Profiling Analysis of Sugar Phosphates, Sugar Alcohols and Sugar Acids and Its Application to The Studies in Disorders of Sugar Metabolism.

糖磷酸盐、糖醇和糖酸的谱分析及其在糖代谢紊乱研究中的应用。

• 批准号: 02670430
• 负责人: SHIGEMATSU Yosuke
• 金额: $ 0.51万
• 依托单位: Fukui Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670430/
• 关键词: sugar phosphate; sugar alcohol; sugar acid; fructose-1, 6-diphosphatase; diabetes mellitus; organic acid; liquid chromatography; tandem mass spectrometry; 糖代謝異常

In order to perform the pathophysiologic analysis in disorders of sugar and organic acid metabolism, the methods to profile the abnormal metabolites in body fluids of the patients with these disorders were investigated. Quantitative and profiling analyses of sugar, sugar alcohol and sugar acid were done using capillary gas chromatography and mass spectrometry. Using this technique, a series of unique aldonic acids were found in the urine samples of healthy newboms and diabetic human and canine. The analysis of plasma and urine using this method revealed that these aldonic acids were produced by canine after aldose loading. Capillary gas chromatographic analysis of trimethylsilyl acid fraction extracted from the urine using an ion exchange method was found to be a practical and useful method to diagnose fructose- 1, 6diphosphatase deficiency. In addition, diagnostically important sugar phosphates, fructose- 1, 6diphosphate and 3-phosphoglycerate, and glycemldehyde were found to be excreted in the urines of the patients with this disorder. Since some ketose phosphates, especially fructose-1, 6diphosphate, were degenerated to some extent during derivatization, however, the measurements of underivatized sample were studied. Although FAB-MS/MS analysis was not a sensitive method to measure these sugar phosphates, LC/MS/MS with ionspray interface was found to produce molecular ions of sugar phosphates with high intensity, which seems to enable MS/MS analysis. It is thought that the latter technique may be a sensitive and useful method to profile sugar phosphate in biological samples.

为了进行糖和有机酸代谢紊乱的病理生理分析，研究了这些疾病患者体液中异常代谢物的分析方法。采用毛细管气相色谱-质谱联用技术对糖、糖醇和糖酸进行了定量和谱分析。利用该技术，在健康新生儿、糖尿病人和犬的尿样中发现了一系列独特的醛糖酸。使用该方法分析血浆和尿液显示，这些醛糖酸由狗在醛糖负荷后产生。使用离子交换法从尿液中提取三甲基硅酸部分的毛细管气相色谱分析被发现是诊断果糖-1，6二磷酸酶缺乏症的实用且有用的方法。此外，诊断上重要的糖磷酸盐，果糖-1，6二磷酸和3-磷酸甘油酸，和甘氨醛被发现排泄在患者的尿液中。但由于某些磷酸酮糖，特别是1，6-二磷酸果糖在衍生化过程中发生了一定程度的降解，因此对未衍生化样品的测定进行了研究。虽然FAB-MS/MS分析不是测量这些糖磷酸盐的灵敏方法，但发现具有离子喷雾界面的LC/MS/MS产生具有高强度的糖磷酸盐分子离子，这似乎能够进行MS/MS分析。据认为，后者的技术可能是一个敏感的和有用的方法来分析糖磷酸在生物样品。