Study on the identification of genes related to self-incompatibility and the alleric diversity in Citrus

柑橘自交不亲和及过敏多样性相关基因鉴定研究

• 批准号: 07456021
• 负责人: WAKANA Akira
• 金额: $ 5.06万
• 依托单位: KYUSYU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456021/
• 关键词: Self-incompatibility; Self-incompatibility gene; Semi-self-comoatibility; Semi-cross-compatibility; GOT isozymes; Linkage; Pollen tube; Recombination value; 自家和合性; 半自家和合性; 遺伝子; 対立遺伝子; 制限受粉; 自家受粉; アイソザイム; ひずみ分離; カンキツ; 自家不和合遺伝子; アイソザイム遺伝子

Many Citrus cultivars and accessions were used for various crosses to obtain the progenies GOT isozyme analysis was carried out using the leaf of these progenies. Linkage analyses for Got-1/Got-2, Got-1/Got-3 and Got-2/Got-3 indicated that the three gene pairs did not appear to be closely linked. Out of 32 crosses, distorted single-gene segregation ratios in the progenies were found in four crosses for Got-1, three for Got-2 and ten for Got-3. Amarked deficiency of certain GOT-1 and GOT-3 genotypes in the crosses between unrelated cultivars was attributed to the relatively close linkage (r=0.30*0.05) of Got-1 with the incmpatibility Z locus and the same intensity of linkage (r=0.30*0.05) of Got-3 with the incompatibility S locus. Although it was not obvious whether or not the Z and S loci were the same incompatibility gene loci, the two genes were considered to be different genes by some evidences. Incompatibility genotypes were proposed for several cultivars together with linked Got-1 or Got-3 alleles. A total of 4 allels for Z gene and 7 allels for S gene were found in pumelo and its related hybrid cultivars.

利用柑桔品种和材料进行杂交获得后代，并对后代叶片进行GOT同工酶分析。Got-1/Got-2、Got-1/Got-3和Got-2/Got-3的连锁分析表明，这三个基因对似乎并不紧密连锁。在32个杂交组合中，Got-1有4个杂交组合，Got-2有3个杂交组合，Got-3有10个杂交组合的后代中存在单基因分离比例畸变。Got-1和Got-3基因型在不亲和品种间杂交中的显著缺失是由于Got-1与不亲和Z位点的连锁较近（r=0.30*0.05），Got-3与不亲和S位点的连锁强度相同（r=0.30*0.05）。虽然Z和S基因座是否为同一不亲和基因座尚不明确，但有证据表明这两个基因是不同的基因。与Got-1或Got-3等位基因连锁的几个品种提出了不亲和基因型。在柚类及其近缘杂交种中，Z基因有4个等位基因，S基因有7个等位基因。

项目成果

A.Wakana, X.B.Ngo, and S.Isshiki: "Self-incompatibility in Citrus : Linkage between GOT isozymeloci and the incompatrbility loci." Breeding and Bictechnology for Fruit Trees. 90-93 (1998)

A.Wakana、X.B.Ngo 和 S.Isshiki：“柑橘的自交不亲和性：GOT 同工酶位点与不相容基因座之间的联系。”

Wakana, A., X.B.Ngo and S.Isshiki: "Self-incompatibility in Citrus : Linkage between GOT isozyme loci and the incompatibility loci." Omura, M., T.Hayashi and S.Scott (eds.), National Institute of Fruit Tree Science, Breeding and Biotechnology for Fruit Tr

Wakana, A.、X.B.Ngo 和 S.Isshiki：“柑橘的自交不亲和性：GOT 同工酶位点与不亲和性位点之间的联系。”

A.Wakana,X.B.Ngo,and S,Isshiki: "Self-incompatibility in Citrus;linkage between GOT isogyme lai and the incompatibility leci" Technifues on gene diugnosis and freeding in Fruit trees. in press (1998)

A.Wakana，X.B.Ngo，和S，Isshiki：“柑橘的自交不亲和性；GOT isogyme lai 与不亲和性leci之间的联系”果树基因诊断和释放技术。

ノースンビン,若菜章,若尾英輔: "アイソザイム,制限受粉,及び自家受粉によるカンキツの自家不和合性の検討" 園芸学会雑誌. 66・別冊1. 14-15 (1997)

Northunbin、Akira Wakana 和 Eisuke Wakao：“通过同工酶、限制授粉和自花授粉检查柑橘的自交不亲和性”，日本园艺学会杂志 66，特刊 1. 14-15 (1997)。