Effects of IgE and complement on the protective immunity against helimth infections.

为了分析对蠕虫感染的保护性免疫，研究了IgE和补体的作用。IgE缺乏小鼠的寄生虫感染：选择小黑门绦虫和日本血吸虫作为诱导抗寄生虫IgE抗体产生的寄生虫。用IgE缺乏小鼠SJA/9和IgE产生对照组SJL/J比较IgE的作用。在血吸虫感染中，IgE抗体对嗜酸性粒细胞无影响，但对成虫的排出有部分影响。在日本血吸虫感染中，IgE抗体似乎与肝脏中鸡蛋肉芽肿的形成部分相关。选择巴西尼波圆线虫和马来布鲁贾线虫作为诱导增强ige产生的蠕虫。SJL/J感染小鼠的总IgE水平比未感染小鼠高10倍，而SJA/9小鼠未检测到总IgE水平。两种小鼠感染后均未检测到抗甲型h1n1流感病毒IgE抗体。SJA/9和SJL/J对嗜酸性粒细胞和保护性免疫的影响不显著，说明增强的IgE对嗜酸性粒细胞和保护性免疫没有影响。这些事实表明，除IgE外，其他因素在蠕虫感染宿主嗜酸性粒细胞和保护性免疫中起主要作用。Clq对抗体依赖性细胞毒性（antibody dependent cellular cytotoxicity， ADCC）的影响：Clq以免疫双丝蚴微丝为靶点，IgE抗体和多态核白细胞为效应细胞，增强ADCC反应。这种增强的机制被认为是Clq分子尾部区域激活效应细胞和Clq头部区域增加IgE抗体结合。在后一种情况下，Clq明显增强了低亲和力抗体与抗原的结合。在高亲和力单克隆抗体存在的情况下，Clq增强了低亲和力抗半抗原IgE单克隆抗体与半抗原的结合，证实了这一发现。这些结果表明，Clq可能在感染后的早期阶段，在存在相对大量的低亲和力抗体的情况下增强保护性免疫。少

In order to analyse the protective immunity agagainst helminth infections, effects of IgE and complement were investigated.1. Heleminth infections in IgE deficient mice: Heymenolepis nana and Schistosoma japonicum were selected as the parasites inducing anti-parasite IgE antibody production. IgE deficient SJA/9 mice and IgE producing control SJL/J mice were used to compare the effects of IgE. In H.nana infection, IgE antibody had no effect on blood eosinophilia, but had partial effect on expulsion of adult worms. In S. japonicum infection, IgE antibody seems to relate partially to the egg granuloma formation in the liver. Nippostrongylus brasiliensis and Brugia malayi were selected as the helminths inducing potentiated ige production. total IgE level in infected mice increased 10 times higher than uninfected level in SJL/J, but did not detected in SJA/9 mice. Anti-hel-minth IgE antibody was not detected in both mice after infection. Blood eosinophilia and protective immunity were not s … More ignificantly different between SJA/9 and SJL/J, suggesting that potentiated IgE had no effect on eosinophilia and protective immunity. These facts indicate that factors other than IgE are mainly operative on eosinophilia and protective immunity in the hosts with helminth infection.2. Effects of Clq on antibody dependent cellular cytotoxicity(ADCC): Clq enhanced ADCC reaction with microfilaria of dilofilaria immitis as a target, IgE antibody, and polymorph nuclear leukocytes as effector cells. Mechanisms of this enhancement were considered to be activation of effector cells by tail region of Clq molecules and increase of IgE antibody binding by head region of Clq. In latter case, it was evident that binding of low avidity antibodies to antigen was enhanced by Clq. This finding was confirmed by the system where binding of low affinity anti-hapten IgE monoclonal antibody to the hapten was enhanced by Clq in the presence of high affinity monoclonal antibody. These results suggest the possibility that Clq enhances protective immunity in the early phase after infection at the presence of relatively large amount of low avidity antibodies. Less