Diagnosis of plant disease by analysis of peptides and oligosaccharides in the sap.

众所周知，植物利用从土壤中通过根吸收的无机元素进行代谢。另一方面，植物根际也存在一些小的有机化合物，如土壤微生物的代谢物或人工污染物。这些有机化合物对植物的影响尚不清楚。首先，我们研究了水稻对根际微生物代谢产物的吸收，以阐明有机化合物介导的根际微生物之间的关系。我们阐明了水稻植株喜欢吸收2-氨基苯甲酰胺，它是放线菌的主要代谢产物之一。为了阐明2-氨基苯甲酰胺的吸附动力学，我们对其从水稻植株中的衍生物进行了纯化和鉴定。我们用甲醇提取稻根，得到一种可能是2-氨基苯甲酰胺代谢产物的化合物，命名为OMICRON-ABM-1 OMICRON-ABM-2，OMICRON-ABZN-3。进一步的波谱分析表明，OMICRON-ABM-LAND 2应鉴定为N-葡萄糖吡喃-2-氨基苯甲酰胺，其中OMICRON-ABM-1应为β-异构体，OMICRON-ABM-2应为α-异构体。此外，OMICRON-ABM-3应鉴定为N-β-异麦芽糖基-2-氨基苯甲酰胺。上述结果表明，水稻植株选择性地吸收2-氨基苯甲酰胺作为相应的糖苷积累。结果表明，2-氨基苯胺草胺的相关化合物邻氨基苯甲酸也能被水稻吸收。但大米中邻氨基苯甲酸的糖基化效率低于2-氨基丁酰胺。我们推测，邻氨基苯甲酸糖基化效率低是因为它消耗了色氨酸的生物合成。目前还不清楚为什么水稻更喜欢吸收2-氨基苯甲酰胺并将其转化为相应的葡萄糖苷。

It is well known that plants use inorganic elements took from soil via root for their metabolisms. On the other hands a several of small organic compounds such as metabolites of soil microorganisms or artificial pollutants also present around plant rhizosphere. Effects of those organic compounds on plants have been unclear. First, we studied on absorption of rhizospheric microbial metabolites by rice plants to clarify a relationship between rhizospheric microorganisms mediated by organic compounds. We clarified rice plant prefers to absorb 2-aminobenzamide that is one of major metabolites of an actynomyces. To clarify a dynamic of absorbed 2-aminobenzamide, we performed purification and Identification of Its derivatives from rice plant. We extracted rice root with methanol to obtained remarked compound named omicron-ABm-1 omicron -ABm-2, omicron-ABzn-3 that could be thought to be metabolites from 2-aminobenzamide. Further spectroscopic analyses of those compounds revealed that omicron-ABm- land 2 should be identified to be N-glucopyranosil-2-aminobenzamide, in detail, omicron-ABm-1 should be beta-anomer and omicron-ABm-2 should be alfa-anomer. Additionally, omicron-ABm-3 should be identified to be N-beta-isomaltosylosil-2-aminobenzamide. Above result appeared that rice plant selectively absorbed 2-aminebenzamide to accumulate it as a corresponding glucoside. We obtained the result that anthranilic acid that is related compound of 2-aminobennzamode be also absorbed by rice plant. However glucosylation efficiency of anthranilic acid in rice was lower than it of 2- aminobeuzamide. We guess that low efficiency of anthranilic acid glucosylation caused by its consumption for tryptophan biosynthesis. It is unclear why rice plant prefer to absorb 2-aminobennzamide and convert it to its corresponding glucoside.