Studies on the cytoplasmic-genic male sterility in acid citrus

酸性柑橘细胞质雄性不育研究

基本信息

批准号：
15380026
负责人：
WAKANA Akira
金额：
$ 7.17万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380026/
关键词：
Citrus Cytoplasmic-genic male sterility Complementary gene Epistasis of gene Cytoplasmic factor for Restoration of male sterility RAPD marker Precocious flowering Acid citrus 優性上位遺伝子香酸カンキツ無核 PAPD 連鎖雌性不稔雄性不稔タペート細胞核-細胞質

项目摘要

This study was focused on the certification of genetic control of male sterility in ten-year-old acid citrus seedlings derived from various cross combinations and the early selection of male sterile hybrid seedlings with RAPD markers. The results of study carried out for three years were as follows.It was found that (1) the male sterility was controlled by independent three genes ms1, ms2 and ms3 that are recessive, (2)additive gene effect was recognized among three genes : ms1 and ms2 are complementary genes and ms3 is a dominant gene with epistasis effect for ms1 and ms2. (3)Amazingly, genotypes of citrus cultivars used in the present study were heterozygous for the ms genes. (4)Cytoplasm of Sudachi had a factor to restore the male sterility in the progenies.Precocious flowering in 3- to 5-month-old hybrid seedlings from crosses with Yuzu, Hanayu, Kabosu and Sudachi was observed. Monoembryonic and male sterile HY-16, which was derived from Hanayu x Yuzu, was produced precocious hybrid seedlings with high frequencies (6% for HY16 x Yuzu and 16% for HY16 x Foster Pink grapefruit). In these seedlings, male sterile and fertile seedlings segregated 5 : 3 for HY16 x Yuzu and 1: 1 for HY16 x Foster Pink grapefruit, suggesting the estimated male sterility genotypes for HY16 and Yuzu were correct.Based on these results, genotypes of 12 citrus cultivars were determined. Furthermore, RAPD markers linked to ms genes were studies with a bulk method and analyzed with about 100 random primers. One RAPD marker as to CMN-A10 primer was found to link to ms1 gene with a distance of 6.3 cM. The cytological observation of anther development in male sterile seedlings indicated that microsporogenesis were inhibited before and after meiosis.

这项研究的重点是从各种交叉组合得出的十岁酸柑橘幼苗中男性不育的遗传控制证明，并早期选择了用RAPD标记的雄性无菌杂种幼苗。进行了三年的研究结果如下。（1）雄性不育由具有隐性的独立三个基因MS1，MS2和MS3控制，（2）在三个基因中识别添加基因效应：MS1和MS2在互补的基因中，MS3是互补的，MS3是具有Epistasis效应MS1和MS2的主要基因。（3）令人惊讶的是，本研究中使用的柑橘类品种的基因型是MS基因的杂合子。（4）Sudachi的细胞质具有恢复后代中男性无菌性的因素。从Yuzu，Hanayu，Kabosu和Sudachi的十字架上进行了3至5个月大的杂交幼苗的菜谱。源自Hanayu X Yuzu的单共晶和雄性无菌HY-16是具有高频的早熟杂交幼苗（HY16 X Yuzu的6％，HY16 X X Foster Pink Pink Grapefruit）产生。在这些幼苗中，Hy16 X Yuzu的雄性无菌和肥沃的幼苗隔离为5：3，Hy16 X foster Pink粉红色葡萄柚为1：1，这表明HY16和Yuzu的估计男性无菌基因型是正确的。基于这些结果，确定了12种柠檬酸基因型的基因型。此外，与MS基因相关的RAPD标记是用大量方法进行的研究，并用大约100个随机引物进行了分析。发现与CMN-A10引物有关的一个RAPD标记与距离为6.3 cm的MS1基因相连。雄性无菌幼苗的花药发育的细胞学观察表明，微量生成受到减数分裂前后的抑制。