Formation Mechanism of Tea Aroma and Search for Precursor

茶香气的形成机制及前体的寻找

基本信息

  • 批准号:
    02680052
  • 负责人:
  • 金额:
    $ 1.15万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1990
  • 资助国家:
    日本
  • 起止时间:
    1990 至 1991
  • 项目状态:
    已结题

项目摘要

From the hot water extract of tea leaves, glycoside fraction was obtained as butanol extract, which was acetylated to separate each compounds. Through silica gel column, two fractions, BA-6 and BA-7, were shown to be single compound on the thin layer chromatogram.The former was identified with (Z)-3-hexenyl-beta-D-glucopyranoside tetraacete based on the spectrometric analyses. The latter was identical with synthetic benzyl-beta-D-glucopyranoside tetraacetate in all physical and spectrometric aspects.The crude glycoside fraction was also isolated from green tea which was enzymatically hydrolyzed with acetone powder prepared from fresh tea leaves and commercial beta-D-glucosidase. Non-enzymatic hydrolysis with hydrochloric acid was also applied. The volatile compounds formed after different hydrolysis were almost same, however, large amounts of benzyl alcohol and (Z)-3-hexenol were obtained after the hydrolysis with beta-D-glucosidase. This result is consistent with the presence of beta-D-glucosides of these volatiles in fresh tea leaves as aroma precursor.
从茶叶热水浸出物中分离得到糖苷组分丁醇浸出物,再进行乙酰化反应,分离出各组分。通过硅胶柱层析,得到BA-6和BA-7两个组分,经光谱分析鉴定为(Z)-3-己烯基-β-D-吡喃葡萄糖苷四乙酸酯。后者与人工合成的苄基-β-D-葡萄糖苷四乙酸酯在物理和光谱方面都是一致的。绿茶用新鲜茶叶制备的丙酮粉和商品β-D-葡萄糖苷酶酶解绿茶中也得到了粗苷。此外,还采用了非酶盐酸水解法。不同酶解后得到的挥发性化合物基本相同,但经β-D-葡萄糖苷酶酶解后得到大量的苯甲醇和(Z)-3-己烯醇。这一结果与这些挥发物中的β-D-葡萄糖苷作为香气前体在新鲜茶叶中的存在是一致的。

项目成果

期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Motoko YANO: "Benzyl Glucoside from Tea Leaves" Agricultural and Biological Chemistry(Agric.Biol.Chem.). 55. 1205 (1991)
Motoko YANO:“茶叶中的苄基葡萄糖苷”农业和生物化学(Agric.Biol.Chem.)。
  • DOI:
  • 发表时间:
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    0
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  • 通讯作者:
M.Yano: "Benzgl Glucoside from Tea Leaves." Agricultnral and Biological Chenistry(Agric.Biol.Chem.). 55. 1205-1206 (1991)
M.Yano:“茶叶中的 Benzgl 葡萄糖苷。”
  • DOI:
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  • 影响因子:
    0
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  • 通讯作者:
Motoko Yano: "Benzyl Glucoside from Tea Leaves" Agric. Biol. Chem.55. 1205 (1991)
Motoko Yano:“茶叶中的苄基葡萄糖苷”农业。
  • DOI:
  • 发表时间:
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  • 影响因子:
    0
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  • 通讯作者:
M.Yano: "Benzgl Glucoside from Tea Leaves" Agricultural and Biological Chemistry (Agric.Biol.Chem.). 55. 1205-1206 (1991)
M.Yano:“茶叶中的 Benzgl 葡萄糖苷”农业和生物化学(Agric.Biol.Chem.)。
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    0
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KOBAYASHI Akio其他文献

Comparative Evaluation of Diverse Features in Fluency Evaluation of Spontaneous Speech
自发语音流利度评价中不同特征的比较评价
  • DOI:
    10.1587/transinf.2022edp7047
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    0.7
  • 作者:
    DENG Huaijin;UTSURO Takehito;KOBAYASHI Akio;NISHIZAKI Hiromitsu
  • 通讯作者:
    NISHIZAKI Hiromitsu
普通の教師が生きる学校 モンスター・ペアレント論を超えて(第495回)「担任代われ」が普通に出る時代(5)都市部から全国へ
普通教师居住的学校:超越怪物家长理论(第495期)“代课班主任”盛行的时代(五)从城市到全国
  • DOI:
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    0
  • 作者:
    DENG Huaijin;UTSURO Takehito;KOBAYASHI Akio;NISHIZAKI Hiromitsu;小野田正利
  • 通讯作者:
    小野田正利

KOBAYASHI Akio的其他文献

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{{ truncateString('KOBAYASHI Akio', 18)}}的其他基金

Investigation on the mechanism and predicting model of formation of sand layer on the gravel bed
砾石层砂层形成机理及预测模型研究
  • 批准号:
    25420531
  • 财政年份:
    2013
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Studies of aphthous stomatitis on the response to glucocorticoids or immunocompetence of γδT cells.
口疮性口炎对糖皮质激素反应或γδT细胞免疫能力的研究。
  • 批准号:
    20592343
  • 财政年份:
    2008
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The correlation between CYP3A activity and the risk of osteonecrosis.
CYP3A活性与骨坏死风险之间的相关性。
  • 批准号:
    17591590
  • 财政年份:
    2005
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Mutual interaction of glycosides and enzymes in the development of plant food aroma
糖苷和酶在植物性食品香气形成中的相互作用
  • 批准号:
    08456064
  • 财政年份:
    1996
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of new antimicrobial pressure sensitive adhesive polymer for surgical tape
开发用于手术胶带的新型抗菌压敏粘合剂聚合物
  • 批准号:
    08555204
  • 财政年份:
    1996
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Diagnosis of plant disease by analysis of peptides and oligosaccharides in the sap.
通过分析汁液中的肽和寡糖来诊断植物病害。
  • 批准号:
    08456061
  • 财政年份:
    1996
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
A basic research for the molecular of plant defense system and an application for agricultre.
植物防御系统分子基础研究及其农业应用。
  • 批准号:
    06660136
  • 财政年份:
    1994
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Microtubulin assembly inhibitors from microorg origin
来自微生物来源的微管蛋白组装抑制剂
  • 批准号:
    63560130
  • 财政年份:
    1988
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Formation of terpenoids contributing to tea aroma.
萜类化合物的形成有助于茶的香气。
  • 批准号:
    62560121
  • 财政年份:
    1987
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Effects of IgE and complement on the protective immunity against helimth infections.
IgE 和补体对蠕虫感染保护性免疫的影响。
  • 批准号:
    62480150
  • 财政年份:
    1987
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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使用功能修饰的糖苷水解酶对真菌细胞壁多糖进行结构分析
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