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The Role of Fimbriae of Escherichia coli in Genitourinary Tract Infection.

大肠杆菌菌毛在泌尿生殖道感染中的作用。

基本信息

批准号：
02670708
负责人：
KUMON Hiromi
金额：
$ 0.83万
依托单位：
Okayama Unversity
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670708/
关键词：
Genitourinary Infection Escherichia coli Fimbriae 尿路感染症病原性因子

项目摘要

Bacterial properties of Escherichia coli isolated from patients with acute uncomplicated infections were examined mainly to clarify the significance of fimbriae as the virulence factor of E. coli in genitourinary tract infections. Then, a simultaneous presence of MS and MR fimbriae including P is considered to be one of the most important virulence factors in genitourinary infections.The author developed a new experimental system using an alloxan induced diabetic mouse and an extremely small volume of inoculum in order to analyze the first event of the establishment of infection. Three variants were developed from OEC-795, which possessed flagella (F) and MS fimbriae. Using the parent (MS+・F+) and three variants (MS+・F-, MS-・F+, MS-・F-), the role of MS fimbriae was re-examined in this experimental system. Consequently, the significance of MS fimbriae in the establishment of infection was demonstrated clearly.The distribution of receptor for MS-fimbriated E. coli on human urothelial cells was also examined. MS-fimbriated E. coli adhered to the surface of urothelium except for that of the bladder luminal superficial cell. The distribution of high-mannose oligosaccharide of asparagine-linked glycoprotein, as an actual receptor moiety for MS-fimbriae was analyzed mainly with backscatter mode of SEM using colloidal gold conjugated Con A (G-Con A). Extremely rare distribution of G-Con A was observed on the surface of the bladder luminal cell. However, a relatively dense distribution of G-Con A was detected on the surfece of normal ureter and renal pelvis not to mention on that of urothelial cancer.

对急性单纯性感染患者分离的大肠埃希菌进行了细菌学特性的研究，主要是为了阐明菌毛作为大肠埃希菌毒力因子的意义。泌尿生殖道感染中的大肠杆菌。同时存在的MS和MR菌毛，包括P被认为是最重要的毒力因子之一，在泌尿生殖道infecties.The作者开发了一个新的实验系统，使用四氧嘧啶诱导的糖尿病小鼠和一个非常小的体积的接种，以分析建立感染的第一个事件。从OEC-795中分离出3个具有鞭毛和MS菌毛的变异体。利用亲本（MS+·F+）和三个变体（MS+·F-、MS-·F+、MS-·F-），在该实验系统中重新研究了MS菌毛的作用。因此，阐明了MS菌毛在感染建立中的意义。大肠杆菌对人尿路上皮细胞的作用。MS菌毛E.除膀胱腔表面细胞外，其余细胞均粘附于膀胱壁表面。用胶体金标记的ConA（G-ConA）作为扫描电镜的背散射模式，分析了作为MS菌毛受体的天冬酰胺糖蛋白的高甘露糖寡糖的分布。G-ConA在膀胱腔细胞表面的分布极为罕见。而G-ConA在正常输尿管和肾盂表面呈相对密集的分布，在尿路上皮癌表面则无明显分布。