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Immunohistochemical Analysis of Odontogenic and Osteogenic Tumors

牙源性和骨源性肿瘤的免疫组织化学分析

基本信息

批准号：
03304041
负责人：
NAGAI Noriyuki
金额：
$ 3.26万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

Odontogenic tumors are thought to be originated from epithelial and /or mesenchymal tissue of tooth germ, because of morphological similarities between these tumors and tooth germs. But the origin and histogenesis of most of the odontogenic tumors have been remained to be unproven. Amelogenin, an organic matrix of enamel in the early developmental stage, is well known to be proline rich glycoprotein with low molecular weight, distinct from keratins or collagen molecules(10-11). Since complete amino acid sequence of amelogenin was accomplished by Takagi et al (12) and Yeh et al (13), non cross-reacting monoclonal antibodies against amelogenin were developed. With these monoclonal antibodies, amelogenin was detected ultrastructurally, and merocrine secretion of amelogenin by late preameloblasts and secretory ameloblasts was elucidates. The detection of amelogenin was considered to be useful as a marker of functioning ameloblasts in clarifying the histogenesis of odontogenic tumors.Immuno … More histochemical expressions of cytokeratin, amelogenin, and enamelin were studies in odontogenic tumors; ameloblastoma, adenomatoid odontogenic tumor, ameloblastic carcinoma. Four anti-cytokeratin monoclonal antibodies were used in this study: CK-1 to total keratin, SE-K to 56,56.5,58 and 68Kd,NSE-K to 52.5Kd,and 19K to 40Kd. Cytokeratin expression in follicular ameloblastoma was similar to that in tooth germ epithelia, and in plexiform ameloblastoma similar to that in fetal oral mucosa. Both types of ameloblastoma also showed slight immunoreactivity for amelogenin. In adenomatoid odontogenic tumor there was a difference between cytokeratin expression in central cells and in peripheral cells in the tumor nests. Moreover, cytokeratin expression in adenomatoid odontogenic tumor was completely different from those in ameloblastoma and tooth germ. Immunoreactivity for amelogenin and enamelin in adenomatoid odontogenic tumor was found in colloidal drops, calcified masses and lining cell membrane of ductlike structure. Therefore, it is possible that these tumor cells differentiate into enamel protein-producing cells, more than those of ameloblastoma do. Immunohistochemical results in ameloblastic carcinoma showed that this carcinoma is composed of undifferentiated odontogenic tumor cells. Also, an immunohistochemical study of localization of type I,II and III collagen were discussed in mandibular condylar cartilage compared with fibrillar bone. Bone matrix protein detected in osteogenic tumors. Less

牙源性肿瘤被认为是起源于牙胚的上皮和/或间充质组织，因为这些肿瘤与牙胚之间的形态相似。但绝大多数牙源性肿瘤的起源和组织发生至今仍不清楚。釉原蛋白是早期发育阶段釉质的有机基质，众所周知是富含脯氨酸的低分子量糖蛋白，不同于角蛋白或胶原蛋白分子（10-11）。由于Takagi等（12）和Yeh等（13）完成了釉原蛋白的完整氨基酸序列，因此开发了针对釉原蛋白的非交叉反应单克隆抗体。用这些单克隆抗体对釉原蛋白进行了超微结构检测，并阐明了晚期成釉细胞和分泌型成釉细胞的釉原蛋白分泌。釉原蛋白的检测被认为是一个有用的标记功能成釉细胞在阐明牙源性肿瘤的组织发生。 ...更多信息本研究应用组织化学方法，对牙源性肿瘤、成釉细胞瘤、牙源性腺瘤样瘤、成釉细胞癌中细胞角蛋白、釉原蛋白和釉蛋白表达进行了研究。用CK-1、SE-K、NSE-K、19 K和19 K四种单克隆抗体分别对总角蛋白、56、56.5、58和68 Kd、52.5Kd和40 Kd进行了检测。细胞角蛋白在滤泡型成釉细胞瘤中的表达与牙胚上皮相似，在丛状型成釉细胞瘤中的表达与胎儿口腔黏膜相似。两种类型的成釉细胞瘤也表现出轻微的釉原蛋白免疫反应。在牙源性腺瘤样瘤中，癌巢中央细胞和周围细胞角蛋白表达存在差异。此外，细胞角蛋白在牙源性腺瘤样瘤中的表达与成釉细胞瘤和牙胚中的表达完全不同。牙源性腺瘤样瘤中釉原蛋白和釉蛋白的免疫反应主要见于牙源性腺瘤样瘤中的胶滴、钙化块及导管样结构的衬膜。因此，这些肿瘤细胞可能比成釉细胞瘤细胞更能分化成釉蛋白产生细胞。成釉细胞癌的免疫组化结果表明，这种癌是由未分化的牙源性肿瘤细胞。此外，还讨论了I型、II型和III型胶原蛋白在下颌髁突软骨中定位的免疫组织化学研究，并与纤维骨进行了比较。成骨肿瘤中检测到的骨基质蛋白。少