A basic research for the molecular of plant defense system and an application for agricultre.

植物防御系统分子基础研究及其农业应用。

• 批准号: 06660136
• 负责人: KOBAYASHI Akio
• 金额: $ 0.38万
• 依托单位: Osaka University (1995)Okayama University (1994)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660136/
• 关键词: Plant; Resistance; Secondary metabolism; Elicitor; Oligosaccharide; Polysaccharide; Laminaran; 糖類

Plants quickly respond to pathogenic invasion. As the result, the secondary metabolism is activated. Therefore, we may use the information obtained from this plant-microbe interaction for secondary metabolite production. For this purpose, we are very much interested in what type of stimulating factors are involved in the activation of the metabolism. We investigated the stimulating factors and we attempted to prepare those, since these eliciting factors can be applicable to produce new secondary metabolites in the plant cell system and a large suply is required. The results of our investigation were listed below.1) The elicitor activities of deacetylated chitin oligomers to legume plants were investigated.2) A facile method for preparation of deacetylated chitin oligomers using lysozyme was developed3) Elicitor-active oligosaccharides from laminaran was purified and thier structures were elucidated.4) A structure-activity relationship study for elicitor-active oligosaccharide from laminaran was performed.

植物对病原体入侵反应迅速。结果，次级代谢被激活。因此，我们可以使用从这种植物-微生物相互作用中获得的信息用于次级代谢产物的生产。为此，我们非常感兴趣的是什么类型的刺激因子参与了新陈代谢的激活。我们研究了刺激因子，并试图制备这些刺激因子，因为这些诱导因子可用于在植物细胞系统中产生新的次生代谢产物，并且需要大量的供应。主要研究结果如下：1）研究了脱乙酰甲壳素低聚物对豆科植物的激发子活性; 2）建立了一种简便的溶菌酶法制备脱乙酰甲壳素低聚物; 3）从海带多糖中分离纯化了具有激发子活性的低聚糖，并对其结构进行了鉴定; 4）对海带多糖中具有激发子活性的低聚糖进行了构效关系研究。

项目成果

A. Kobayashi, et. al.: "A novel method for chemo-enzymatic synthesis of elicitor-active chitosan oligomers and partially N-deacetylated chitin oligowers using N-acylated chitotrises as substrates in a lysozyme-catalyzed transglycosylation veation" Carbohy

A.小林等。

A.Kobayashi, K.Akiyama, K.Kawazu: "Partially N-Deacetylated Chitin Fragments are Strong Elicitors for (+)-Pisatin Induction in Epicotyls of Pea." Z.Naturforsch.49c. 302-308 (1994)

A.Kobayashi、K.Akiyama、K.Kawazu：“部分 N-脱乙酰甲壳素片段是豌豆上胚轴中 ( )-Pisatin 诱导的强诱导子。”

A.Kobayashi: "Antifungal compounds induced in the dual culture with Phytolacca americana callus and Botrysis fabae" Z.Naturforsch.50c. in printing (1995)

A.Kobayashi：“在美洲商陆愈伤组织和蚕豆葡萄孢的双重培养物中诱导的抗真菌化合物”Z.Naturforsch.50c。

K.Akiyama, K.Kawazu, and A.Kobayashi: "A novel method for chemo-enzymatic synthesis of elicitor-active chitosan oligomers andpartially N-deacetylated chitin oligomers using N-acylated chitotrioses as substrates in a lysozyme-catalyzed transglycosylation r

K.Akiyama、K.Kawazu 和 A.Kobayashi：“一种在溶菌酶催化的转糖基化反应中使用 N-酰化壳三糖作为底物化学酶合成引发剂活性壳聚糖低聚物和部分 N-脱乙酰甲壳素低聚物的新方法

A.Tai, E.Osawa, K.Kawazu, and A.Kobayashi: "A Minimun Essential Structure of LN-3 Elicitor Activity in Been Cotyledons" Z.Naturforsch.51c (in press). (1996)

A.Tai、E.Osawa、K.Kawazu 和 A.Kobayashi：“子叶中 LN-3 诱导子活性的最小基本结构”Z.Naturforsch.51c（印刷中）。