Study on the inheritance of very dwarfness in Citrus and Poncirus and its use for breeding

柑橘、枳属极矮化遗传及其育种研究

• 批准号: 02660036
• 负责人: WAKANA Akira
• 金额: $ 1.41万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660036/
• 关键词: Poncirus; Cirus; Very dwarfness; Gibberellin; single reseccive dwarf gene; Rootstock; Precocious flowering; Modified gene; 矮性; 単劣性遺伝子; 細胞質遺伝; 不完全優性

Genetical, morphological and histological studies were carried out to demonstrate the characteristics of various types of dwarf seedlings occurred in Citrus and Poncirus. When the polyembryonic cultivars were used as a pistilate parent, zygotic seedlings were determined by isozyme analysis. In the seedlings from self-pollination of 'Flying Dragon', normal and Flying Dragon-shaped seedlings and very dwarf seedlings segregated at the ratio 3:1, indicationg that 'Flying Dragon' is heterozygous for single recessive dwarf gene and that very dwarf seedlings are homozygous for it. This assumption were supported by the evidence that in crosses between citrus cultivars and 'Flying Dragon', normal seedlings were produced.Since the growth of the very dwarf seedlings was promoted by 5-25ppm gibberellin treatment and grafting on normal trifoliate orange, this reseccive dwarf gene is considered to inhibit gibberellin synthesis. The morphological and histological charactors of very dwarf seedlings we … More re very different from those of normal and Flying- Dragon-shaped seedlings.The elongated dwarf seedlings on normal trifoliate orange could be divided into normal and Flying Dragon-shaped types, indicating that Flying Dragon dwarfness was controlled by Flying Dragon dwarf gene. However, we could not clearly resolved the mechanisum of the ingeitance.Since selfed 'Chimotto' produced normal and dwarf seedlings at the ratio 1:3 and since citrus and Poncirus cultivars pollinated with 'Chimotto' produced them at the ratio 1:1 and 15:1, 'Chimotto' type dwarfness is considered to be controlled by dominant major gene which interacts with modified genes. 'Bouquet' type dwarfness is thought to be controlled by single dominant gene which interacts with lethal gene, since seedlings obtained from citrus cultivars pollinated with 'Bouquet' could be divided into dwarf and normal types at the ratio 2:1. Further study for these hypothesis is now in progress.Lastly, the present results suggest that use of 'Flying Dragon' as a pistilate parent is sutable for breeding dwarf rootstocks for citrus, since 10% of zygotic seedlings from self-pollination of 'Flying Dragon' showed precocious flowering. Less

通过对柑桔和枳属植物矮化苗的遗传学、形态学和组织学研究，阐明了柑桔和枳属植物矮化苗的各种类型的特征。以多胚品种作雌蕊亲本，用同工酶分析鉴定合子苗。在‘飞龙’自花授粉的实生苗中，正常苗、飞龙形苗和极矮苗按3：1的比例分离，表明‘飞龙’是一个隐性矮秆基因的杂合，而极矮苗则是纯合的。这一假设得到了柑桔品种与‘飞龙’杂交产生正常苗的证据的支持。由于极矮苗的生长受到5- 25 ppm赤霉素处理和嫁接在正常的枳橙子上的促进，因此认为这一隐性矮秆基因抑制赤霉素的合成。对极矮化苗的形态和组织学特征进行了研究， ...更多信息 与正常幼苗和飞龙形幼苗有很大不同，正常三叶橙子上的细长矮生幼苗可分为正常类型和飞龙形类型，表明飞龙矮生是由飞龙矮生基因控制的。由于‘Chimotot’自交产生正常苗和矮化苗的比例为1：3，用‘Chimotot’授粉的柑橘和枳品种产生正常苗和矮化苗的比例为1：1和15：1，因此认为‘Chimotot’型矮化是由显性主基因与修饰基因互作控制的。“Bouquet”型矮化被认为是由一对显性基因控制的，该基因与致死基因互作，因为从“Bouquet”授粉的柑橘品种获得的幼苗可以按2：1的比例分为矮化型和正常型。最后，本研究结果表明，"飞龙"自花授粉的合子苗中有10%表现早熟开花，因此，利用"飞龙"作母本进行柑橘矮化砧木育种是可行的。少

项目成果

若菜 章,一色 司郎,白石 眞一,花田 信章 安河内 幸一: "ヒリュウに出現する極矮性実生の起源と特性" 園芸学会雑誌. 59(別2). 12-13 (1990)

Akira Wakana、Shiro Isshiki、Shinichi Shiraishi、Nobuaki Hanada、Koichi Yasukochi：“Hiryu 中出现的极矮化幼苗的起源和特征”，日本园艺学会杂志 59（第 2 部分）（1990 年）。

Akira wakana,N.Hanada,S.Isshiki,K.Ureshino,and S.Shiraishi: "Precocious flowering in zygotic seedlings from self-pollination of Poncirus trifoliata cv.Flying Dragon" Hort Science.

Akira wakana、N.Hanada、S.Isshiki、K.Ureshino 和 S.Shiraishi：“Poncirus trifoliata cv.Flying Dragon 自花授粉导致合子幼苗早熟开花”园艺科学。

Akira Wakana,S.Issiki,and S.Shiraishi: "Origin and characteristics of very dwarf seedlings from self-pollinated ‘Flying Dragon'(Poncirus trifoliata)" Scientia Horticulturae.

Akira Wakana、S. Issiki 和 S. Shiraishi：“自花授粉‘飞龙’（枳）极矮化幼苗的起源和特征”Scientia Horticulturae。

若菜 章: "ヒリュウに出現する極矮性実生の起源と特性" 園芸学会雑誌. 59巻別冊2. 12-13 (1990)

Akira Wakana：“飞龙中出现的极矮幼苗的起源和特征”日本园艺学会杂志第 59 卷，特刊 2. 12-13 (1990)。