Bone marrow stromal cells concerning with jaw-bone reconstruction and a role of microenviroment

老年社会开始的最重要的主题之一是由于牙齿缺失而导致的咬合功能丧失。为了恢复文化舒适的生活，有几种治疗方法可以恢复咬合功能的丧失，即颌骨重建和种植牙。在选择这些方法和适用病例之前，开发控制和诱导颌骨细胞向成骨细胞分化的系统是重要的。第一年：我们研究了羟基磷灰石表面结构与细胞反应之间的关系。我们揭示了以下事实：（1）成骨细胞的细胞分化和巨噬细胞的出现之间存在耦合机制（Shirasuga等）。(2)大分子溶解其核糖体中的磷灰石（永井）。(3)骨强直（骨接触，骨整合）是必要的，以稳定的牙种植体在颌骨（赤城）。第2年：我们检查的事实，影响他们在体外研究的多种方式。我们使用的系统是：磷灰石-成骨细胞（HOS），磷灰石-骨膜在培养的骨髓基质细胞的多潜能分化（Takesita，Ono，Tsuji）。结果表明，磷灰石在界面处起着基质材料的作用，并与矿化有关。微环境控制成骨细胞的分化，提示：（1）微环境（PH、Ca^2+浓度）与骨髓基质细胞的成骨分化和矿化有关;（2）磷灰石也与这些动力学方面存在正相关。

One of the most important theme in commencing aged society is a loss of occulsal function due to the missing of teeth. There are several treatments to be restored the loss of occulsal function in order to recover a cultural comfortable life, that is, reconstruction of jaw bone and dental implant. It is important to develop the systems to control and induce the osteoblastic differentiation of jaw bone cells before we select these methods and adaptable cases.Year 1: We investigated relations between a surface structure of hydroxy-apatite and cell responses to it. We revealed the following facts; (1) There is a coupling mechanism between a cytodifferentiation of osteoblastic cells and an appearance of macrophages (Shirasuga et al). (2) Macrophages dissolve apatite in their risosomes (Nagai). (3) Osteo-ankylosis (osseocontact, osseointegrate) is necessary to stabilize the dental implants in jaw bone (Akagi).Years 2: We examined the facts which influenced on them by in vitro studies in the multiple ways. Systems which we used were; apatite-osteogenic cells(HOS), apatite-periostium in culture bone marrow stromal cells of multi potential differentiation (Takesita,Ono,Tsuji). We revealed that apatite played a role of matrix material and related to mineralization at the interface. Microenviroment controlled the cytodifferntiation of osteoblastic cells.From these facts, it was suggested that (1)microenviroment (PH,Ca^<2+> concentration) were related to osteogenic differentiation and mineralization of bone marrow stromal cells. (2)Apatite were also positively concerning with these dynamic aspects.