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Studies on the cytoplasmic-genic male sterility in acid citrus

酸性柑橘细胞质雄性不育研究

基本信息

批准号：
15380026
负责人：
WAKANA Akira
金额：
$ 7.17万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380026/
关键词：
Citrus Cytoplasmic-genic male sterility Complementary gene Epistasis of gene Cytoplasmic factor for Restoration of male sterility RAPD marker Precocious flowering Acid citrus 優性上位遺伝子香酸カンキツ無核 PAPD 連鎖雌性不稔雄性不稔タペート細胞核-細胞質

项目摘要

This study was focused on the certification of genetic control of male sterility in ten-year-old acid citrus seedlings derived from various cross combinations and the early selection of male sterile hybrid seedlings with RAPD markers. The results of study carried out for three years were as follows.It was found that (1) the male sterility was controlled by independent three genes ms1, ms2 and ms3 that are recessive, (2)additive gene effect was recognized among three genes : ms1 and ms2 are complementary genes and ms3 is a dominant gene with epistasis effect for ms1 and ms2. (3)Amazingly, genotypes of citrus cultivars used in the present study were heterozygous for the ms genes. (4)Cytoplasm of Sudachi had a factor to restore the male sterility in the progenies.Precocious flowering in 3- to 5-month-old hybrid seedlings from crosses with Yuzu, Hanayu, Kabosu and Sudachi was observed. Monoembryonic and male sterile HY-16, which was derived from Hanayu x Yuzu, was produced precocious hybrid seedlings with high frequencies (6% for HY16 x Yuzu and 16% for HY16 x Foster Pink grapefruit). In these seedlings, male sterile and fertile seedlings segregated 5 : 3 for HY16 x Yuzu and 1: 1 for HY16 x Foster Pink grapefruit, suggesting the estimated male sterility genotypes for HY16 and Yuzu were correct.Based on these results, genotypes of 12 citrus cultivars were determined. Furthermore, RAPD markers linked to ms genes were studies with a bulk method and analyzed with about 100 random primers. One RAPD marker as to CMN-A10 primer was found to link to ms1 gene with a distance of 6.3 cM. The cytological observation of anther development in male sterile seedlings indicated that microsporogenesis were inhibited before and after meiosis.

本研究以不同杂交组合的10年生酸橙实生苗为材料，对其雄性不育性的遗传控制进行了验证，并对雄性不育杂交种的早期选择进行了研究。经过3年的研究，结果表明：(1)雄性不育由MS1、MS2、MS3三个独立的隐性基因控制；(2)MS1、MS2为互补基因，MS3为显性上位效应基因。(3)令人惊讶的是，本研究所用柑橘品种的ms基因是杂合的。(4)苏达的细胞质是恢复后代雄性不育的一个因素，3~5月龄的杂交苗木与玉树、花玉、卡博苏、苏达杂交，均能观察到早花现象。以花玉×油柚为父本选育的单胚性雄性不育系HY-16，获得了早熟、早熟的高频率杂种苗(HY16×油柚6%，HY16×福斯特粉柚16%)。结果表明，HY16×油柚和HY16×福斯特粉柚的雄性不育和可育苗比例分别为5：3和1：1，表明HY16和油柚的雄性不育类型是正确的。此外，还对与ms基因连锁的RAPD标记进行了批量研究，并用大约100个随机引物进行了分析。CMN-A10标记中有1个与MS1基因连锁，距离为6.3 cM。雄性不育苗花药发育的细胞学观察表明，减数分裂前后小孢子发生受到抑制。