Research on isolation and culture of forest tree species

森林树种分离培养研究

• 批准号: 02660154
• 负责人: IDE Yuji
• 金额: $ 1.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660154/
• 关键词: Protoplasts; Betula platyphylla; Quercus acutissima; Acacia mangium; Betula grossa; isolation; Colony formation; Callus formation; アカシア・マンギウム; ケヤキ; オノオレカンバ; コロニー; カルス再生; コロニ-; チョウセンヤマナラシ; マイクロカルス; サスペンションカルチャ-

In this research project, isolation and culture of protoplasts from forest tree species were investigated.Tree species belonging to Fagaceae and Betulaceae which are major broad leaved tree species in Japanese forests and Acacia mangium which is most promising tree species for tropical afforestration were used for research materials.As the results, protoplasts were successfully isolated from Quercus acutissima, Betula platyphylla var japonica, Betula grossa, Betula schmidtii, Zelkova serrata and Acacia mangium.And successful callus regeneration was induced by the culture of protoplasts isolated from somatic embryos of Quercus acutissima.By this research, basic components of enzymes solution and protocols for protoplast isolation from woody plants.

本研究以日本主要阔叶树种壳斗科和桦木科的树种和热带造林最有希望的树种马占相思为材料，对日本主要阔叶树种麻栎、白桦、大桦、大桦、大本研究通过对麻栎体细胞胚原生质体的培养，成功地诱导出愈伤组织，并对酶液的基本组成和木本植物原生质体分离的方法进行了研究。

项目成果

Yuji IDE: "Isolation and culture of mesophyll protoplasts from in vitro subcultured Japanese white birch" Bull.Tokyo Univ. For.84. 53-58 (1991)

Yuji IDE：“体外传代培养的日本白桦叶肉原生质体的分离和培养”Bull.Tokyo Univ.

Yuji Ide,: "Isolation and culture of protoplasts from Quercus acutissima shoot cultures." Bull. Tokyo Univ. For.85. 43-48 (1991)

Yuji Ide，：“麻栎芽培养物中原生质体的分离和培养。”

原口 雅人: "クヌギ不定胚からのプロトプラストの単離" 第102回日本林学会大会論文集. (1991)

Masato Haraguchi：“从锯齿体细胞胚胎中分离原生质体”日本林业学会第 102 届年会论文集（1991 年）。

原口 雅人: "クヌギ不定胚からのプロトプラストの単離" 102回日林論. 383-384 (1991)

原口正人：“从锯齿体细胞胚胎中分离原生质体”，第 102 期 Nihon Hayashiron，383-384（1991 年）。

Yuji IDE: "Isolation and culture of protoplasts from Quercus acutissima shoot cultures" Bull.Tokyo Univ. For.85. 43-48 (1991)

Yuji IDE：“麻栎芽培养物中原生质体的分离和培养”Bull.Tokyo Univ.