Conversion from stimulation to inhibition of the action of estrogen on cell proliferation

雌激素对细胞增殖的作用由刺激转为抑制

基本信息

  • 批准号:
    13670058
  • 负责人:
  • 金额:
    $ 2.3万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2001
  • 资助国家:
    日本
  • 起止时间:
    2001 至 2002
  • 项目状态:
    已结题

项目摘要

Studies using rat lactotrophs in primary culture in 20011 Estradiol (E2) stimulated basal proliferation of lactotrophs in primary culture with a long latency, 96 h, while it inhibited insulin-induced proliferation within 12 h. The ED50 were 8 and 85 pM for the E2 mitogenic and antimitogenic actions, respectively.2 This antimitogenic action was dependent upon the estrogen activity and not observed for androgens and progestins.3 In addition to the mitogenic action, the E2 antimitogenic action was antagonized by antiestrogens, indicating an estrogen receptor-mediated action.4 E2 inhibited not only insulin-induced lactotroph proliferation but also insulin-like growth factor-1 (IGF-1)-induced proliferation while it enhanced proliferation induced by serum or increased levels of intracellular cyclic AMP, suggesting that the E2 antimitogenic action is not dependent upon proliferative levels but mitogen-specific.Studies using prolactin-producing cell lines in 20021 In GH_3 cells, E2 inhibited IGF-1-induced proliferation and stimulated serum-induced proliferation as in lactotrophs in primary culture. However, E2 failed to stimulate basal proliferation of GH_3 cells.2 In another prolactin-producing cell line, GH_4C_1 cells, E2 inhibited IGF-1-induced proliferation and, unlike to GH_3 cells, stimulated basal proliferation.3 Double immunostaining for prolactin and the cell cycle-regulating protein cyclin D1 revealed that stimulation and inhibition of lactotroph proliferation induced by the mitogenic and antimitogenic actions of E2, respectively, were associated with parallel changes in the number of cyclin D1-immunoreactive lactotrophs.
20011年在原代培养中使用大鼠催乳细胞的研究表明,E2刺激原代培养中催乳细胞的基础增殖,潜伏期较长(96 h),而在12 h内抑制胰岛素诱导的增殖。E2促有丝分裂和抗有丝分裂作用的ED 50分别为8和85 pM。2这种抗有丝分裂作用依赖于雌激素活性,而雄激素和孕激素则未观察到。3除了促有丝分裂作用外,E2的抗有丝分裂作用被抗雌激素拮抗,表明雌激素受体介导的作用。4 E2不仅抑制胰岛素诱导的催乳细胞增殖,而且抑制胰岛素样生长因子-1(IGF-1)。E_2的抗有丝分裂作用不依赖于增殖水平,而依赖于有丝分裂原特异性。E2抑制IGF-1诱导的增殖,并刺激血清诱导的增殖,在原代培养的催乳细胞。在另一种催乳素产生细胞系GH_4C_1中,E2抑制IGF-1诱导的细胞增殖,与GH_3细胞不同,刺激基础增殖。3催乳素和细胞周期的双重免疫染色-细胞周期蛋白D1的调节揭示了促有丝分裂和抗有丝分裂作用诱导的催乳细胞增殖的刺激和抑制,E2分别与细胞周期蛋白D1免疫反应性催乳细胞数量的平行变化相关。

项目成果

期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
P. Yin: "Proestrous surge of gonadotropin-releasing hormone secretion inhibits apoptosis of anterior pituitary cells in cycling female rats"Neuroendocrinology. 75 (6). 272-282 (2002)
P. Yin:“促性腺激素释放激素分泌的发情前期激增抑制循环雌性大鼠垂体前叶细胞的凋亡”神经内分泌学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
K.Kawashima: "The estrogen-occupied estrogen receptor functions as a negative regulator to inhibit cell proliferation induced by insulin/IGF-1 :a cell context-specific antimitogenic action of estradiol on lactotrophs in culture"Endocrinology. 143(7). 2750
K.Kawashima:“雌激素占据的雌激素受体充当负调节剂,抑制胰岛素/IGF-1诱导的细胞增殖:雌二醇对培养物中催乳素细胞的细胞环境特异性抗有丝分裂作用”内分泌学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
K.Kawashima: "The estrogen-occupied estrogen receptor functions as a negative regulator to inhibit cell proliferation induced by insulin/IGE-1:a cell context-specific antimitogenic action of estradiol on rat lactotrophs in culture"Endocrinology. 143(7). 2
K.Kawashima:“雌激素占据的雌激素受体作为负调节因子抑制胰岛素/IGE-1诱导的细胞增殖:雌二醇对培养的大鼠泌乳素细胞的细胞环境特异性抗有丝分裂作用”内分泌学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
P.Yin: "Proestrous surge of gonadotropin-releasing hormone secretion inhibits apoptosis of anterior pituitary cells in cycling female rats"Neuroendocrinology. 76(5). 272-282 (2002)
P.Yin:“促性腺激素释放激素分泌的发情前激增抑制循环雌性大鼠垂体前叶细胞的凋亡”神经内分泌学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
P.Yin: "Direct actions of estradiol on the anterior pituitary gland are required for hypothalamus-dependent lactotrope proliferation and secetory surges of luteinizing hormone but not of prolactin female rats"Neuroendocrinology. 75(6). 392-401 (2002)
P.Yin:“雌二醇对垂体前叶的直接作用是下丘脑依赖性催乳素增殖和黄体生成素分泌激增所必需的,但催乳素雌性大鼠则不需要”神经内分泌学。
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  • 影响因子:
    0
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ARITA Jun其他文献

ARITA Jun的其他文献

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{{ truncateString('ARITA Jun', 18)}}的其他基金

Identification of the gene involved in the anti-proliferative action of estrogen
鉴定参与雌激素抗增殖作用的基因
  • 批准号:
    24590297
  • 财政年份:
    2012
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular mechanism for the interaction between estrogen receptor and G protein-coupled receptor
雌激素受体与G蛋白偶联受体相互作用的分子机制
  • 批准号:
    21590254
  • 财政年份:
    2009
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Rat strain difference in estrogen responsiveness of cell proliferation
大鼠品系对细胞增殖雌激素反应性的差异
  • 批准号:
    19590224
  • 财政年份:
    2007
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Role of the transcription factor CREB in the regulation of cell proliferation in estrogen-responsive cells
转录因子CREB在雌激素反应性细胞增殖调节中的作用
  • 批准号:
    17590198
  • 财政年份:
    2005
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Identification of estrogen-responsive cells involved in cell proliferation in response to estrogen
鉴定参与雌激素反应的细胞增殖的雌激素反应细胞
  • 批准号:
    15590206
  • 财政年份:
    2003
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Intracellular signal transduction pathways regulating proliferation of anterior pituitary cells
调节垂体前叶细胞增殖的细胞内信号转导通路
  • 批准号:
    10670060
  • 财政年份:
    1998
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Identification of the hypothalamic hormone that regulates proliferatin of lactorophs
调节乳酸菌增殖的下丘脑激素的鉴定
  • 批准号:
    08670081
  • 财政年份:
    1996
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Changes in lactotroph responsiveness to secretagogues as determined by the sequential cell immunoblot assay
通过序贯细胞免疫印迹测定确定泌乳素对促分泌素反应性的变化
  • 批准号:
    03670081
  • 财政年份:
    1991
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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