作了显微解剖尖端仪器。它们被电解削尖并用于显微解剖。同时开始了新型金属纤维复合材料尖端仪器的试制工作。纤维材料碳纤维和碳化硅纤维由日本东北大学钢铁及其他材料研究所、东洋株式会社、日本炭素株式会社提供。最后选定铍铜作为金属，用这些纤维制作复合材料。因为，这种金属熔点相对较低，940℃，物理常数好。因此很容易在实验室进行治疗。其他测试使用的金属，铜，铅，锡，锌，铝，在处理和加工中是不够的。使用Be-Cu合金的问题是，这种金属与纤维材料的亲密性不好，无法制作复合材料制成的金属丝。这在实验中测试的所有金属中都得到了证明。这些金属丝经电解蚀刻后制成0.5mm厚的针状尖端仪器。这些尖端仪器只能在低到中等放大倍率下进行显微解剖。因为，针的锐度和直径是由单根纤维的直径决定的。但这些尖端仪器具有良好的硬度和刚度，可用于显微解剖。

The grant for the studies on "the improvement of efficiency on the microdissection in the SEM using compound materials : Its development and practice" started just after the delivery of grant for 1991.At first, the tip-instruments for microdissection were made by using tungsten-carbide-needle improved by us. They were sharpened by electrolysis and used for microdisection. At the same time the trial manufacture of new tip-instruments using metall-fibre-composit material started. The fibre materials, carbon fibre and silicon carbide fibre, were supplied from The Research Institute for Iron, Steel, and Other Materials, Tohoku University, ToRey Corporation, Nippon Carbon Co. The Berilium-Copper was finally selected as the metal to make composit material with these fibres. Because, this metal has relatively low melting point, 940C, and good physical constants. So it is very easy to treat in laboratory. Other metals tested for use, Cu, Pb, Sn, Zn, Al, were not sufficient in treatment and processign. The problem to use Be-Cu alloy was that this metal had not good intimacy to fibrous material to make wire made of composit material. This was shown in all the case of metals tested in the experiments.These wire were etched by electrolysis and made to needle shaped tip instruments with 0.5mm thickness. These tip instruments could use only microdissection in low to medium magnification. Because, The sharpness and diameter of the needle was decided by the diametre of the single fiber. But these tip instruments had good hardness and stiffness to use for microdissection.