Proliferation of double negative (DN) gammadelta T cells in reseponse to allogeneic MHC antigen.

双阴性 (DN) gammadelta T 细胞响应同种异体 MHC 抗原而增殖。

• 批准号: 02670206
• 负责人: NAKAYAMA Eiichi
• 金额: $ 1.47万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670206/
• 关键词: T-cells; Double negative; gammadelta; T細胞抗原レセプタ-(TCR); γδT細胞; T細胞機能; TL抗原; トランスジェニックマウス; T細胞抗原レセプタ-(αδ); ダブル・ネガティブ細胞

1. In TL transgenic mouse strain C3H.Tg.Tla^a-3.1, gammadelta T cells start to increase from three weeks after birth and reach to about 30% of total T cells in peripheral lymphoid tisene. It was demonstrated that gammadelta T cells in these mice respond to proliferate in stimulation with immobilized anti-TCR delta mAb.2. Spleen cells from C3H.Tg.Tla^a-3.1 mice proliferated in response to mitomycin C (MMC) treated BALB/c stimulator cells. In these cultures, proliferative response was observed with gammadelta as well as alphabeta T cells. However, when alphabeta T cells were eliminated before MLC, no prolifenation was observed with gammadelta T cells. Thus, proliferative response of gammadelta T cells was dependent on alphabeta T cells. gammadelta T cells recovered after MLR culture showed no cytotoxicity against ^<51>Cr-labeled BALB/c target cells.

1.在TL转基因小鼠品系C3H.Tg.Tla^a-3.1中，Gammadelta T细胞在出生后3周开始增加，约占外周淋巴T细胞总数的30%。研究表明，这些小鼠体内的Gammadelta T细胞在固定化的抗TCR Delta单抗的刺激下产生增殖反应。丝裂霉素C(MMC)刺激BALB/c小鼠后，C3H.Tg.Tla^a-3.1小鼠脾细胞增殖。在这些培养物中，观察到Gammadelta和Alphabeta T细胞的增殖反应。然而，当在MLC之前消除Alphabeta T细胞时，并没有观察到Gammadelta T细胞的增殖。因此，Gammadelta T细胞的增殖反应依赖于Alphabeta T细胞。经MLR培养后恢复的Gammadelta T细胞对标记的BALB/c靶细胞无细胞毒作用。

项目成果

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