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Study on the inheritance of very dwarfness in Citrus and Poncirus and its use for breeding

柑橘、枳属极矮化遗传及其育种研究

基本信息

批准号：
02660036
负责人：
WAKANA Akira
金额：
$ 1.41万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660036/
关键词：
Poncirus Cirus Very dwarfness Gibberellin single reseccive dwarf gene Rootstock Precocious flowering Modified gene 矮性単劣性遺伝子細胞質遺伝不完全優性

项目摘要

Genetical, morphological and histological studies were carried out to demonstrate the characteristics of various types of dwarf seedlings occurred in Citrus and Poncirus. When the polyembryonic cultivars were used as a pistilate parent, zygotic seedlings were determined by isozyme analysis. In the seedlings from self-pollination of 'Flying Dragon', normal and Flying Dragon-shaped seedlings and very dwarf seedlings segregated at the ratio 3:1, indicationg that 'Flying Dragon' is heterozygous for single recessive dwarf gene and that very dwarf seedlings are homozygous for it. This assumption were supported by the evidence that in crosses between citrus cultivars and 'Flying Dragon', normal seedlings were produced.Since the growth of the very dwarf seedlings was promoted by 5-25ppm gibberellin treatment and grafting on normal trifoliate orange, this reseccive dwarf gene is considered to inhibit gibberellin synthesis. The morphological and histological charactors of very dwarf seedlings we … More re very different from those of normal and Flying- Dragon-shaped seedlings.The elongated dwarf seedlings on normal trifoliate orange could be divided into normal and Flying Dragon-shaped types, indicating that Flying Dragon dwarfness was controlled by Flying Dragon dwarf gene. However, we could not clearly resolved the mechanisum of the ingeitance.Since selfed 'Chimotto' produced normal and dwarf seedlings at the ratio 1:3 and since citrus and Poncirus cultivars pollinated with 'Chimotto' produced them at the ratio 1:1 and 15:1, 'Chimotto' type dwarfness is considered to be controlled by dominant major gene which interacts with modified genes. 'Bouquet' type dwarfness is thought to be controlled by single dominant gene which interacts with lethal gene, since seedlings obtained from citrus cultivars pollinated with 'Bouquet' could be divided into dwarf and normal types at the ratio 2:1. Further study for these hypothesis is now in progress.Lastly, the present results suggest that use of 'Flying Dragon' as a pistilate parent is sutable for breeding dwarf rootstocks for citrus, since 10% of zygotic seedlings from self-pollination of 'Flying Dragon' showed precocious flowering. Less

通过遗传学、形态学和组织学研究，论证了柑橘和枳属各类矮化苗的特征。当多胚品种用作雌蕊亲本时，通过同工酶分析确定合子幼苗。在‘飞龙’自花授粉苗中，正常苗、飞龙型苗和极矮化苗的分离比例为3:1，表明‘飞龙’为单隐性矮化基因杂合体，极矮化苗为纯合体。这一假设得到了证据的支持，即在柑橘品种与“飞龙”之间的杂交中，产生了正常的幼苗。由于5-25ppm赤霉素处理和嫁接在正常枳橙上促进了非常矮化的幼苗的生长，因此认为该连生矮化基因抑制了赤霉素的合成。极矮化苗的形态和组织学特征与正常苗和飞龙型苗有很大不同。正常枳上的矮化苗可分为正常型和飞龙型，说明飞龙矮化是由飞龙矮化基因控制的。然而，我们无法清楚地解析其发生机制。由于“Chimotto”自交以1：3的比例产生正常和矮化幼苗，并且由于用“Chimotto”授粉的柑橘和枳属品种以1：1和15：1的比例产生正常和矮化幼苗，因此认为“Chimotto”型矮化是由与修饰基因相互作用的显性主基因控制的。 “花束”型矮化被认为是由与致死基因相互作用的单个显性基因控制的，因为用“花束”授粉的柑橘品种获得的幼苗可以按2:1的比例分为矮化型和正常型。对这些假设的进一步研究正在进行中。最后，目前的结果表明，使用“飞龙”作为雌蕊亲本适合培育柑橘矮化砧木，因为“飞龙”自花授粉的合子苗有10%表现出早熟。较少的