Role of the transcription factor CREB in the regulation of cell proliferation in estrogen-responsive cells
转录因子CREB在雌激素反应性细胞增殖调节中的作用
基本信息
- 批准号:17590198
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2005
- 资助国家:日本
- 起止时间:2005 至 2006
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1 We generated Ad-TRE/MCREB.Tag, an adenovirus vector expressing the dominant negative CREB mutant MCREB. We verified morphologically and functionally the expression of MCREB protein.2 Infection with Ad-TRE/bgal as a negative control did not change forskolin-induced proliferation of lactotrophs whereas Ad-TRE/MCREB.Tag infection completely blocked forskolin-induced proliferation.3 Infection with Ad-TRE/MCREB.Tag partially suppressed stimulation of lactotroph proliferation by insulin-like growth factor-1 (IGF-1), a mitogen whose action is mediated by a signaling pathway distinct from the cyclic AMP/protein kinase A pathway.4 Treatment with forskolin or estradiol alone increased prolactin promoter activity and their combination further increased the promoter activity. Infection with Ad-TRE/bgal did not change basal or forskolin-induced levels of prolactin promoter activity whereas Ad-TRE/MCREB.Tag infection partially suppressed the basal and forskolin-induced levels.5 Intracellular cyclic AMP levels were markedly increased by forskolin treatment but not by IGF-1 treatment. Forskolin treatment markedly increased the proportion of phosphorylated CREB-immunoreactive lactotrophs at 20 min and up to 9 h whereas IGF-1 treatment modestly increased it only at 20 min and 1 h.
1 我们生成了 Ad-TRE/MCREB.Tag,这是一种表达显性失活 CREB 突变体 MCREB 的腺病毒载体。我们从形态和功能上验证了 MCREB 蛋白的表达。2 作为阴性对照,Ad-TRE/bgal 感染不会改变毛喉素诱导的泌乳素细胞增殖,而 Ad-TRE/MCREB.Tag 感染完全阻断毛喉素诱导的增殖。3 Ad-TRE/MCREB.Tag 感染部分抑制了毛喉素诱导的泌乳素增殖刺激。 胰岛素样生长因子-1 (IGF-1) 是一种有丝分裂原,其作用由不同于环 AMP/蛋白激酶 A 途径的信号途径介导。4 单独使用毛喉素或雌二醇治疗会增加催乳素启动子活性,而它们的组合会进一步增加启动子活性。 Ad-TRE/bgal 感染不会改变基础或毛喉素诱导的催乳素启动子活性水平,而 Ad-TRE/MCREB.Tag 感染部分抑制基础和毛喉素诱导的水平。 5 细胞内环 AMP 水平通过毛喉素处理显着增加,但通过 IGF-1 处理则没有。 Forskolin 处理在 20 分钟和 9 小时内显着增加磷酸化 CREB 免疫反应性泌乳素的比例,而 IGF-1 处理仅在 20 分钟和 1 小时时适度增加。
项目成果
期刊论文数量(13)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Perinatal exposure to TCDD suppresses contextual fear conditioning-accompanied activation of cyclic AMP response element-binding protein in the hippocampal CAI region of male rats
围产期暴露于 TCDD 抑制雄性大鼠海马 CAI 区域中环境恐惧调节伴随的环 AMP 反应元件结合蛋白的激活
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Honma;K.;S.Hashimoto;T.Miyazaki;S.Honma.;T.Mitsui
- 通讯作者:T.Mitsui
Estrogen actions on lactotroph proliferaiton are independent of a paracrine interaction with other pituitary cell types : a stud using lactotroph-enriched cells.
雌激素对泌乳素增殖的作用独立于与其他垂体细胞类型的旁分泌相互作用:一项使用富含泌乳素的细胞的研究。
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Yamanaka;Y.;K.Honma;S.Hashimoto;N.Takatsu;T.Miyazaki;S.Honma.;M.Ishida et al.
- 通讯作者:M.Ishida et al.
Pup removal suppresses estrogen-induced surges of LH secretion and activation of GnRH neurons in lactating rats.
去除幼崽可抑制哺乳期大鼠雌激素诱导的 LH 分泌激增和 GnRH 神经元激活。
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Honma;K.;S.Masubuchi;S.Hashimoto;T.Endo;S.Honma.;A.Fukushima et al.
- 通讯作者:A.Fukushima et al.
Pup removal suppresses estrogen-induced surges of LH secretion and activation of GnRH neurons in lactating rats
去除幼仔可抑制雌激素诱导的哺乳期大鼠 LH 分泌激增和 GnRH 神经元激活
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Hasegawa I;Niisato N;A.Fukushima
- 通讯作者:A.Fukushima
Estrogen actions on lactotroph proliferaiton are independent of a paracrine interaction with other pituitary cell types : a study using lactotroph-enriched cells.
雌激素对泌乳素增殖的作用独立于与其他垂体细胞类型的旁分泌相互作用:一项使用富含泌乳素的细胞的研究。
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Yamanaka;Y. et al.;M.Ishida
- 通讯作者:M.Ishida
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
ARITA Jun其他文献
ARITA Jun的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('ARITA Jun', 18)}}的其他基金
Identification of the gene involved in the anti-proliferative action of estrogen
鉴定参与雌激素抗增殖作用的基因
- 批准号:
24590297 - 财政年份:2012
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular mechanism for the interaction between estrogen receptor and G protein-coupled receptor
雌激素受体与G蛋白偶联受体相互作用的分子机制
- 批准号:
21590254 - 财政年份:2009
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Rat strain difference in estrogen responsiveness of cell proliferation
大鼠品系对细胞增殖雌激素反应性的差异
- 批准号:
19590224 - 财政年份:2007
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Identification of estrogen-responsive cells involved in cell proliferation in response to estrogen
鉴定参与雌激素反应的细胞增殖的雌激素反应细胞
- 批准号:
15590206 - 财政年份:2003
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Conversion from stimulation to inhibition of the action of estrogen on cell proliferation
雌激素对细胞增殖的作用由刺激转为抑制
- 批准号:
13670058 - 财政年份:2001
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Intracellular signal transduction pathways regulating proliferation of anterior pituitary cells
调节垂体前叶细胞增殖的细胞内信号转导通路
- 批准号:
10670060 - 财政年份:1998
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Identification of the hypothalamic hormone that regulates proliferatin of lactorophs
调节乳酸菌增殖的下丘脑激素的鉴定
- 批准号:
08670081 - 财政年份:1996
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Changes in lactotroph responsiveness to secretagogues as determined by the sequential cell immunoblot assay
通过序贯细胞免疫印迹测定确定泌乳素对促分泌素反应性的变化
- 批准号:
03670081 - 财政年份:1991
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似海外基金
EFR3: Novel gatekeeper of cell proliferation
EFR3:细胞增殖的新型看门人
- 批准号:
DP240102729 - 财政年份:2024
- 资助金额:
$ 2.24万 - 项目类别:
Discovery Projects
Modulation of cell proliferation by the tumor suppressor protein NRK
肿瘤抑制蛋白 NRK 对细胞增殖的调节
- 批准号:
23K08817 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
MR imaging for the cell proliferation using in vivo click chemistry
使用体内点击化学进行细胞增殖的 MR 成像
- 批准号:
23K18279 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Project 1: Determine the mechanisms Cyclin D-Cdk4/6 uses to drive cell proliferation
项目 1:确定 Cyclin D-Cdk4/6 驱动细胞增殖的机制
- 批准号:
10867552 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Identification of active components of sillkworm serum exhibiting cell proliferation and elucidation of the mechanism
蚕血清中细胞增殖活性成分的鉴定及其机制的阐明
- 批准号:
23KJ1691 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for JSPS Fellows
The Role of Arginine Transport on Pancreatic Alpha Cell Proliferation and Function
精氨酸转运对胰腺α细胞增殖和功能的作用
- 批准号:
10678248 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Regulation of Adherent Cell Proliferation by Matrix Viscoelasticity
基质粘弹性对贴壁细胞增殖的调节
- 批准号:
10735701 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Mechanisms of cell proliferation in whole-genome doubled cells
全基因组加倍细胞的细胞增殖机制
- 批准号:
10467183 - 财政年份:2022
- 资助金额:
$ 2.24万 - 项目类别:
mechanism of cancer cell proliferation in bone microenvironment of renal cell carcinoma
肾细胞癌骨微环境中癌细胞增殖机制
- 批准号:
22K09370 - 财政年份:2022
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
mRNA selective translational control required for B cell proliferation
B 细胞增殖所需的 mRNA 选择性翻译控制
- 批准号:
2757728 - 财政年份:2022
- 资助金额:
$ 2.24万 - 项目类别:
Studentship