Changes in lactotroph responsiveness to secretagogues as determined by the sequential cell immunoblot assay

通过序贯细胞免疫印迹测定确定泌乳素对促分泌素反应性的变化

• 批准号: 03670081
• 负责人: ARITA Jun
• 金额: $ 1.22万
• 依托单位: Yamanashi Medical University (1993)Yokohama City University (1991-1992)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670081/
• 关键词: Anterior pituitary gland; Prolactin; Dopamine; TRH; Estrogen; Sequential cell immunoblot assay; 甲状腺刺激ホルモン放出ホルモン; ド-パミン

We investigated heterogeneity for the responsiveness of lactotrophs to secretagogues and the effects of estradiol on the responsiveness by quantifying prolactin secretion at the single cell level.1. Heterogeneity for the responsiveness of lactotrophs to thyrotropin-releasing hormone(TRH)(1) Although prolactin secretion from single lactotrophs in culture was increased by TRH in a dose-dependent manner, approximately 50% of total lactotrophs were not stimulated by TRH even at a maximally effective dose. This result suggests that there are two subpopulations of lactotrophs that are responsive and unresponsive to TRH.(2) A population of lactotrophs that secrete small amounts of prolactin under basal conditions contains a much larger proportion of TRH-responsive lactotrophs than does a population of lactotrophs that secrete large amounts of prolactin.2. Changes by estradio1 in the responsiveness of lactotrophs to secretagogues(1) When the effects of estradiol treatment on prolactin secretion from a population of lactotrophs to medium were examined, it was found that estradiol increased basal secretion of prolactin. Estradiol attenuated and enhanced the inhibitory action of dopamine and the stimulatory action of TRH, respectively, on prolactin secretion.

我们通过在单细胞水平上定量催乳素分泌来研究催乳素对促分泌素反应的异质性以及雌二醇对反应的影响。促乳素细胞对促甲状腺素释放激素（TRH）反应性的异质性（1）尽管TRH以剂量依赖的方式增加培养中单个促乳素细胞的催乳素分泌，但即使在最大有效剂量下，约50%的总促乳素细胞也不被TRH刺激。这一结果表明，有两个亚群的催乳素是响应和不响应TRH。(2)在基础条件下分泌少量催乳素的催乳素细胞群比分泌大量催乳素的催乳素细胞群含有更大比例的TRH反应性催乳素细胞。雌二醇对催乳细胞对促分泌素反应性的影响（1）当研究雌二醇处理对催乳细胞群向培养基中分泌催乳素的影响时，发现雌二醇增加催乳素的基础分泌。雌二醇分别减弱和增强多巴胺对催乳素分泌的抑制作用和TRH对催乳素分泌的刺激作用。

项目成果

J.ARITA: "Heterogeneity in the secretory activity of pituitary lactotrophs revealed by analysis at the single cell level" YOKOHAMA MEDICAL BULLETIN. (1992)

J.ARITA：“单细胞水平分析揭示垂体催乳素分泌活性的异质性”《横滨医学通报》。

J.ARITA: "Analysis of the Secretion from Single Anterior Pituitary Cells by Cell Immunoblot Assay" Endocrine Journal. 40. 1-15 (1993)

J.ARITA：“通过细胞免疫印迹分析分析单个垂体前叶细胞的分泌物”内分泌杂志。

J.ARITA: "Heterogeneity in the Secretory Activity of Pituitary Lactotrophs Revealed by Analysis at the Single Cell Level" Yokohama Medical Bulletin. 43. 61-80 (1992)

J.ARITA：“单细胞水平分析揭示垂体泌乳素分泌活性的异质性”横滨医学通报。

J.ARITA: "Lactotrophs secreting small amounts of prolactin reveal great responsiveness to thyrotropin-releasing hormone" ENDOCRINOLOGY. 130. 3167-3174 (1992)

J.ARITA：“分泌少量催乳素的泌乳素显示出对促甲状腺素释放激素的强烈反应”内分泌学。

J.ARITA: "Lactotrophs Secreting Small Amounts of Prolactin Reveal Great Responsiveness to Thyrotropin-Releasing Hormone:Analysis by the Sequential Cell Immunoblot Assay" Endocrinology. 130. 3167-3174 (1992)

J.ARITA：“分泌少量催乳素的泌乳素显示出对促甲状腺素释放激素的巨大反应：通过连续细胞免疫印迹分析进行分析”内分泌学。