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The Analysis of Gene Expression of Morphogenetic Factors Implicated Development of Tooth Germ and Odontogenic Tumors

牙胚发育及牙源性肿瘤形态发生因子基因表达分析

基本信息

批准号：
09470391
负责人：
NAGAI Noriyuki
金额：
$ 6.78万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Tooth morphogenesis and cytodifferentiations are regulated by epithelium-mesenchymal interactions, and the basement membrane plays a role in mediating these interactions Type IV collagen, the major component of the dental basement membrane, is composed of three αchains. Using indirect immunofluorescence on cryosections, we characterized the changes in protein localization of α1 to α6 chains of type IV collagen during mouse molar development. We propose that the inner enamel epithlium in the cap stage is composed of type IV collagen consist of α1, α2 and α4 chains, probably in molecular forms of α1(IV) α2(IV) α4(IV), [α4(IV)]ィイD23ィエD2 and [α1(IV)]ィイD22ィエD2 α2(IV). These forms containing α4 chain have not been detected in other organs yet. Therefore, it is thought that these forms is related to morphogenesis and cytodifferentiation characteristic of tooth development that regulated y epithelial-mesenchymal interactions.Recently, it has gradually been ascertained that some growth factors secreted by epithelial or mesenchymal tissue bind to type IV collagen with greater affinity, condens there and contribute to organ morphogenesis in cooperation with basement membrane. We observed the expression patterns of several growth factors (TGF-β, BMPs and FGFs) in the basement menbrane of mouse first molar during tooth germ development and explored the relation between these growth factors and the tooth germ-specific molecular forms of type IV collage. No examined growth factor was detected in the basement membrane in and after cap stage. On the other hand, in the dental lamina and bud stages FGF-2 and TGF-β1 were detected in the dental basement membrane. FGF-2 and TGF-β1 may play an important role in early tooth morphogenesis and cytodifferentiation in cooperation with the molecular form, α3(IV)α4(IV)α5(IV), which exsists in the early stage of tooth development.

牙齿形态发生和细胞分化受上皮-间充质相互作用的调节，基底膜在介导这些相互作用中起作用。IV型胶原是牙齿基底膜的主要成分，由三条α链组成。利用冷冻切片上的间接免疫荧光，我们表征了小鼠磨牙发育过程中IV型胶原α1至α6链蛋白定位的变化。我们认为帽状期内釉上皮是由α_1、α_2和α_4链组成的Ⅳ型胶原，可能以α_1（IV）α_2（IV）α_4（IV）、[α_4（IV）] β_23 β_2D_2和[α_1（IV）] β_22 β_2D_2 α_2（IV）的分子形式存在。这些含有α4链的形式尚未在其他器官中检测到。因此，这些形式被认为与调节上皮-间充质相互作用的牙齿发育的形态发生和细胞分化特征有关。最近，已经逐渐确定，上皮或间充质组织分泌的一些生长因子以更大的亲和力与IV型胶原结合，在那里浓缩，并与基底膜合作促进器官形态发生。本研究观察了几种生长因子（TGF-β、BMPs和FGFs）在小鼠第一磨牙牙胚发育过程中基底膜的表达规律，并探讨了这些生长因子与牙胚特异性IV型胶原分子形态的关系。帽状期及帽状期后，在基底膜中未检测到受检生长因子。在牙板期和牙胚期，FGF-2和TGF-β1在牙体基底膜中表达。FGF-2和TGF-β1可能与存在于牙齿发育早期的分子形式α3（IV）α4（IV）α5（IV）协同作用，在牙齿的早期形态发生和细胞分化中发挥重要作用。