Immunohistochemical Analysis of Odontogenic and Osteogenic Tumors

牙源性肿瘤被认为起源于牙胚的上皮和/或间充质组织，因为这些肿瘤与牙胚的形态相似。但大多数牙源性肿瘤的起源和组织发生仍未得到证实。釉原蛋白是釉质发育早期的一种有机基质，是一种低相对分子质量的富含脯氨酸的糖蛋白，不同于角蛋白或胶原分子(10-11)。由于Takagi等人(12)和Yeh等人(13)完成了釉原蛋白的完整氨基酸序列，因此研制了无交叉反应的抗釉原蛋白单抗。用这些单抗对釉原蛋白进行了超微结构的检测，并阐明了晚期前成釉细胞和分泌性成釉细胞分泌釉原蛋白的情况。成釉蛋白的检测被认为是功能成釉细胞的标志，有助于阐明牙源性肿瘤的组织起源。免疫…在牙源性肿瘤、成釉细胞瘤、腺瘤样牙源性肿瘤、成釉细胞癌中有更多的细胞角蛋白、釉原蛋白和釉蛋白的组织化学表达。本研究使用了四种抗细胞角蛋白的单抗：CK-1至总角蛋白，SE-K至56，56.5，58及68Kd，NSE-K至52.5Kd，19K至40Kd。滤泡性成釉细胞瘤中细胞角蛋白的表达与牙胚上皮相似，丛状成釉细胞瘤中细胞角蛋白的表达与胎儿口腔黏膜相似。两种类型的成釉细胞瘤对釉原蛋白也呈轻度免疫反应。在腺瘤样牙源性肿瘤中，瘤巢中央细胞和周围细胞角蛋白的表达存在差异。此外，细胞角蛋白在牙源性腺瘤中的表达与在成釉细胞瘤和牙胚中的表达完全不同。在腺瘤样牙本质瘤中，釉原蛋白和釉蛋白免疫反应主要见于胶体滴、钙化肿块和导管样结构的衬里细胞膜。因此，这些肿瘤细胞有可能比成釉细胞瘤更多地分化为釉质蛋白产生细胞。成釉细胞癌的免疫组织化学结果显示，该癌由未分化的牙源性肿瘤细胞组成。此外，还讨论了I、II和III型胶原在下颌骨软骨和纤维骨中定位的免疫组织化学研究。在成骨肿瘤中检测到骨基质蛋白。较少

Odontogenic tumors are thought to be originated from epithelial and /or mesenchymal tissue of tooth germ, because of morphological similarities between these tumors and tooth germs. But the origin and histogenesis of most of the odontogenic tumors have been remained to be unproven. Amelogenin, an organic matrix of enamel in the early developmental stage, is well known to be proline rich glycoprotein with low molecular weight, distinct from keratins or collagen molecules(10-11). Since complete amino acid sequence of amelogenin was accomplished by Takagi et al (12) and Yeh et al (13), non cross-reacting monoclonal antibodies against amelogenin were developed. With these monoclonal antibodies, amelogenin was detected ultrastructurally, and merocrine secretion of amelogenin by late preameloblasts and secretory ameloblasts was elucidates. The detection of amelogenin was considered to be useful as a marker of functioning ameloblasts in clarifying the histogenesis of odontogenic tumors.Immuno … More histochemical expressions of cytokeratin, amelogenin, and enamelin were studies in odontogenic tumors; ameloblastoma, adenomatoid odontogenic tumor, ameloblastic carcinoma. Four anti-cytokeratin monoclonal antibodies were used in this study: CK-1 to total keratin, SE-K to 56,56.5,58 and 68Kd,NSE-K to 52.5Kd,and 19K to 40Kd. Cytokeratin expression in follicular ameloblastoma was similar to that in tooth germ epithelia, and in plexiform ameloblastoma similar to that in fetal oral mucosa. Both types of ameloblastoma also showed slight immunoreactivity for amelogenin. In adenomatoid odontogenic tumor there was a difference between cytokeratin expression in central cells and in peripheral cells in the tumor nests. Moreover, cytokeratin expression in adenomatoid odontogenic tumor was completely different from those in ameloblastoma and tooth germ. Immunoreactivity for amelogenin and enamelin in adenomatoid odontogenic tumor was found in colloidal drops, calcified masses and lining cell membrane of ductlike structure. Therefore, it is possible that these tumor cells differentiate into enamel protein-producing cells, more than those of ameloblastoma do. Immunohistochemical results in ameloblastic carcinoma showed that this carcinoma is composed of undifferentiated odontogenic tumor cells. Also, an immunohistochemical study of localization of type I,II and III collagen were discussed in mandibular condylar cartilage compared with fibrillar bone. Bone matrix protein detected in osteogenic tumors. Less