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Studies on the cytoplasmic-genic male sterility in acid citrus

酸性柑橘细胞质雄性不育研究

基本信息

批准号：
15380026
负责人：
WAKANA Akira
金额：
$ 7.17万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380026/
关键词：
Citrus Cytoplasmic-genic male sterility Complementary gene Epistasis of gene Cytoplasmic factor for Restoration of male sterility RAPD marker Precocious flowering Acid citrus 優性上位遺伝子香酸カンキツ無核 PAPD 連鎖雌性不稔雄性不稔タペート細胞核-細胞質

项目摘要

This study was focused on the certification of genetic control of male sterility in ten-year-old acid citrus seedlings derived from various cross combinations and the early selection of male sterile hybrid seedlings with RAPD markers. The results of study carried out for three years were as follows.It was found that (1) the male sterility was controlled by independent three genes ms1, ms2 and ms3 that are recessive, (2)additive gene effect was recognized among three genes : ms1 and ms2 are complementary genes and ms3 is a dominant gene with epistasis effect for ms1 and ms2. (3)Amazingly, genotypes of citrus cultivars used in the present study were heterozygous for the ms genes. (4)Cytoplasm of Sudachi had a factor to restore the male sterility in the progenies.Precocious flowering in 3- to 5-month-old hybrid seedlings from crosses with Yuzu, Hanayu, Kabosu and Sudachi was observed. Monoembryonic and male sterile HY-16, which was derived from Hanayu x Yuzu, was produced precocious hybrid seedlings with high frequencies (6% for HY16 x Yuzu and 16% for HY16 x Foster Pink grapefruit). In these seedlings, male sterile and fertile seedlings segregated 5 : 3 for HY16 x Yuzu and 1: 1 for HY16 x Foster Pink grapefruit, suggesting the estimated male sterility genotypes for HY16 and Yuzu were correct.Based on these results, genotypes of 12 citrus cultivars were determined. Furthermore, RAPD markers linked to ms genes were studies with a bulk method and analyzed with about 100 random primers. One RAPD marker as to CMN-A10 primer was found to link to ms1 gene with a distance of 6.3 cM. The cytological observation of anther development in male sterile seedlings indicated that microsporogenesis were inhibited before and after meiosis.

本研究利用RAPD分子标记技术，对不同杂交组合的10年生酸橙实生苗进行雄性不育性遗传控制的验证和雄性不育杂种实生苗的早期选择。经过三年的研究，结果表明：（1）雄性不育性由独立的三对隐性基因ms 1、ms 2和ms 3控制;（2）三对基因之间存在加性基因效应：ms 1和ms 2为互补基因，ms 3为显性基因，对ms 1和ms 2具有上位性效应。（3）本研究所用柑橘品种的ms基因均为杂合型。（4）须立的胞质具有恢复后代雄性不育的作用，与柚子、花鱼、卡波须、须立杂交的3 ~ 5月龄杂种苗均出现早花现象。以花柚×柚子的单胚雄性不育系HY-16为材料，经杂种优势筛选，获得早熟实生苗率较高（HY-16 ×柚子为6%、HY-16 × Foster Pink柚为16%）。结果表明，HY 16 ×柚子的不育苗与可育苗分离比例为5：3，HY 16 × Foster Pink葡萄柚的不育苗与可育苗分离比例为1：1，表明HY 16和柚子的不育基因型估计是正确的。此外，用批量法对与ms基因连锁的RAPD标记进行了研究，用约100个随机引物进行了分析。利用CMN-A10引物筛选出一个与ms 1基因连锁的RAPD标记，与ms 1基因的连锁距离为6.3cM。对不育苗花药发育的细胞学观察表明，减数分裂前后小孢子发生受到抑制。