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Effects of IgE and complement on the protective immunity against helimth infections.

IgE 和补体对蠕虫感染保护性免疫的影响。

基本信息

批准号：
62480150
负责人：
KOBAYASHI Akio
金额：
$ 3.14万
依托单位：
Jikei University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480150/
关键词：
Protective immunity helminth infection IgE Complement C1q Antibody dependent cellular cytotoxicity 好酸球増多 C_1q IgG 好酸球 Nippostrongylus 旋毛虫日本住血吸虫犬糸状虫

项目摘要

In order to analyse the protective immunity agagainst helminth infections, effects of IgE and complement were investigated.1. Heleminth infections in IgE deficient mice: Heymenolepis nana and Schistosoma japonicum were selected as the parasites inducing anti-parasite IgE antibody production. IgE deficient SJA/9 mice and IgE producing control SJL/J mice were used to compare the effects of IgE. In H.nana infection, IgE antibody had no effect on blood eosinophilia, but had partial effect on expulsion of adult worms. In S. japonicum infection, IgE antibody seems to relate partially to the egg granuloma formation in the liver. Nippostrongylus brasiliensis and Brugia malayi were selected as the helminths inducing potentiated ige production. total IgE level in infected mice increased 10 times higher than uninfected level in SJL/J, but did not detected in SJA/9 mice. Anti-hel-minth IgE antibody was not detected in both mice after infection. Blood eosinophilia and protective immunity were not s … More ignificantly different between SJA/9 and SJL/J, suggesting that potentiated IgE had no effect on eosinophilia and protective immunity. These facts indicate that factors other than IgE are mainly operative on eosinophilia and protective immunity in the hosts with helminth infection.2. Effects of Clq on antibody dependent cellular cytotoxicity(ADCC): Clq enhanced ADCC reaction with microfilaria of dilofilaria immitis as a target, IgE antibody, and polymorph nuclear leukocytes as effector cells. Mechanisms of this enhancement were considered to be activation of effector cells by tail region of Clq molecules and increase of IgE antibody binding by head region of Clq. In latter case, it was evident that binding of low avidity antibodies to antigen was enhanced by Clq. This finding was confirmed by the system where binding of low affinity anti-hapten IgE monoclonal antibody to the hapten was enhanced by Clq in the presence of high affinity monoclonal antibody. These results suggest the possibility that Clq enhances protective immunity in the early phase after infection at the presence of relatively large amount of low avidity antibodies. Less

为了分析抗蠕虫感染的保护性免疫，研究了IgE和补体的作用.在IgE缺陷小鼠中的Heleminth感染：选择微小黑壳绦虫（Heymenolepis nana）和日本血吸虫（Schistosoma japonicum）作为诱导抗寄生虫IgE抗体产生的寄生虫。使用IgE缺陷型SJA/9小鼠和产生IgE的对照SJL/J小鼠来比较IgE的作用。在微小血吸虫感染中，IgE抗体对血嗜酸性粒细胞无影响，但对蠕虫排出有部分影响。In S.日本血吸虫感染后，IgE抗体与肝虫卵肉芽肿的形成有一定关系。选择巴西日本圆线虫和马来丝虫作为诱导增强ige产生的蠕虫。SJL/J感染小鼠血清总IgE水平比未感染小鼠高10倍，而SJA/9小鼠未检测到。感染后两只小鼠均未检测到抗蠕虫IgE抗体。血嗜酸性粒细胞增多和保护性免疫未被发现。 ...更多信息 SJA/9和SJL/J之间有显著差异，表明增强的IgE对嗜酸性粒细胞和保护性免疫没有影响。这些事实表明，在蠕虫感染宿主中，除IgE外的其他因素主要对嗜酸性粒细胞增多和保护性免疫起作用. Clq对抗体依赖性细胞毒性（ADCC）的影响：Clq增强以犬恶丝虫微丝蚴为靶、IgE抗体和多形核白细胞为效应细胞的ADCC反应。这种增强的机制被认为是通过Clq分子的尾区激活效应细胞和通过Clq分子的头区增加IgE抗体结合。在后一种情况下，显然低亲合力抗体与抗原的结合被Clq增强。这一发现通过在高亲和力单克隆抗体存在下通过Clq增强低亲和力抗半抗原IgE单克隆抗体与半抗原的结合的系统得到证实。这些结果表明，在存在相对大量的低亲合力抗体的情况下，Clq在感染后的早期阶段增强保护性免疫的可能性。少