Relationships between diabetes mellitus and oral bacteria in periodontitis.

糖尿病与牙周炎口腔细菌之间的关系。

• 批准号: 08672179
• 负责人: HORIUCHI Hiroshi
• 金额: $ 1.41万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672179/
• 关键词: Diabetes mellitus; Periodonto-pathic Bacteria; HL-60; Experimental periodontitis; Superoxide related enzyme; fibroblast; 糖尿病ラット; ストレプトゾトシン; 一酸化窒素産生酵素; 実験的糖尿病; ストレプトゾトシン誘発; 好中球; 遊走能; インシュリン・コントロール; インスリン; 無菌飼育ラット; ストレプトンゾトシン; 普通飼育ラット

Effects of Blood Glucose Control on Experimental Periodontitis in Streptozotocin-induced Diabetic Rats Effects of Insulin Therapy in Conventional and Germ Free RatsIt was evaluated the effect of the glycemic control on the periodontal condition of streptozotocin-induced diabetic rats with experimental periodontitis. Conventional and germ-free rats were divided into normal rats and insulin-treated rats. An elastic ring was placed around the right mundibular 1St molar. The evaluation of the periodontal condition was made on 14 and 28 days. Blood glucose level, the extent of the inflammatory change, and the quantitative measurement of the alveolar bone area were recorded. The insertion of elastic ring in the periodontal crevice induced attachment loss and alveolar bone resorption. The alveolar bone area of the experimental side in the non-diabetes mellitus group and diabetes mellitus-insulin control group were significantly larger than in the diabetes mellitus group on 14 and 28 days.Induction of Superoxide related enzymes in HL-60 and human gingival fibroblasts stimulated by periodonto-pathic bacteriaThe HL-6O and fibroblasts were incubated with periodonto-pathic bacteria. The HL-60 was evaluated the existence of Superoxide Dismutase and catalase , fibroblasts was estimated by the existence of nitric oxide synthase in each cell. Each enzyme was stained by FITO-laveled indirect-immunological antibody method. The superoxide dismutase and catalase were widely spread in the all cells. The nitric oxide synthase was spread in small area.

血糖控制对链脲佐菌素诱导的糖尿病大鼠实验性牙周炎的影响胰岛素治疗对常规和无菌大鼠的影响本实验观察血糖控制对链脲佐菌素诱导的糖尿病大鼠实验性牙周炎牙周状况的影响。常规组和无菌组大鼠分为正常组和胰岛素治疗组。在右下颌第一磨牙周围放置弹性环。在第14天和第28天对牙周状况进行评估。记录血糖水平、炎症改变程度和牙槽骨面积的定量测量。弹性环置入牙周裂隙导致附着丧失和牙槽骨吸收。非糖尿病组和糖尿病胰岛素对照组实验侧牙槽骨面积在第14天和第28天均显著大于糖尿病组。牙周病细菌对HL-60和人牙龈成纤维细胞超氧化物歧化酶的诱导作用通过检测HL-60细胞中超氧化物歧化酶和过氧化氢酶的存在，通过检测各细胞中一氧化氮合酶的存在来检测成纤维细胞。用FITC标记的间接免疫抗体法对各酶进行染色。超氧化物歧化酶和过氧化氢酶广泛分布于所有细胞中。一氧化氮合酶呈小面积分布。

项目成果

加藤清: "Streptozotocin誘導糖尿病ラットに対する血糖コントロールが実験的歯周炎に及ぼす影響 1.普通飼育ラットにおけるインスリンの効果" 日本歯周病学会会誌. 38巻・4号. 400-414 (1996)

加藤清：“血糖控制对链脲佐菌素诱导的糖尿病大鼠实验性牙周炎的影响1。胰岛素对正常饲养的大鼠的影响”日本牙周病学会杂志，第38卷，第4.400-414期（1996年）。

堀内 博: "ハイリスク感染症患者の歯科ケア" 社団法人 仙台歯科医師会, 4 (1996)

堀内浩：“高危传染病患者的牙科护理”仙台牙科协会，4（1996）