Proliferation of double negative (DN) gammadelta T cells in reseponse to allogeneic MHC antigen.

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1. In TL transgenic mouse strain C3H.Tg.Tla^a-3.1, gammadelta T cells start to increase from three weeks after birth and reach to about 30% of total T cells in peripheral lymphoid tisene. It was demonstrated that gammadelta T cells in these mice respond to proliferate in stimulation with immobilized anti-TCR delta mAb.2. Spleen cells from C3H.Tg.Tla^a-3.1 mice proliferated in response to mitomycin C (MMC) treated BALB/c stimulator cells. In these cultures, proliferative response was observed with gammadelta as well as alphabeta T cells. However, when alphabeta T cells were eliminated before MLC, no prolifenation was observed with gammadelta T cells. Thus, proliferative response of gammadelta T cells was dependent on alphabeta T cells. gammadelta T cells recovered after MLR culture showed no cytotoxicity against ^<51>Cr-labeled BALB/c target cells.