权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular Systematics of Eupatorieae in New World

新大陆紫茎泽兰的分子系统学

基本信息

批准号：
03041028
负责人：
YAHARA Tetsukazu
金额：
$ 5.44万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03041028/
关键词：
Eupatorium Molecular Systematics RFLP Chloroplast DNA Polyploidy 染色体数ヒヨドリバナ連

项目摘要

In Eupatorieae, n=10 is prevailing and has been considered to be a basic chromosome number. However, some genera have n=15,16,17 and 25. Based on evidence of extensive gene duplications in Eupatorium with n=10,Yahara et al (1991) postulated that the tribe is of polyploid origin and n=10 is derived from larger chromosome numbers. To test this hypothesis, We carried out molecular systematic study on genera with n=9,10,11,17, and 25.To obtain plant materials for molecular systematic studies, we carried out field works in Texas and Costa Rica. Total DNAs were isolated from these materials and RFLPs were detected using probes of lettuce chloroplast DNA. Clibadium and Coreopsis of Heliantheae were used as outgroups to construct a cladogram.As a result of cladistic on RFLP data sets, it is shown that two clades were diverged after the origin of the Eupatorieae ; one including Ageratina (n=17) and Neomirandia (n=17,25), and the other including Mikania (n=16-18),Stevia (n=11), eight genera with n=10, and Brickellia (n=9). This result supports the hypothesis that n=10 is derived from larger chromosome numbers. It is already shown from cpDNA data that Eupatorieae is derived from Heliantheae. In Heliantheae, it has been suggested that genera with n=16 in Heliantheae are derived from ancestral genera with n=8. The result of this study suggests that Eupatorieae isderived from species with n=16 in Heliantheae. In Eupatorieae, some Mexican genera have been known to have n=16. Materials of one of those genera, Bartlettina, have been recently obtained and molecular systematic analysis of this genus is now ongoing.

在紫茎泽兰中，n=10 很普遍，并被认为是基本染色体数。然而，一些属的n=15、16、17和25。基于在n=10的紫茎泽兰中广泛的基因重复的证据，Yahara等人（1991）推测该部落是多倍体起源，并且n=10源自较大的染色体数目。为了验证这一假设，我们对n=9、10、11、17和25的属进行了分子系统研究。为了获得用于分子系统研究的植物材料，我们在德克萨斯州和哥斯达黎加进行了实地工作。从这些材料中分离出总 DNA，并使用生菜叶绿体 DNA 探针检测 RFLP。以Heliantheae中的Clibadium和Coreopsis为外群构建了分支图。RFLP数据集的分支分析结果表明，在Eupatorieae起源之后，两个分支发生了分化；一类包括 Ageratina (n=17) 和 Neomirandia (n=17,25)，另一类包括薇卡尼亚 (n=16-18)、甜叶菊 (n=11)、8 个属，n=10 和 Brickellia (n=9)。该结果支持 n=10 源自较大染色体数目的假设。 cpDNA数据已经表明，紫茎泽兰科(Eupatorieae)源自向日葵科(Heliantheae)。在向日葵科中，有人提出，向日葵科中n=16的属源自n=8的祖先属。本研究结果表明，紫茎泽兰科植物源自向日葵科中n=16的物种。在紫茎泽兰科中，已知一些墨西哥属的 n=16。最近获得了其中一个属 Bartlettina 的材料，目前正在进行该属的分子系统分析。