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Expression of Cytokeratins in Odontogenic Tumors.

细胞角蛋白在牙源性肿瘤中的表达。

基本信息

批准号：
03807120
负责人：
NAGAI Noriyuki
金额：
$ 1.15万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

Cytokeratins, intermediate filaments characteristic of epithelial cells, are well known to be closely related to epithelial differentiation. That is, the differentiation specificity to expression of different composition of cytokeratins has been described in epithelial cell types. Along these approaches, the expression of cytokeratins has been observed in the odontogenic epithelium and analyzed with special reference to the relationship between tooth germs and odontogenic cysts and tumors. But the distribution of cytokeratins has been unlikely to explain satisfactorily the origin of odontogenic tumors, because of heterogenous expression of cytokeratins in odontogenic tumors.1.Ameloblastoma ; Interestingly, they presented difference of cytokeratin distribution between follicular and plexiform types. In follicular ameloblastomas, the central portion showed immunoreactivity with all markers of cytokeratins, SE-K, NSE-K and K19. But the columnar cells arranged in the periphery were reacted … More positively for K19, faintly reacted for NSE-K, and negatively responded for the marker of squamous cells, SE-K. In plexiform ameloblastoma both central and peripheral cells demonstrated the positive reaction with all markers, SE-K, NSE-K and K19.2.Adenomatoid Odontogenic Tumors ; Adenomatoid odontogenic tumors demonstrated different distribution between central areas composed of columnar cells and peripheral areas of small round cells. Most areas of central cells, showing whorled structure or solid nests, demonstrated no expression for the marker of squamous cells, SE-K, however, only a few areas showed faint positivity. The immunoreactivity for NSE-K and K19 was so heterogenous that both immunoreactive and non-reactive areas were present within the central area. Ductal or tubular structures also showed no immunoreactivity for the marker of squamous cells (SE-K) with positive reaction for NSE-K and K19. The cells surrounding central whorled structures were smaller than central columnar cells and were proliferated without organizing pattern. These peripheral cells showed positive reaction for all markers of cytokeratins. Areas of reticular pattern composed of small round cells, which was frequently found in adenomatoid odontogenic tumors, also showed the same cytokeratin distribution with peripheral cells.3.Enamel Organ ; Along the maturation to enamel organ from dental lamina, the immunoreaction for the marker of squamous differentiation, SE-K, was negatively reacted. NSE-K and K19 were well demonstrated in the enamel organ proper. Less

细胞角蛋白是上皮细胞特有的中间纤维，与上皮细胞分化密切相关。也就是说，在上皮细胞类型中已经描述了对细胞角蛋白的不同组成的表达的分化特异性。沿着这些方法，细胞角蛋白在牙源性上皮中的表达已被观察到，并分析了牙胚与牙源性囊肿和肿瘤之间的关系。但由于角蛋白在牙源性肿瘤中的表达具有异质性，因此角蛋白的分布并不能很好地解释牙源性肿瘤的起源。1.成釉细胞瘤：有意思的是，滤泡型和丛状型的角蛋白分布存在差异。在滤泡性成釉细胞瘤中，中央部分与细胞角蛋白、SE-K、NSE-K和K19的所有标记物均呈免疫反应性。而排列在周围的柱状细胞则有反应 ...更多信息 K19阳性，NSE-K弱阳性，鳞状细胞标志物SE-K阴性。丛状型成釉细胞瘤的中央和外周细胞与SE-K、NSE-K和K19均呈阳性反应。2.牙源性腺瘤样瘤：牙源性腺瘤样瘤中央区由柱状细胞组成，外周区由小圆形细胞组成。鳞状细胞标志物SE-K在中央细胞呈螺旋状或实巢状的大部分区域不表达，但仅少数区域呈弱阳性。NSE-K和K19的免疫反应是如此异质性，免疫反应和非反应区都存在于中央区。导管或管状结构也未显示鳞状细胞标记物（SE-K）的免疫反应性，NSE-K和K19呈阳性反应。中央轮状结构周围的细胞比中央柱状细胞小，增殖无规律。这些细胞对所有细胞角蛋白标记物均呈阳性反应。牙源性腺瘤样瘤中常见的由小圆形细胞组成的网状区也显示与周围细胞相同的细胞角蛋白分布。3.釉器：沿着牙板向釉器的成熟，鳞状分化标志物SE-K免疫反应呈阴性。NSE-K和K19在成釉器中表达较好。少